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Background/purpose: Taiwan's dentistry entered into a new era of modernization and flourished during the Japanese colonial period. However, we know very little about the composition of dentists at that time. This study attempted to analyze the ethnicity, gender, and geographical distribution of dentists in Taiwan in 1939 (Showa 14). Materials and methods: The methods of documentary analysis and secondary data analysis were adopted to find the composition of dentists during the late Japanese colonial period through a name list of contract dentists for the Postal Savings Insurance published in August 1939 (Showa 14) by the Taiwan Government Transportation Department Information Bureau. Results: The total number of contract dentists was 368, accounting for 86.79% of the 424 practicing dentists in Taiwan in 1939 (Showa 14). Of the 368 contract dentists (328 males and 40 females), 225 (61.14%) were Taiwanese and 143 (38.86%) were Japanese. Among the 8 prefectures in Taiwan, Tainan Prefecture had the largest number of dentists (97), followed by Taipei (84) and Taichung (78) prefectures. The number of contract dentists per 100,000 people was 6.24, equivalent to 16,021 people served by each contract dentist in 1939 (Showa 14). The chi-square test for the trend analysis of gender distribution indicated a significantly higher proportion of male than female contract dentists in either Taiwanese or Japanese ethnic group. Conclusion: In the late Japanese colonial period, the number of Taiwanese dentists exceeded that of Japanese dentists. Furthermore, there were more male than female dentists in either the Taiwanese or the Japanese population.

The concept of the oral-systemic link is important in both basic and clinical dentistry. The microbiome (microbiota) and exosomes are two prevalent issues in the modern medical researches. The common advent of oral and general microbiological investigation originated from the initial observations of oral bacteria within the dental plaque known as oral microbiome. In addition to oral diseases related to oral microbiome, the disruption of the oral and intestinal microbiome could result in the onset of systemic diseases. In the past decade, the exosomes have emerged in the field of the medical researches as they play a role in regulating the transport of intracellular vesicles. However, with the rapid advancement of exosomes researches in recent years, oral tissues (such as dental pulp stem cells and salivary gland cells) are used as the research materials to further promote the development of regenerative medicine. This article emphasized the importance of the concept of the oral-systemic link through the examples of microbiome (microbiota) and exosomes. Through the researches related to microbiome (microbiota) and exosomes, many evidences showed that as the basic dentistry developed directly from the assistance of the basic medicine, indirectly the progress of the basic dentistry turns back to promote the development of the basic medicine, indicating the importance of the concept of the oral-systemic link. The understanding of the oral-systemic link is essential for both clinicians and medical researchers, regardless of their dental backgrounds.

Background/purpose: Taiwan’s eight dental schools are all located in the western region. This study attempted to analyze the relationship between the geographical distribution of dentists in Taiwan and their graduation school background and to develop several statistical indicators to analyze the geographical distribution of dentists. Materials and methods: The method of the secondary data analysis was adopted to collect the open information related to the number of the practicing dentists based on their graduation schools and practice locations by cities and counties in Taiwan in January 2023. The data were used to develop several statistical indicators to analyze the geographical distribution of dentists. Results: There were 16,533 practicing dentists in Taiwan in January 2023. Of the 16,533 dentists, 15,687 (94.88 %) graduated from domestic dental schools and 846 (5.12 %) graduated from foreign dental schools. For the school location, the coefficient of variation, Gini coefficient, population proportion index, and population density index were the largest (168.57 %, 0.47, 10.76 %, and 3927.01 people/square kilometer) in the dentists graduating from the northern dental schools. In overall, the dentists were concentrated in municipalities, cities, and the northern region of Taiwan, especially those graduating from the northern dental schools. Conclusion: In Taiwan, the geographical distribution of dentists is highly related to their graduation school background. Promoting a balanced distribution of dental enrollment quotas and dental student sources from different geographical locations and inducing the movement of dentists to the dentist-shortage areas may be the more feasible ways to solve the uneven geographical distribution of dentists in Taiwan.

Background/purpose: Dental caries is one of the most common oral diseases in children worldwide, especially in Taiwan. This study investigated dental professionally topical fluoride application (PTFA) and children's dental use for dental caries under the National Health Insurance (NHI) system in Taiwan from 2008 to 2021. Materials and methods: The population data and medical records of the NHI system were obtained from the websites of the Ministry of the Interior and the Ministry of Health and Welfare, respectively. The dental PTFA services and dental use indicators for dental caries were analyzed from 2008 to 2021. Results: The number of out-patient visits for dental PTFA services increased from 221,675 in 2008 to 1,078,099 in 2021. The total increase of the out-patient visits was 856,424 with a total increase rate of 386.34%. The one-year increase was 65,879 with a one-year increase rate of 29.72%. Among the 3 age groups of children, their dental use indicators almost presented decreasing trends from 2008 to 2021. Moreover, in general, the dental use indicators showed a negative correlation with the number of the overall out-patient visits for dental PTFA services from 2008 to 2021. Conclusion: In Taiwan, there is a negative correlation between the dental use indicators in NHI system and the number of the overall out-patient visits for dental PTFA services from 2008 to 2021. However, the problem of dental caries in children is still serious and there is still a space for improvement in the oral health education for child caregivers and children.

Background/purpose: Oral cancer is a major cause of cancer morbidity and mortality worldwide, especially in Taiwan. This study investigated the morbidity and mortality of oral cancer in Taiwan from 2000 to 2021. Materials and methods: The population data and the records of cancer registry were obtained from the websites of the Ministry of the Interior and the Ministry of Health and Welfare, respectively. The numbers of oral cancer cases and deaths were analyzed from 2000 to 2021. Results: The numbers of oral cancer cases and deaths increased from 3378 to 1494 in 2000–8277 in 2020 and 3395 in 2021, respectively. The total increase of oral cancer cases was 4899 with a total increase rate of 145.03%, while the total increase of oral cancer deaths was 1901 with a total increase rate of 127.24%. The changes in the numbers of all cancer cases and deaths, as well as the morbidity and mortality of oral cancer or all cancers also showed similar trends. Furthermore, the death-to-case ratio of oral cancers decreased from 44.23% in 2000 to 40.84% in 2020. The total decrease was 3.39% with a total decrease rate of 7.66%. Conclusion: In Taiwan, people's awareness of oral mucosal health is still inadequate. Obviously, there is still much room for improvement in the oral mucosal health education for our people. With the expertise and responsibility for taking good care of oral health of our people, the dental personnel should take active roles in the prevention and screening for oral cancers.

Background/purpose: In Taiwan, it has been 16 years since the policy of children's dental professionally topical fluoride application (PTFA) services was implemented. This study investigated the differences in the number of children's dental PTFA outpatient visits among cities and counties or different regions of Taiwan in 2021. Materials and methods: The population data and medical records of the number of children's dental PTFA outpatient visits were obtained from the websites of Ministry of the Interior and Ministry of Health and Welfare, respectively. The secondary data were divided by cities and counties to analyze the urban-rural gap and regional differences in the number of children's dental PTFA outpatient visits in Taiwan in 2021. Results: In Taiwan, municipalities had the largest number of dental PTFA visits (721,338, 66.91% of the total dental PTFA visits), while remote and outlying island counties had the smallest number of dental PTFA visits (84,509, 7.84%). The northern region had the largest number of dental PTFA visits (466,728, 43.29%), while outlying islands had the smallest number of dental PTFA visits (25,055, 2.32%). Based on the children aged 3–5 years, municipalities had the lowest dental PTFA use rate (171.70%), while remote and outlying island counties had the highest dental PTFA use rate (482.96%). Conclusion: In Taiwan, there is a higher number of children's dental PTFA outpatient visits in municipalities than in non-municipalities or remote and outlying island counties as well as in northern region than in each of other four regions (central, southern, and eastern regions and outlying islands).

Background/purpose: : Taiwanese get use to drinking hand-shaken beverages. However, excessive sugar intake is strongly associated with the occurrence of dental caries. This study evaluated the correlation between the beverage serving activities and the dental use of National Health Insurance (NHI) for common oral diseases in 2016. Materials and methods: Data on the population, the beverage serving activities, and the dental records of NHI were obtained from the related websites of government. These data were divided by cities and counties to investigate the beverage serving activities and the dental use of NHI for common oral diseases in 2016. Results: In Taiwan, the value of beverage consumption per person had a moderate to high positive correlation with the dental use rate, the number of out-patient visits per 1,000 people, and the medical expenses per person for common oral diseases. For dental caries, diseases of pulp & periapical tissues, and gingivitis & periodontal diseases, their Pearson’s r values were 0.52–0.61, 0.18–0.40, and 0.58–0.65, respectively. These results indicate that the higher the beverage consumption per capita, the higher the dental use per capita. Conclusion: There is a long-term benefit for oral health and systemic health through reducing or avoiding unnecessary excessive sugar intake. Therefore, from a cost-effectiveness viewpoint, Taiwan government should develop a better oral health care policy including a health education strategy of appropriate sugar intake to prevent dental caries and other non-communicable diseases in Taiwanese people.

Background/purpose: In Taiwan, dental schools have gradually become a popular choice for high school graduates to enter in the universities. This study mainly analyzed the various admission ways to enroll students into the School of Dentistry of National Taiwan University (SODNTU) from 2000 to 2021. Materials and methods: The data on the numbers of enrollees and those enrollees who had completed registration in the SODNTU from 2000 to 2021 were available online to the public and included in this study. Results: The total number of enrollees in the SODNTU over the past 22 years was 764 with an annual mean of 34.73. The registration rate ranged from 90.91% to 100% with a mean registration rate of 96.86% from 2000 to 2021. The examination admission, individual application admission, and numerous stars initiatives admission were the three main admission ways to enroll students into the SODNTU from 2000 to 2021 with a total of 662 enrollees (86.65% of a total of 764 enrollees). The number (proportion) of enrollees through examination admission showed a decreasing trend year by year from 26 (78.79%) in 2000 to 15 (45.45%) in 2021. On the contrary, those enrollees through individual application admission and numerous stars initiatives admission showed an increasing trend year by year from 4 (12.12%) in 2000 to 14 (42.42%) in 2021. Conclusion: Although the examination admission is still the predominant admission way, the individual application admission and numerous stars initiatives admission gradually play pivotal roles to enroll students into the SODNTU recently.

Background/purpose: The development of dental assistants in Taiwan has been over a century. This study explored mainly the profile of dental assistants and the occupation survey about them in Taiwan from 2009 to 2021. Materials and methods: This study used the documentary analysis and secondary data analysis to survey mainly the changes of manpower of dental assistants in Taiwan from 2009 to 2021, and the association of their wages with economic indicators. Results: According to occupation survey from government, the number of dental assistants increased from 4315 in 2009–7909 in 2021. When estimated based on the data of the United States showing 1.86 dental assistants per dentist, the estimated number of dental assistants increased from 21,113 in 2009 to 28,698 in 2020. Although the mean monthly wage of dental assistants increased from 22,127 NT dollars in 2009 to 26,795 NT dollars in 2021, the proportion of the mean annual wage of dental assistants to the gross domestic product per capita decreased from 47.43% in 2009 to 34.78% in 2021. Moreover, the proportion of the mean monthly wage of dental assistants to the basic monthly wage of labor decreased from 128.05% in 2009 to 111.65% in 2021. Conclusion: In Taiwan, although the manpower and work content of dental assistants are important for dentists, they usually work with a low wage from 2009 to 2021. It is imperative to continue to promote the certification system for dental assistants to improve the professionalism and the wage structure of this occupation.

Background/purpose: Currently, Taiwan has a very complete education system of oral hygiene, but there is still no licensing system for oral hygienists. This study explored mainly the profile of education system for oral hygienists in Taiwan from 2017 to 2021. Materials and methods: This study used the documentary analysis and secondary data analysis to survey mainly the development of oral hygiene education system in Taiwan, and the changes of oral hygiene students and graduates from 2017 to 2021. Results: In Taiwan, currently, there are 8 oral hygiene schools which offer associate, bachelor, and master degrees in oral hygiene. The oral hygiene enrollment quotas determined by the Ministry of Education increased from 343 quotas in 9 programs in 2017 to 500 quotas in 13 programs in 2021, while new oral hygiene students who completed registration increased from 282 students in 2017 to 353 students in 2021. The registration rate of the five-year training program of junior college was significantly higher than the other programs. The number of oral hygiene students with official student status increased from 604 students in 2017 to 1349 students in 2021. The total increase was 745 students from 2017 to 2021 with a total increase rate of 123.34%. Conclusion: In Taiwan, although the manpower of oral hygienists is important for public oral health, they usually work as dental assistants or change their careers. It is imperative to continue to promote the certification system for oral hygienists to improve the professionalism of this occupation.

Background/purpose: During the Japanese colonial period, Taiwan had no dental school but had a medical school. This study explored the dental education and research activities in the medical school and special dentist qualification system in Taiwan during the Japanese colonial period. Materials and methods: This study analyzed the “related incidents and documents of dental education and research and dentist qualification system in Taiwan during the Japanese colonial period” and explored the dental education and research activities in the medical school and special dentist qualification system in Taiwan during the Japanese colonial period. Results: In 1914, Taiwan Government Medical School hired Dr. Kaname Ansawa, the earliest dental teacher in Taiwan, to teach dental courses in the medical school. In 1918, the “Theory of Dentistry” was considered to be the first independent “Dentistry” subject in the medical school. In 1936, the Faculty of Medicine of Taipei Imperial University listed “Dentistry & Oral Surgery” as an independent graduation examination subject. For dentist qualification system, a qualified physician who had finished dental courses and training could apply for a dental specialty license to work as a dentist. Taiwan Government Medical School (Dentistry) Research Department was the earliest department involved in the teaching and research of dentistry in the medical school and was also an educational institution for cultivating dental practitioners in Taiwan. Conclusion: In the Japanese colonial period, although no dental school was established in Taiwan, there were rich dental education and research activities in the medical school and a special dentist qualification system.

Background/purpose: During the Japanese colonial period, the Taipei Hospital had already provided complete dental services with a fixed price per treatment. This study tried to compare the differences in the prices of various dental treatments between the Japanese colonial period and today. Materials and methods: This study used the “Dental Treatment Fees in the 27th Annual Report of Taipei Hospital (Taisho 12)” as the study materials to compare the differences in the prices of various dental treatments between the Japanese colonial period and today using the monthly salary of a novice elementary school teacher as an income benchmark. Results: A hundred years ago, the Taipei Hospital had already provided the dental treatments such as scaling and endodontic, operative dentistry, prosthodontic, and orthodontic treatments. Of these treatment items, the prices for prosthodontic and orthodontic treatments were more expensive. After a century of development, the costs of scaling and operative dentistry treatments dropped, while the costs of endodontic, prosthodontic, and orthodontic treatments increased. Conclusion: During the Japanese colonial period, Taiwan's dental treatment technology had already had the forms of modern dentistry with several dental specialties. At that time, the costs of dental treatments are quite expensive. Today's dental treatment items are more detailed and diverse. The health insurance system provides Taiwanese people with convenient and cheap general dental treatments such as scaling and treatments related to operative dentistry, endodontics, periodontics, and oral surgery. However, the costs of prosthodontic and orthodontic treatments increase and have to be paid by the patients themselves.

Background/purpose: During the Japanese colonial period, Taiwan had a medical school education system for cultivating physicians, but did not have a dental school education system for cultivating “real” dentists. In this investigation, we collected and analyzed the historical documents related to dental education to study the development of dental education for medical students in Taiwan during the Japanese colonial period. Materials and methods: This study mainly analyzed the changes in the development of dental education for medical students in Taiwan during the Japanese colonial period through the collection and sorting of relevant historical materials. Results: During each stage of Taiwan's medical education system in the Japanese colonial period, the medical school offered compulsory dental courses for medical students, including theory and clinical practice of dentistry. Although there was no specific dental subject included in the graduation examination, evidence showed that the content of dentistry was covered by the subject of Surgery in the examination. Moreover, Taipei Imperial University established the Medical Faculty in 1936. Its curriculum increased the weight of dentistry and added the “Dentistry & Oral Surgery” as a graduation examination subject, indicating the importance of dental education for medical students in that period. Conclusion: In the Japanese colonial period, although there was no dental school for cultivating dentists in Taiwan, there was still dental education for medical students to let them understand the Dentistry and to enable them to become dental practitioners. This can be regarded as a workaround in the medical and healthcare policy.

Background/purpose: In 1906, there was a dental treatment room established in the department of surgery of Taipei Hospital to introduce modern dental services to Taiwan. It is undoubtedly the earliest hospital dentistry in Taiwan. This study tried to assess the dental manpower and treated dental diseases in Taipei Hospital, and hence to explore the appearance of Taiwan's early hospital dentistry. Materials and methods: This study utilized the secondary data analysis to evaluate the dental manpower and treated dental diseases of dental outpatients in the 27th Annual Report of Taipei Hospital (Taisho 12th year or 1923). Results: Our results found that there were 3 dentists and one dental technician in the dental department of Taipei Hospital in 1923. These 3 dentists totally treated 3 inpatients and 1759 outpatients with 4511 outpatient visits (1959 for male patients and 2552 for female patients) in that year. Dental caries and retained root were the two most common dental diseases in Japanese and Taiwanese dental outpatients, and followed in a descending order by missing tooth, pulp necrosis, and pulpitis for Japanese outpatients as well as pulpitis, suppurative periodontitis, and acute or chronic periodontitis for Taiwanese outpatients. Conclusion: We conclude that the dentistry of Taipei Hospital in 1923 does have a modern form of dentistry with 3 dentists and one dental technician. Its dental patients are mainly Japanese and minorly Taiwanese. Moreover, the treated dental diseases are mainly tooth diseases for Japanese outpatients and both tooth and periodontal diseases for Taiwanese outpatients.

Background/purpose: In 1896, the first dentist opened his dental clinic in Taipei City and this also opened a new era of Taiwan's dentistry. This study tried to assess the dentist manpower in Taiwan during the Japanese colonial period from 1923 to 1924, and hence to explore the appearance of Taiwan's early dentistry. Materials and methods: This study utilized the secondary data analysis to evaluate the numbers and distributions of dentists, physicians, and local practitioners in Taiwan from 1923 to 1924, to find the relationship among them, and further to explore the development of dentists in Taiwan at that period. Results: The total numbers of dentists and physicians increased from 87 to 882 in 1923 to 97 and 927 in 1924, respectively. Moreover, the total number of local practitioners decreased from 583 in 1923 to 558 in 1924. Their Gini coefficients for dentists, physicians, and local practitioners were 0.18, 0.16 and 0.20 in 1923 and 0.27, 0.05 and 0.19 in 1924, respectively. From 1923 to 1924, dentists and physicians were mainly concentrated in the northern and southern regions of Taiwan, and dentists had more serious uneven distribution problem. Conclusion: We conclude that the uneven distribution of dentist in Taiwan is an old problem. After a hundred years of development, the number of dentists increases at a higher rate than the number of physicians and there is no shortage of dentists to date in Taiwan. However, the problem of uneven distribution of dentists still exists and is even more serious now.

Background/purpose: Adjuvant chemotherapy has been used to control the primary oral squamous cell carcinoma (OSCC) size prior to surgical excision of the cancer. This study aimed to explore the histological changes of primary OSCCs and their cervical lymph node metastatic cancer lesions after chemotherapy. Materials and methods: Thirty-three OSCC patients with eleven having cervical lymph node metastases received adjuvant chemotherapy before surgical excision of their cancer lesions. Hematoxylin and eosin-stained tissue sections of incisional biopsy, surgical excision, and cervical lymph node metastatic cancer lesion specimens were compared microscopically to observe the histological changes in the cancer tissues after chemotherapy. Results: Common histological features could be found in the primary OSCCs and their cervical lymph node metastatic cancer lesions after chemotherapy. These included direct killing of cancer cells by chemotherapeutic agents, resulting in cancer cell necrosis and degeneration in the early phase, and squamous and keratinizing metaplasia of drug-induced cancer cells, leading to individual cell keratinization and keratin pearl formation in the later phase. There were also small nests of drug-resistant proliferating cancer cells in the inflamed fibrous connective tissue stroma. The most characteristic histological feature in the metastatic lymph nodes after chemotherapy was the keratinizing metaplasia of the metastatic cancer cells, resulting in the formation of epidermoid cyst-like lesions. Conclusion: Although the cancer reduces its size after chemotherapy, residual cancer cells are consistently present in the primary OSCC lesions after chemotherapy. Therefore, wide surgical resection of the cancer is still needed to ensure the complete removal of all cancer tissues.

Background/purpose: The dentists are the high-risk group of infectious respiratory diseases during dental treatment. This study investigated the changes of the numbers of overall practicing dentists, dental clinics, and hospital dentists in Taiwan during the COVID-19 pandemic. Materials and methods: We collected the data of the numbers of overall practicing dentists, dental clinics, and hospital dentists in each city and county of Taiwan in October 2019, April 2020, and October 2020 for evaluating the impact of the COVID-19 pandemic on the dentist manpower in Taiwan. Results: After the COVID-19 outbreak, the total increased numbers of practicing dentists, dental clinics, and hospital dentists in Taiwan were 408, 14, and −16 from October 2019 to October 2020, respectively. In addition, their increased rates of the corresponding items were 2.76%, 0.21%, and −0.72%, respectively. The increased rate of practicing dentists from October 2019 to April 2020 was significantly higher than that of dental clinics (P

Background/purpose: There were 661 board-certified orthodontic specialists in Taiwan in 2020. This study reported the current practice patterns and training project of orthodontic specialist in Taiwan. Materials and methods: This study exploited the secondary data analysis and interview to survey the current practice patterns and training project of orthodontic specialists in Taiwan. Results: Of the 661 orthodontic specialists, there were 84 registered in the medical centers, 44 in the regional hospitals, 7 in the district hospitals, and 526 in the dental clinics, respectively. There were 20 orthodontic specialist training institutions (15 medical centers, 3 regional hospitals, one district hospital, and one dental clinic) that had 218 dentists in orthodontic departments of training institutions and a training capacity of 51 dentists. Of the 20 orthodontic specialist training institutions in Taiwan in 2020, 12 were located in the northern, 4 in the central, and 4 in the southern regions of Taiwan. Moreover, we found none of the training institutions in the eastern and offshore island regions of Taiwan and the number of the orthodontic specialists did increase along with the population and the number of the practicing dentists. Conclusion: There are three major orthodontic specialist occupation problems in Taiwan: a surplus of the orthodontic specialists in the near future, an uneven distribution of the orthodontic specialists, and a concentration of the orthodontic specialists in the metropolitan areas of the municipalities. Therefore, we suggest that the government and relevant professional groups should propose solutions for these problems as soon as possible.

Background/purpose: Currently, very few young dentists in Taiwan are willing to choose oral pathology specialist as their lifelong career. This study reported the views of young dentists on the profession of oral pathology. Materials and methods: This study exploited the observational method and the questionnaire survey to collect the views of young dentists on the profession of oral pathology in Taiwan. Results: Thirty-five dental trainees or residents filled out the questionnaires. They agreed that the life quality or workload, interest, incidence of medical disputes, accomplishment, future job opportunities, salary level, and possibility to become a clinic owner were important factors that were considered to choose a dental specialist training program. Most (33, 94.3%) of them were sure that they would not choose oral pathology specialists as their lifelong career. The important reasons for not choosing oral pathology specialists as their lifelong career were learning characteristics, insufficient understanding of the work that an oral pathology specialist has to do, poor life quality or heavy workload, lower accomplishment, fewer job opportunities, and a relatively lower salary. The important factors that could increase the young dentists’ willingness to consider oral pathology specialist as the lifelong career included increased income, life quality, and future job opportunities and reduced workload for the oral pathology residents and specialists. Conclusion: We suggest that the government should build a long-term budget to subsidize oral pathology residents and specialists to further solve the serious oral pathology specialist shortage problem in Taiwan.

Background/purpose: Online learning has been used to continue dental education activities during the coronavirus disease 2019 (COVID-19). This study aimed to compare the learning effectiveness between physical classroom and online class learning for dental education during the COVID-19 pandemic by the questionnaire survey. Materials and methods: A questionnaire-based online survey was used to evaluate the learning effectiveness on the dental students who took the compulsory course entitled “oral pathology and diagnosis” in School of Dentistry, National Taiwan University in 2021. Student's t-test and Mann–Whitney U test were used to analyze the difference in learning effectiveness between physical classroom and online class learning for dental education during the COVID-19 pandemic. Results: In this study, dental students tended to have a viewpoint that the learning effectiveness of online class learning was better than that of physical classroom learning. On the contrary, they tended to have a viewpoint that the convenience and fairness of physical classroom examination was better than that of online examination (all P-values

Background/Purpose: Virtual microscopy has been used for teaching general and oral pathology laboratory course for more than 10 years. This study aimed to share the learning experience of an oral pathology laboratory course using either the virtual microscopy with digitalized virtual slides (virtual slide learning) or real microscopy using traditional glass slides (glass slide learning) among dental students. Materials and methods: Thirty-eight undergraduate dental students who took the compulsory course entitled “oral pathology” in the School of Dentistry, National Taiwan University were included in this study. The questionnaires were filled and final test was taken by these students after finishing the teaching of oral pathology laboratory course using either virtual or glass slide learning. The data were collected and analyzed statistically. Results: Our results showed a significantly higher acceptance rate (all P-values