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Your search keyword '"Lee, Chi-Yu"' showing total 11 results
Start Over Author "Lee, Chi-Yu" Publisher elsevier
11 results on '"Lee, Chi-Yu"'

9. Monoclonal antibodies to human sperm antigens -- II

Author

Department of Obstetrics and Gynaecology, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Obstetrics and Gynaecology, The University of British Columbia, Vancouver, Canada, Lee, Chi-Yu Gregory, Wong, Eric, Richter, Diane E., Menge, Alan C., Department of Obstetrics and Gynaecology, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Obstetrics and Gynaecology, The University of British Columbia, Vancouver, Canada, Lee, Chi-Yu Gregory, Wong, Eric, Richter, Diane E., and Menge, Alan C.

Abstract

As part of our continuous effort to elucidate the biochemical and immunological nature of human sperm surface antigens, monoclonal antibodies to human spermatozoa were generated by improved hybridoma techniques. Following immunizations with the membrane fraction of human spermatozoa and cell fusions, hybrid cells were cultured in a semi-solid HAT-selection medium to maximize the number of monoclones recovered. Subcultures were made in liquid phase 7 to 10 days after cell fusions by removing colonies from the initial medium. Based on the results of screening by microplate enzyme-linked immunoassay, 143 of 552 initial clones were found to secrete antibodies to human sperm antigens. More than one-hundred independently derived hybrid cell lines were established. Using indirect immunofluorescent procedures, 62 cell lines were shown to produce antibodies to surface antigens of human spermatozoa. Unique sperm antigens that react with monoclonal antibodies were identified by the SDS gel/protein blot radioimmunobinding method. Sperm agglutinating and immobilizing antibodies were exhibited by 4 and 15 hybrid cell lines, respectively. Fourteen of the monoclonal antibodies also exhibited cross-reactivity with methanol-fixed sperm cells of the rabbit or mouse or both whereas a reaction was not seen with viable sperm of these species. Generation of monoclonal antibodies against a wide spectrum of human sperm antigens should facilitate future investigations regarding immunologic-associated human infertility and fertility control.

Published
2006

10. Immunological relatedness of two isozymes of 3-phosphoglycerate kinase from the mouse

Author

Department of Human Genetics, University of Michigan, Ann Arbor, MI 48, 109, USA, Laboratories of Environmental Mutagenesis and Biochemical Genetics, National Institute of Environmental Health Sciences Research Triangle Park, NC 27709 USA, Pegoraro, Bruna, Ansari, Aftab A., Lee, Chi-Yu Gregory, Erickson, Robert P., Department of Human Genetics, University of Michigan, Ann Arbor, MI 48, 109, USA, Laboratories of Environmental Mutagenesis and Biochemical Genetics, National Institute of Environmental Health Sciences Research Triangle Park, NC 27709 USA, Pegoraro, Bruna, Ansari, Aftab A., Lee, Chi-Yu Gregory, and Erickson, Robert P.

Published
2006

11. Distribution and hosts of arsenic in a sediment core from the Chianan Plain in SW Taiwan: Implications on arsenic primary source and release mechanisms.

Author

Yang HJ, Lee CY, Chiang YJ, Jean JS, Shau YH, Takazawa E, and Jiang WT

Subjects
Environmental Monitoring, Groundwater chemistry, Rivers chemistry, Taiwan, Arsenic analysis, Carbon analysis, Geologic Sediments analysis, Water Pollutants, Chemical analysis
Abstract

High arsenic abundance of 50-700μg/L in the groundwater from the Chianan Plain in southwestern Taiwan is a well-known environmental hazard. The groundwater-associated sediments, however, have not been geochemically characterized, thus hindering a comprehensive understanding of arsenic cycling in this region. In this study, samples collected from a 250m sediment core at the centre of the Chianan Plain were analyzed for arsenic and TOC concentrations (N=158), constituent minerals (N=25), major element abundances (N=105), and sequential arsenic extraction (N=23). The arsenic data show a prevalence of >10mg/kg with higher concentrations of 20-50mg/kg concentrated at 60-80 and 195-210m. Arsenic was extracted mainly as an adsorbate on clay minerals, as a co-precipitate in amorphous iron oxyhydroxide, and as a structural component in clay minerals. Since the sediments consist mainly of quartz, chlorite, and illite, the correlations between arsenic concentration and abundances of K2O and MgO pinpoint illite and chlorite as the major arsenic hosts. The arsenic-total iron correlation reflects the role of chlorite along with the contribution from amorphous iron oxyhydroxide as indicated by arsenic extraction data. Organic matter is not the dominant arsenic host for low TOC content, low arsenic abundance extracted from it, and a relatively low R(2) of the arsenic-TOC correlation. The major constituent minerals in the sediments are the same as those of the upriver metapelites, establishing a sink-source relationship. Composition data from two deep groundwater samples near the sediment core show Eh values and As(V)/As(III) ratios of reducing environments and high arsenic, K, Mg, and Fe contents necessary for deriving arsenic from sediments by desorption from clay and dissolution of iron oxyhydroxide. Therefore, groundwater arsenic was mainly derived from groundwater-associated sediments with limited contributions from other sources, such as mud volcanoes., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Published
2016
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