Search

Very high levels of industrial contaminants in St. Lawrence Estuary (SLE) beluga whales represent one of the major threats to this population classified as endangered under the Species at Risk Act in Canada. Elevated concentrations of short-chained chlorinated paraffins (SCCPs) were recently reported in blubber of adult male SLE belugas. Recent regulations for SCCPs in North America, combined with their replacement by medium- (MCCPs) and long-chained chlorinated paraffins (LCCPs), highlight the importance of tracking this toxic chemical class. The objectives of this study were to evaluate (1) levels and profiles of chlorinated paraffins (CPs) in samples obtained from carcasses of adult male, adult female, juvenile, newborn, and fetus beluga, and (2) trends in adult male belugas between 1997 and 2018. Factors potentially influencing CP temporal trends such as age, feeding ecology and sampling year were also explored. SCCPs dominated (64 to 100%) total CP concentrations across all age and sex classes, MCCPs accounted for the remaining proportion of total CPs, and LCCPs were not detected in any sample. The chlorinated paraffin homolog that dominated the most in beluga blubber was C 12 Cl 8 . Adult male SCCP concentrations from this study were considerably lower (> 2000-fold) than those recently reported in Simond et al. (2020), likely reflecting a previously erroneous overestimate due to the lack of a suitable analytical method for SCCPs at the time. Both SCCPs and total CPs declined over time in adult males in our study (rate of 1.67 and 1.33% per year, respectively), presumably due in part to the implementation of regulations in 2012. However, there is a need to better understand the possible contribution of a changing diet to contaminant exposure, as stable isotopic ratios of carbon also changed over time., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Antoine Simond reports financial support was provided by Mitacs Canada. Dr. Tanya Brown reports financial support was provided by Fisheries and Oceans Canada., (Crown Copyright © 2023. Published by Elsevier B.V. All rights reserved.)

Elevated contaminant exposure has been identified as a stressor that has negative impacts on the health and recovery of the endangered St. Lawrence Estuary (SLE) beluga (Delphinapterus leucas) population. However, the accumulation of many groups of contaminants of emerging concern is still unknown in the SLE beluga. The objective of this study was to investigate the occurrence and temporal trends (2000-2017) of synthetic phenolic antioxidants (SPAs), secondary aromatic amines (Ar-SAs), benzotriazole UV stabilizers (BZT-UVs), and organic UV filters (UVFs) in the blubber (n = 69) and liver (n = 80) of SLE beluga carcasses recovered in the SLE. The SPA 2,6-di-tert-butyl-1,4-benzoquinone (BHTQ) was the most prevalent contaminant in the blubber (detection frequency: 86 %; median: 71.1 ng/g wet weight (ww)) and liver (50 %; 12.2 ng/g ww) of SLE belugas. In the blubber, 2-hydroxy-4-methoxybenzophenone (BP3) (36 %; 3.15 ng/g ww) and 2-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethyl butyl)phenol (UV329) (49 %; 6.84 ng/g ww) were the most frequently detected UVFs and BZT-UVs, respectively. Ar-SAs were not detected in most of the blubber and liver samples. Blubber accumulated higher levels of BHTQ and UV329 than liver, whereas the levels of BP3 were greater in the liver. Male SLE beluga accumulated greater concentrations of UV329 in blubber compared to females. These results indicated that the accumulation of BHTQ, UV329 and BP3 in SLE belugas is tissue- and sex-specific. BHTQ showed a decreasing trend in the blubber (2000-2017) of male SLE beluga, whereas no significant trend of this contaminant was found in females. UV329 showed no discernible temporal trend. This study established a baseline for the future monitoring of SPAs, Ar-SAs, BZT-UVs and UVFs in belugas and other marine mammals., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The endangered St. Lawrence Estuary (SLE) beluga population is declining and has shown no sign of recovery over the past decades despite several protective measures. Changes in the availability of food resources and exposure to organohalogen contaminants have been suggested as potential factors limiting the recovery of this population. Studies on SLE belugas have suggested that contaminant exposure may perturb energy metabolism, however, whether this translates into changes in energy reserves (lipid composition) and body condition is unknown. The objective of this study was to investigate the relationships between body condition and concentrations of organohalogens (polychlorinated biphenyls, organochlorine pesticides, and flame retardants) and a range of lipid metabolites (fatty acids, acylcarnitines, lysophosphatidylcholines, phosphatidylcholines, and sphingomyelins) in blubber samples collected from 51 SLE beluga carcasses recovered between 1998 and 2016 for which the cause of mortality was documented. Blubber Σ 9 fatty acid concentrations in SLE belugas significantly decreased between 1998 and 2016, suggesting a decline in energy reserves over the past two decades. Concentrations of several phosphatidylcholine analogues were greater in blubber of beluga males and/or females that were in poor body condition compared to those in good body condition. Moreover, concentrations of phosphatidylcholine acyl-alkyl C32:2 were greater in females that died from primary starvation (poor body condition). Greater concentrations of Σ 12 emerging flame retardants were also found in blubber of SLE beluga females that were in poorer body condition. This study suggests that the use of membrane lipids including phosphatidylcholine concentrations may be a good indicator of body condition and energy reserve status in blubber of marine mammals., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Exposure to oil can have long-term impacts on migratory birds. Following the 2010 Deepwater Horizon blowout in the Gulf of Mexico (GOM), we investigated potential impacts of oil exposure on a population of northern gannets (Morus bassanus) that breed on Bonaventure Island (Québec, Canada) and winter in GOM and along the U.S. Atlantic coast (AC). Blood and feather samples were collected from adults previously equipped with geolocators to determine wintering locations. Parent and alkylated polycyclic aromatic hydrocarbons (PAHs); trace metals; stable isotopes of carbon, nitrogen, and hydrogen; and immune, thyroid, steroid, retinoid, and genetic endpoints were measured. PAH and trace metal concentrations did not differ between gannets using different wintering sites. Feather stable isotope values varied significantly between birds from different wintering locations. Gannets wintering in GOM showed higher feather corticosterone and plasma thyroid hormone levels, which may indicate increased energetic demands and/or greater exposure to environmental stressors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

The development of integrated ecotoxicological approaches is of great interest in the investigation of global concerns such as impacts of municipal wastewater effluents on aquatic ecosystems. The objective of this study was to investigate the effects of a major wastewater municipal effluent on fish using a multi-level biological approach, from gene transcription and enzyme activities to histological changes. Yellow perch (Perca flavescens) were selected based on their wide distribution, their commercial and recreational importance, and the availability of a customized microarray. Yellow perch were sampled upstream of a major municipal wastewater treatment plant (WWTP) and 4 km and 10 km downstream from its point of discharge in the St. Lawrence River (Quebec, Canada). Concentrations of perfluoroalkyl substances (PFASs), polybrominated diphenyl ethers (PBDEs) and metals/trace elements in whole body homogenates were comparable to those from other industrialized regions of the world. Genomic results indicated that the transcription level of 177 genes was significantly different (p<0.024) between exposed and non-exposed fish. Among these genes, 38 were found to be differentially transcribed at both downstream sites. Impacted genes were associated with biological processes and molecular functions such as immunity, detoxification, lipid metabolism/energy homeostasis (e.g., peroxisome proliferation), and retinol metabolism suggesting impact of WWTP on these systems. Moreover, antioxidant enzyme activities were more elevated in perch collected at the 4 km site. Biomarkers of lipid metabolism, biosynthetic activity, and aerobic capacities were significantly lower (p<0.05) in fish residing near the outfall of the effluent. Histological examination of the liver indicated no differences between sites. Correlations between PFAS, PBDE, and metal/trace element tissue concentrations and markers of peroxisomal proliferation, oxidative stress, and retinoid metabolism were found at the gene and cellular levels. Present results suggest that relating transcriptomic analyses to phenotypic responses is important to better understand impacts of environmental contamination on wild fish populations., (Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.)

Climate change is influencing the structure and function of natural ecosystems around the world, including host-parasite interactions and disease emergence. Understanding the influence of climate change on infectious disease at temperate and tropical latitudes can be challenging because of numerous complicating biological, social, and political factors. Arctic and Subarctic regions may be particularly good models for unraveling the impacts of climate change on parasite ecology because they are relatively simple systems with low biological diversity and few other complicating anthropogenic factors. We examine some changing dynamics of host-parasite interactions at high latitudes and use these to illustrate a framework for approaching understanding, preventing, and mitigating climate change impacts on infectious disease, including zoonoses, in wildlife.