1. Effect of peptide-carrier coupling on peptide-specific immune responses.
- Author
-
Kirkley JE, Goldstein AL, and Naylor PH
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Cross Reactions, Cross-Linking Reagents, Female, Glutaral, HIV Antibodies biosynthesis, HIV Antigens administration & dosage, HIV Antigens chemistry, HIV Antigens immunology, Hemocyanins administration & dosage, Hemocyanins chemistry, Hemocyanins immunology, Humans, Immunoconjugates chemistry, Lymphocyte Activation, Maleimides, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptides chemistry, gag Gene Products, Human Immunodeficiency Virus, Immunoconjugates administration & dosage, Immunoconjugates immunology, Peptides administration & dosage, Peptides immunology
- Abstract
Synthetic peptides are covalently linked to immunogenic carrier proteins to enhance the anti-peptide immune response. To investigate whether the method of conjugation influences the immune response, we evaluated two distinctly different choices of linker for a peptide-carrier construct. HPG-30, a synthetic peptide derived from the p17 gag protein of human immunodeficiency virus 1, was covalently linked to keyhole limpet hemocyanin by either glutaraldehyde or a maleimide ester. Glutaraldehyde linkage enhanced the anti-peptide antibody and native protein response compared to maleimide. The maleimide-linked conjugate was more effective at inducing a peptide-specific cellular response. Thus, manipulation of the conjugation method can modify the magnitude and character of the immune response to a synthetic peptide vaccine.
- Published
- 2001
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