15 results on '"Kim, Woo Taek"'
Search Results
2. Arabidopsis Tóxicos en Levadura 78 (AtATL78) mediates ABA-dependent ROS signaling in response to drought stress.
- Author
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Suh JY, Kim SJ, Oh TR, Cho SK, Yang SW, and Kim WT
- Subjects
- Adaptation, Physiological physiology, Abscisic Acid metabolism, Arabidopsis physiology, Droughts, Signal Transduction physiology, Stress, Physiological physiology, Ubiquitin-Protein Ligases metabolism
- Abstract
Plants have developed a variety of complicated responses to cope with drought, one of the most challenging environmental stresses. As a quick response, plants rapidly inhibit stomatal opening under the control of abscisic acid (ABA) signaling pathway, in order to preserve water. Here, we report that Arabidopsis Tóxicos en Levadura (ATL), a RING-type E3 ubiquitin ligase, mediates the ABA-dependent stomatal closure. In contrast to wild-type plants, the stomatal closure was fully impaired in atatl78 mutant plants even in the presence of exogenous ABA and reactive oxygen species (ROS). Besides, under high concentrations of Ca(2+), a down-stream signaling molecule of ABA signaling pathway, atatl78 mutant plants successfully closed the pores. Furthermore, AtATL78 protein indirectly associated with catalases and the deficiency of AtATL78 led the reduction of catalase activity and H2O2, implying the function of AtATL78 in the modulation of ROS activity. Based on these results, we suggest that AtATL78 possibly plays a role in promoting ROS-mediated ABA signaling pathway during drought stress., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
3. Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.).
- Author
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Byun MY, Cui LH, and Kim WT
- Subjects
- Genomic Instability physiology, Plants, Genetically Modified physiology, Arabidopsis Proteins genetics, DNA Helicases genetics, Gene Expression Regulation, Developmental physiology, Gene Expression Regulation, Plant physiology, Oryza genetics, Oryza growth & development, Telomere Homeostasis genetics
- Abstract
The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
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4. PUB22 and PUB23 U-BOX E3 ligases directly ubiquitinate RPN6, a 26S proteasome lid subunit, for subsequent degradation in Arabidopsis thaliana.
- Author
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Cho SK, Bae H, Ryu MY, Wook Yang S, and Kim WT
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Droughts, Enzyme Stability, Gene Knockout Techniques, Genes, Plant, Plants, Genetically Modified, Proteasome Endopeptidase Complex chemistry, Proteasome Endopeptidase Complex genetics, Protein Subunits, Proteolysis, Recombinant Proteins genetics, Recombinant Proteins metabolism, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases deficiency, Ubiquitin-Protein Ligases genetics, Ubiquitination, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Proteasome Endopeptidase Complex metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
Drought stress strongly affects plant growth and development, directly connected with crop yields, accordingly. However, related to the function of U-BOX E3 ligases, the underlying molecular mechanisms of desiccation stress response in plants are still largely unknown. Here we report that PUB22 and PUB23, two U-box E3 ligase homologs, tether ubiquitins to 19S proteasome regulatory particle (RP) subunit RPN6, leading to its degradation. RPN6 was identified as an interacting substrate of PUB22 by yeast two-hybrid screening, and in vitro pull-down assay confirmed that RPN6 interacts not only with PUB22, but also with PUB23. Both PUB22 and PUB23 were able to conjugate ubiquitins on RPN6 in vitro. Furthermore, RPN6 showed a shorter protein half-life in PUB22 overexpressing plants than in wild-type, besides RPN6 was significantly stabilized in pub22pub23 double knockout plants. Taken together, these results solidify a notion that PUB22 and PUB23 can alter the activity of 26S proteasome in response to drought stress., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
5. Arabidopsis RING E3 ubiquitin ligase AtATL80 is negatively involved in phosphate mobilization and cold stress response in sufficient phosphate growth conditions.
- Author
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Suh JY and Kim WT
- Subjects
- Adaptation, Physiological, Arabidopsis metabolism, Arabidopsis physiology, Biomass, Cell Membrane enzymology, Phosphates metabolism, Phosphorus metabolism, Plants, Genetically Modified enzymology, Plants, Genetically Modified growth & development, Plants, Genetically Modified metabolism, Arabidopsis enzymology, Cold Temperature, Stress, Physiological, Ubiquitin-Protein Ligases metabolism
- Abstract
Phosphate (Pi) remobilization in plants is critical to continuous growth and development. AtATL80 is a plasma membrane (PM)-localized RING E3 ubiquitin (Ub) ligase that belongs to the Arabidopsis Tóxicos en Levadura (ATL) family. AtATL80 was upregulated by long-term low Pi (0-0.02 mM KH2PO4) conditions in Arabidopsis seedlings. AtATL80-overexpressing transgenic Arabidopsis plants (35S:AtATL80-sGFP) displayed increased phosphorus (P) accumulation in the shoots and lower biomass, as well as reduced P-utilization efficiency (PUE) under high Pi (1 mM KH2PO4) conditions compared to wild-type plants. The loss-of-function atatl80 mutant line exhibited opposite phenotypic traits. The atatl80 mutant line bolted earlier than wild-type plants, whereas AtATL80-overexpressors bloomed significantly later and produced lower seed yields than wild-type plants under high Pi conditions. Thus, AtATL80 is negatively correlated not only with P content and PUE, but also with biomass and seed yield in Arabidopsis. In addition, AtATL80-overexpressors were significantly more sensitive to cold stress than wild-type plants, while the atatl80 mutant line exhibited an increased tolerance to cold stress. Taken together, our results suggest that AtATL80, a PM-localized ATL-type RING E3 Ub ligase, participates in the Pi mobilization and cold stress response as a negative factor in Arabidopsis., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
6. Classification and interaction modes of 40 rice E2 ubiquitin-conjugating enzymes with 17 rice ARM-U-box E3 ubiquitin ligases.
- Author
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Bae H and Kim WT
- Subjects
- Oryza genetics, Phylogeny, Plant Proteins genetics, Ubiquitin-Conjugating Enzymes genetics, Ubiquitin-Protein Ligases genetics, Ubiquitination, Oryza metabolism, Plant Proteins metabolism, Protein Interaction Maps, Ubiquitin-Conjugating Enzymes metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
Rice, a monocot model crop, contains at least 48 putative E2 ubiquitin (Ub)-conjugating enzymes. Based on homology comparisons with 40 Arabidopsis E2 proteins and 35 human E2s, 48 rice E2s were classified into 15 different groups. Yeast two-hybrid analyses using the U-box-domain regions of armadillo (ARM)-U-box E3 Ub-ligases and the Ub-conjugating (UBC) domains of E2s showed that, among 40 rice E2s, 11 E2s accounted for 70% of the interactions with 17 ARM-U-box E3s. Thus, a single E2 could interact with multiple ARM-U-box E3s, suggesting the presence of E2 hubs for E2-E3 interactions in rice. Rice SPL11 ARM-U-box E3 displayed distinct self-ubiquitination patterns, including poly-ubiquitination, mono-ubiquitination, or no ubiquitination, depending on different E2 partners. This suggests that the mode of ubiquitination of SPL11 E3 is critically influenced by individual E2s., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
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7. Solution structure of CEH-37 homeodomain of the nematode Caenorhabditis elegans.
- Author
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Moon S, Lee YW, Kim WT, and Lee W
- Subjects
- Amino Acid Sequence, Animals, Binding Sites, Computer Simulation, Molecular Sequence Data, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Caenorhabditis elegans chemistry, Caenorhabditis elegans Proteins chemistry, Caenorhabditis elegans Proteins ultrastructure, Homeodomain Proteins chemistry, Homeodomain Proteins ultrastructure, Models, Chemical, Models, Molecular
- Abstract
The nematode Caenorhabditis elegans protein CEH-37 belongs to the paired OTD/OTX family of homeobox-containing homeodomain proteins. CEH-37 shares sequence similarity with homeodomain proteins, although it specifically binds to double-stranded C. elegans telomeric DNA, which is unusual to homeodomain proteins. Here, we report the solution structure of CEH-37 homeodomain and molecular interaction with double-stranded C. elegans telomeric DNA using nuclear magnetic resonance (NMR) spectroscopy. NMR structure shows that CEH-37 homeodomain is composed of a flexible N-terminal region and three α-helices with a helix-turn-helix (HTH) DNA binding motif. Data from size-exclusion chromatography and fluorescence spectroscopy reveal that CEH-37 homeodomain interacts strongly with double-stranded C. elegans telomeric DNA. NMR titration experiments identified residues responsible for specific binding to nematode double-stranded telomeric DNA. These results suggest that C. elegans homeodomain protein, CEH-37 could play an important role in telomere function via DNA binding., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
8. The N-terminal tetra-peptide (IPDE) short extension of the U-box motif in rice SPL11 E3 is essential for the interaction with E2 and ubiquitin-ligase activity.
- Author
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Bae H and Kim WT
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Binding Sites, Molecular Sequence Data, Mutagenesis, Site-Directed, Plant Proteins chemistry, Plant Proteins genetics, Proline metabolism, Two-Hybrid System Techniques, Ubiquitin-Conjugating Enzymes genetics, Ubiquitin-Protein Ligases genetics, Oryza metabolism, Plant Proteins metabolism, Ubiquitin-Conjugating Enzymes chemistry, Ubiquitin-Conjugating Enzymes metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
Rice, a monocot model plant, contains at least 77 U-box E3 ubiquitin (Ub)-ligases and 48 E2 Ub-conjugating enzymes. Here, we investigated the minimal binding domain of rice SPL11 U-box E3 to its E2 partners. Serial deletions and site-directed mutagenesis analyses indicated that, in addition to an intact U-box motif, the N-terminal tetra-peptide (IPDE) short extension of the U-box was essential for the interaction of SPL11 with E2s and Ub-ligase activity. The Ile and Pro residues at the -4 and -3 positions of the U-box, respectively, were crucial for this interaction. These results suggest that the N-terminal tetra-peptide extension of the U-box participates in the specific interaction of SPL11 E3 with E2s in a sequence-specific manner in rice., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
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9. Suppression of Arabidopsis RING-DUF1117 E3 ubiquitin ligases, AtRDUF1 and AtRDUF2, reduces tolerance to ABA-mediated drought stress.
- Author
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Kim SJ, Ryu MY, and Kim WT
- Subjects
- Amino Acid Motifs, Arabidopsis enzymology, Arabidopsis genetics, Arabidopsis Proteins genetics, Molecular Sequence Data, Mutation, Ubiquitin-Protein Ligases genetics, Arabidopsis physiology, Arabidopsis Proteins physiology, Droughts, RING Finger Domains, Stress, Physiological genetics, Ubiquitin-Protein Ligases physiology
- Abstract
Among approximately 480 RING domain-containing E3 Ub ligases in Arabidopsis, three, At3g46620, At5g59550, and At2g39720, have a domain-of-unknown-function (DUF) 1117 motif in their C-terminal regions. At3g46620 and At5g59550 were identified as homologous ABA- and drought-induced RING-DUF1117 genes and were designated AtRDUF1 and AtRDUF2, respectively. Single and double knock-out mutations of AtRDUFs resulted in hyposensitive phenotypes toward ABA in terms of germination rate and stomatal closure and markedly reduced tolerance to drought stress relative to wild-type plants. These results are discussed in the context that AtRDUF1 and AtRDUF2 play combinatorial, but still distinguishable, roles in ABA-mediated dehydration stress responses., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
10. Different prognostic significance of high on-treatment platelet reactivity as assessed by the VerifyNow P2Y12 assay after coronary stenting in patients with and without acute myocardial infarction.
- Author
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Ahn SG, Lee SH, Yoon JH, Kim WT, Lee JW, Youn YJ, Ahn MS, Kim JY, Yoo BS, Yoon J, and Choe KH
- Subjects
- Aged, Angioplasty, Balloon adverse effects, Angioplasty, Balloon mortality, Blood Platelets metabolism, Cardiovascular Diseases blood, Cardiovascular Diseases diagnostic imaging, Cardiovascular Diseases etiology, Cardiovascular Diseases mortality, Chi-Square Distribution, Coronary Angiography, Coronary Artery Disease blood, Coronary Artery Disease complications, Coronary Artery Disease diagnostic imaging, Coronary Artery Disease mortality, Female, Humans, Kaplan-Meier Estimate, Male, Middle Aged, Multivariate Analysis, Myocardial Infarction blood, Myocardial Infarction diagnostic imaging, Myocardial Infarction etiology, Myocardial Infarction mortality, Predictive Value of Tests, Proportional Hazards Models, Prospective Studies, Receptors, Purinergic P2Y12 blood, Recurrence, Republic of Korea, Risk Assessment, Risk Factors, Thrombosis blood, Thrombosis prevention & control, Time Factors, Treatment Outcome, Angioplasty, Balloon instrumentation, Blood Platelets drug effects, Cardiovascular Diseases prevention & control, Coronary Artery Disease therapy, Myocardial Infarction therapy, Platelet Aggregation Inhibitors therapeutic use, Platelet Function Tests, Purinergic P2Y Receptor Antagonists therapeutic use, Receptors, Purinergic P2Y12 drug effects, Stents
- Abstract
Objectives: This study compared the prognostic role of high on-treatment platelet reactivity (HTPR) in predicting thrombotic events in a Korean population undergoing percutaneous coronary intervention (PCI) in the acute myocardial infarction (AMI) and non-AMI setting., Background: The prognostic significance and optimal cutoff of HTPR might differ according to a given clinical condition, such as AMI and ethnicity., Methods: On-treatment platelet reactivity was measured with a VerifyNow P2Y12 assay (Accumetrics, San Diego, California) in 1,226 patients (824 men; age 65 ± 10 years), including 413 AMI cases, 12 to 24 h after PCI between March 2008 and March 2010. The prevalence of cardiovascular (CV) events defined as a composite of death from CV causes, nonfatal myocardial infarction, or stent thrombosis at 1-year follow-up were compared according to HTPR between patients with and without AMI., Results: The optimal cutoff for HTPR was 272 IU of the P2Y(12) reaction unit (PRU) (area under the curve: 0.708; 95% confidence interval [CI]: 0.607 to 0.809, p = 0.03), which was the upper-tertile threshold. Among AMI patients, 1-year CV events occurred more frequently in patients with versus without HTPR (n = 14 [8.8%] vs. n = 1 [0.4%], p < 0.001), whereas there was no difference in the composite endpoint on the basis of HTPR in patients without AMI (n = 7 [2.8%] vs. n = 8 [1.4%], p = 0.193)., Conclusions: Increased residual platelet reactivity is related to post-discharge CV events in subjects with AMI, whereas the prognostic significance of HTPR seems to be attenuated in patients with stable coronary disease after PCI., (Copyright © 2012 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
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11. Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits.
- Author
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Seo YS, Kim SJ, Harn CH, and Kim WT
- Subjects
- Alkyl and Aryl Transferases metabolism, Arabidopsis Proteins, Fruit enzymology, Fruit genetics, Fruit metabolism, Molecular Structure, Plant Leaves enzymology, Plant Leaves genetics, Plant Leaves metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, alpha-Tocopherol metabolism, Alkyl and Aryl Transferases genetics, Solanum lycopersicum genetics, Solanum lycopersicum metabolism, Malus genetics, Malus metabolism, alpha-Tocopherol analysis
- Abstract
Homogentisate phytyltransferase (HPT) is an important enzyme in the biosynthesis of tocopherols (vitamin E). Herein, an HPT homolog (MdHPT1) was isolated from apple (Malus domestica Borkh. cv. Fuji) fruits, whose gene expression level gradually decreased during fruit ripening, reaching a background level in ripened apple fruits. The amounts of α- and γ-tocopherols, two major tocopherols in plant organs, were 5- to 14-fold lower in the fruits than in the leaves and flowers of apple plants. Transgenic tomato plants (Solanum lycopersicum cv. Micro-Tom) overexpressing MdHPT1 were next constructed. Transgenic independent T(1) leaves contained ∼1.8- to 3.6-fold and ∼1.6- to 2.9-fold higher levels of α-tocopherol and γ-tocopherol, respectively, than those in control plants. In addition, the levels of α-tocopherol and γ-tocopherol in 35S:MdHPT1 T(1) fruits increased up to 1.7-fold and 3.1-fold, respectively, as compared to the control fruits, indicating that an increase in α-tocopherol in fruits (maximal 1.7-fold) was less evident than that in leaves (maximal 3.6-fold). This finding suggests that the apple MdHPT1 plays a role in tocopherol production in transgenic tomatoes., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
12. In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1.
- Author
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Son O, Cho SK, Kim SJ, and Kim WT
- Subjects
- Arabidopsis Proteins genetics, Cysteine Proteinase Inhibitors pharmacology, Leupeptins pharmacology, Plant Proteins genetics, Receptors, Cell Surface genetics, Nicotiana cytology, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Plant Proteins metabolism, Receptors, Cell Surface metabolism, Ubiquitin-Protein Ligases metabolism, Ubiquitination
- Abstract
E3 ubiquitin (Ub) ligases play diverse roles in cellular regulation in eukaryotes. Three homologous AtRmas (AtRma1, AtRma2, and AtRma3) were recently identified as ER-localized Arabidopsis homologs of human RING membrane-anchor E3 Ub ligase. Here, auxin binding protein 1 (ABP1), one of the auxin receptors in Arabidopsis, was identified as a potential substrate of AtRma2 through a yeast two-hybrid assay. An in vitro pull-down assay confirmed the interaction of full-length AtRma2 with ABP1. AtRma2 was transiently expressed in tobacco (Nicotiana benthamiana) plants through an Agrobacterium-mediated infiltration method and bound ABP1 in vivo. In vitro ubiquitination assays revealed that bacterially-expressed AtRma2 ubiquitinated ABP1. ABP1 was poly-ubiquitinated in tobacco cells and its stability was significantly increased in the presence of MG132, a 26S proteasome inhibitor. This suggests that ABP1 is controlled by the Ub/26S proteasome system. Therefore, AtRma2 is likely involved in the cellular regulation of ABP1 expression levels., (2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
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13. Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo.
- Author
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Kim ES, Yang SW, Hong DK, Kim WT, Kim HG, and Lee SK
- Subjects
- Carrier Proteins genetics, DNA genetics, DNA-Binding Proteins genetics, HeLa Cells, Humans, Polycomb Repressive Complex 1, Recombinant Fusion Proteins genetics, Saccharomyces cerevisiae Proteins genetics, Transcription Factors genetics, Carrier Proteins metabolism, DNA metabolism, DNA-Binding Proteins metabolism, Recombinant Fusion Proteins metabolism, Saccharomyces cerevisiae Proteins metabolism, Transcription Factors metabolism, Transduction, Genetic methods
- Abstract
Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 microg of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines., (Copyright (c) 2009 Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
14. Identification and characterization of three telomere repeat-binding factors in rice.
- Author
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Byun MY, Hong JP, and Kim WT
- Subjects
- Amino Acid Sequence, Dimerization, Electrophoretic Mobility Shift Assay, Molecular Sequence Data, Oryza genetics, Plant Proteins chemistry, Plant Proteins genetics, Protein Structure, Tertiary, Telomere-Binding Proteins chemistry, Telomere-Binding Proteins genetics, Two-Hybrid System Techniques, Oryza metabolism, Plant Proteins metabolism, Telomere metabolism, Telomere-Binding Proteins metabolism
- Abstract
Telomeres consist of nucleoprotein complexes that protect chromosome end structures. Here, we describe three OsTRBF genes, encoding telomere repeat-binding factors of the single Myb histone family in rice. The predicted proteins contain a Myb DNA-binding motif and a linker histone H1/H5 domain in the N-terminal and central regions, respectively. The OsTRBF transcripts were constitutively detected in rice plants grown under greenhouse conditions. Gel retardation assays showed that these OsTRBF proteins bind specifically to the plant double-stranded telomeric sequence, TTTAGGG, with markedly different binding affinities as judged by their respective dissociation constants. Yeast two-hybrid and in vitro pull-down assays indicated that both OsTRBF1 and OsTRBF2 interact with one another to form homo- and hetero-complexes, while OsTRBF3 appeared to act as a monomer. Our results suggest that OsTRBFs play combinatory roles in the function and structure of telomeres in rice.
- Published
- 2008
- Full Text
- View/download PDF
15. Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings.
- Author
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Lee JH, Deng XW, and Kim WT
- Subjects
- Amino Acid Oxidoreductases biosynthesis, Amino Acid Oxidoreductases genetics, Arabidopsis genetics, Arabidopsis growth & development, DNA-Binding Proteins, Darkness, Ethylenes pharmacology, Mutation, Seedlings genetics, Seedlings metabolism, Signal Transduction genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Gene Expression Regulation, Plant, Light, Nuclear Proteins metabolism, Transcription Factors metabolism
- Abstract
To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.
- Published
- 2006
- Full Text
- View/download PDF
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