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Your search keyword '"Jurat‐Fuentes, J. L."' showing total 3 results
Start Over Author "Jurat‐Fuentes, J. L." Publisher elsevier
3 results on '"Jurat‐Fuentes, J. L."'

1. List of Contributors

Author

Almeida, J., primary, Arthurs, S.P., additional, Baggio, M.V., additional, Bateman, R., additional, Becker, N., additional, Bruck, D.J., additional, Chandler, D., additional, Clifton, E.H., additional, Conlong, D., additional, Dara, S.K., additional, Dolinski, C., additional, Duncan, L.W., additional, Ekesi, S., additional, Faria, M., additional, Fusco, R., additional, Gassmann, A.J., additional, Glare, T.R., additional, Glazer, I., additional, Goble, T., additional, Gore, J., additional, Gray, E.W., additional, Grzywacz, D., additional, Gwynn, R., additional, Hajek, A.E., additional, Han, R., additional, Hazir, S., additional, Jackson, T.A., additional, Jaronski, S.T., additional, Jenkins, N., additional, Jurat-Fuentes, J.-L., additional, Koehler, P.G., additional, Kooyman, C., additional, Koppenhöfer, A.M., additional, Lacey, L.A., additional, Leite, L.G., additional, Leland, J., additional, Lopes, R.B., additional, Lüthy, P., additional, Maniania, N.K., additional, Mascarin, G.M., additional, Moore, D., additional, Moore, S., additional, Moore, S.D., additional, Nakai, M., additional, O’Callaghan, M., additional, Oi, D.H., additional, Pereira, R.M., additional, Peters, A., additional, Prior, C., additional, Shapiro-Ilan, D., additional, Solter, L.F., additional, Sosa-Gómez, D.R., additional, Su, T., additional, van Frankenhuyzen, K., additional, Wraight, S.P., additional, Wu, S., additional, and Yan, X., additional

Published
2017
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3. Monitoring stem cell proliferation and differentiation in primary midgut cell cultures from Heliothis virescens larvae using flow cytometry.

Author

Castagnola A, Eda S, and Jurat-Fuentes JL

Subjects
Animals, Cattle, Cell Separation methods, Cell Shape, Cells, Cultured, Gastrointestinal Tract anatomy & histology, Gastrointestinal Tract physiology, Stem Cells cytology, Cell Differentiation physiology, Cell Proliferation, Flow Cytometry methods, Larva anatomy & histology, Larva physiology, Moths anatomy & histology, Moths physiology, Stem Cells physiology
Abstract

In the midgut of Heliothis virescens larvae, proliferation and differentiation of stem cell populations allow for midgut growth and regeneration. Basic epithelial regenerative function can be assessed in vitro by purifying these two cell type populations, yet efficient high throughput methods to monitor midgut stem cell proliferation and differentiation are not available. We describe a flow cytometry method to differentiate stem from mature midgut cells and use it to monitor proliferation, differentiation and death in primary midgut stem cell cultures from H. virescens larvae. Our method is based on differential light scattering and vital stain fluorescence properties to distinguish between stem and mature midgut cells. Using this method, we monitored proliferation and differentiation of H. virescens midgut cells cultured in the presence of fetal bovine serum (FBS) or AlbuMAX II. Supplementation with FBS resulted in increased stem cell differentiation after 5 days of culture, while AlbuMAX II-supplemented medium promoted stem cell proliferation. These data demonstrate utility of our flow cytometry method for studying stem cell-based epithelial regeneration, and indicate that AlbuMAX II-supplemented medium may be used to maintain pluripotency in primary midgut stem cell cultures., (Copyright © 2010 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)

Published
2011
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