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It is well known that fabric of sand may significantly affect mechanical behaviors and liquefaction resistance of sand. Various optical techniques are currently utilized to visualize the fabric, especially the distribution of the long axis of soil particles. However, none of these methods provides an ideal solution in laboratory tests and in situ observation. In this study, anisotropy of magnetic susceptibility (AMS) was first proposed as a convenient and efficient way to evaluate the liquefaction of clean sand. At first, investigations with scanning electron microscopy (SEM) and AMS were simultaneously conducted on two groups of soil specimens with different initial fabrics to verify the feasibility of the AMS technique. Then, 80 in situ samples were collected to analyze the feature of liquefied and non-liquefied sand layers through AMS tests. It is clearly known from the test results that the natural sedimentary fabric was destroyed during liquefaction and the fabric anisotropy was greatly changed after liquefaction. The feasibility of evaluating soil fabric using the AMS survey was verified by the laboratory tests. Furthermore, the applicability of AMS in detecting liquefied layer in situ was confirmed for the first time.

Element tests, such as water retention tests and triaxial tests, are efficient and widely used methods for investigating the elementary hydro-mechanical behavior of unsaturated soil. In recent research, discrepancies observed in unsaturated test results using axis-translation techniques (ATTs) with ceramic disks and microporous membrane filters (MM filters) have indicated that misunderstandings might be hiding behind the perceived elementary behavior seen in element tests. In this study, triaxial tests with ceramic disks and MM filters were firstly conducted on specimens of unsaturated completely decomposed granite sand, referred to as Masado in Japan, to compare the influence of different techniques on the test results. Then a soil–water-air coupled finite element-finite difference (FE-FD) method, based on a newly proposed unsaturated/saturated constitutive model coupled with a deformation-dependent water retention curve (WRC), was utilized to simulate triaxial tests as boundary value problems (BVPs) because of the non-uniform deformation of the specimens. Drained/vented triaxial tests showed that the stress–strain relation of Masado is basically the same regardless of the applied technique, while the amount of drainage discharge and degree of saturation during the shearing stage are quite different. The calculated average stress–strain relation basically reproduced a tendency similar to that of the tests. It was also shown from the calculation that the ATT with ceramic disks or MM filters significantly influences the distributions of saturation and stress within the specimens during shearing. It should be emphasized that unsaturated triaxial tests with the ATT are basically BVPs instead of so-called element tests.

Our study was conducted to investigate the effects of the fruits of Lycium chinense Mill. (Lycii Fructus, LF) and its bioactive compound, betaine, on muscle differentiation and mitochondrial biogenesis in C2C12 cells. LF extract and betaine was analyzed by high-performance liquid chromatography (HPLC). The expression of myosin heavy chain (MyHC) and peroxisome proliferator-activated receptor gamma coactivator1-alpha (PGC-1α), sirtuin-1(Sirt-1), nuclear respiratory factor-1 (NRF-1), transcription factor A, mitochondrial (TFAM) and the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), were determined in cellular or mitochondrial levels by quantitative polymerase chain reaction (qPCR) or Western blot, respectively. The glucose levels and total ATP contents were measured by the glucose consumption in a culture medium, cellular glucose uptake and ATP assays. LF extract at 4 mg/ml and betaine at 2 and 5 mM significantly increased the expression of MyHC in C2C12 myotubes, compared with non-treated cells. LF extract and betaine significantly increased the expression of PGC-1α, Sirt-1, NRF-1 and TFAM mRNA and protein in the myotubes, as well as phosphorylation of AMPK and ACC. Furthermore, LF extract and betaine significantly increased the mitochondrial protein contents, as the TFAM and NRF-1 expressions were increased. LF extract and betaine also significantly increased the glucose uptake and ATP contents in the myotubes. The LF extract contained 3.18% betaine was quantitated by HPLC. LF extract and betaine enhanced muscle differentiation and energy metabolism through the up-regulation of mitochondrial biogenesis-regulating factors, suggesting that LF extract and betaine can help to prevent the dysfunction of skeletal muscle.