9 results on '"J. Grobler"'
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2. PP 1.14 – 00094 Development of an immunocytochemistry assay to quantify the translationally active HIV reservoir
- Author
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G. Wu, R. Walters, Y. Li, J. Kristoff, C. Cheney, J. Maxwell, Q. Huang, T. Diamond, P. Zuck, C. Balibar, J. Grobler, B. Howell, and L. Sardo
- Subjects
Microbiology ,QR1-502 ,Public aspects of medicine ,RA1-1270 - Published
- 2022
- Full Text
- View/download PDF
3. Biodiesel production potential of an indigenous South African microalga, Acutodesmus bajacalifornicus
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J. Grobler, K.G. Harding, M. Smit, S. Ramchuran, P. Durand, and M. Low
- Subjects
Acutodesmus bajacalifornicus ,biodiesel ,FAME-GC ,microalgae ,South Africa ,Science - Abstract
In this study, a South African indigenous microalga Acutodesmus bajacalifornicus was evaluated in different cultivation media. Eleven potential cultivation media were identified and tested on A. bajacalifornicus, a potential source for biodiesel production. A. bajacalifornicus had the highest growth rate in the JG medium (in-house formulation), with a competitive average specific growth rate of 0.47 d−1. The highest biomass productivity was in the Hase medium, but with relatively low productivity of 53.1 mg.L−1.d−1.Analysis of the biomass lipid content and profile of each cell culture, using fatty acid methyl ester (FAME) gas chromatography (GC) showed that the lipid content varied between cultivation media, with minimum lipid content of 20% (w/w) and an average close to 47% (w/w). Utilising biodiesel property predictive formulas, and the lipid profiles obtained in this study, it was possible to predict the properties of biodiesel that could be generated from A. bajacalifornicus. It was found that biomass from eight of the media adhered to South African summer grade biodiesel standards.As such, A. bajacalifornicus is a potential candidate for microalgal biodiesel production in South Africa. However, the bioenergy yield rate would need to be improved to have a similar attractiveness to other studies.
- Published
- 2021
- Full Text
- View/download PDF
4. Biodiesel production potential of an indigenous South African microalga, Acutodesmus bajacalifornicus
- Author
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S. Ramchuran, J. Grobler, P. Durand, Kevin G. Harding, Michelle Low, and M. Smit
- Subjects
Biodiesel ,Multidisciplinary ,Acutodesmus bajacalifornicus ,FAME-GC ,microalgae ,Science ,Biomass ,food and beverages ,biodiesel ,complex mixtures ,chemistry.chemical_compound ,South Africa ,Productivity (ecology) ,chemistry ,Bioenergy ,Lipid content ,Biodiesel production ,Acutodesmus ,Environmental science ,Food science ,Fatty acid methyl ester - Abstract
In this study, a South African indigenous microalga Acutodesmus bajacalifornicus was evaluated in different cultivation media. Eleven potential cultivation media were identified and tested on A. bajacalifornicus, a potential source for biodiesel production. A. bajacalifornicus had the highest growth rate in the JG medium (in-house formulation), with a competitive average specific growth rate of 0.47 d−1. The highest biomass productivity was in the Hase medium, but with relatively low productivity of 53.1 mg.L−1.d−1. Analysis of the biomass lipid content and profile of each cell culture, using fatty acid methyl ester (FAME) gas chromatography (GC) showed that the lipid content varied between cultivation media, with minimum lipid content of 20% (w/w) and an average close to 47% (w/w). Utilising biodiesel property predictive formulas, and the lipid profiles obtained in this study, it was possible to predict the properties of biodiesel that could be generated from A. bajacalifornicus. It was found that biomass from eight of the media adhered to South African summer grade biodiesel standards. As such, A. bajacalifornicus is a potential candidate for microalgal biodiesel production in South Africa. However, the bioenergy yield rate would need to be improved to have a similar attractiveness to other studies.
- Published
- 2021
5. Life cycle inventory of bitumen in South Africa
- Author
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Sheldon A. Blaauw, Louis J. Grobler, and James Maina
- Subjects
Protocol (science) ,Pollution ,business.industry ,Mechanical Engineering ,media_common.quotation_subject ,Supply chain ,Environmental resource management ,lcsh:TA1001-1280 ,Aerospace Engineering ,Target audience ,Life cycle inventory ,Life cycle assessment ,Pavement sustainability ,Data quality ,Carbon dioxide equivalent ,Automotive Engineering ,Bitumen ,Economic impact analysis ,lcsh:Transportation engineering ,Safety, Risk, Reliability and Quality ,business ,Function (engineering) ,Life-cycle assessment ,Civil and Structural Engineering ,media_common - Abstract
Road pavements are constructed of bulk raw materials such as aggregate, cement, bitumen and water. The environmental, social, and economic impacts of the materials and processes should be quantified and monitored towards the optimisation of pavement design. At present, no such protocol is in place in South Africa. This paper proposes a framework for the development of a pavement life cycle assessment model, starting by documenting the life cycle inventory for bitumen, one of the leading environmental and social burdensome materials used for pavement development. This inventory acts as the first building block in the development of a life cycle assessment model by evaluating and delineating primary flows (inputs of materials and energy and outputs of pollution) related to the supply chain of bitumen in South Africa. The primary flows are represented by indicators which measure their quantitative impacts. The inventory provides impact category indicators for environmental and social related emissions, energy- and water-use and currently excludes other indicators such as emissions to water, waste generation, jobs creation and economic transformation, amongst others. These indicators are omitted due to lack of quality data at present and difficulty in the quantification of impacts, but recognition is given to their relevancy and importance. The approach is envisioned to function as the first version of a living protocol that will be improved on through further research. Although the primary target audience is South African road authorities, the approach can be adapted for use in any country.
- Published
- 2020
6. Benders decomposition of the passive optical network design problem
- Author
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Magdalena J. Grobler, Melvin Ferreira, SE Terblanche, S. P. van Loggerenberg, 12791911 - Grobler, Magdalena Johanna, 20289278 - Van Loggerenberg, Samuel Pieter, 13041274 - Ferreira, Melvin, and 10794549 - Terblanche, Stephanus Esias
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Mathematical optimization ,021103 operations research ,Applied Mathematics ,Benders decomposition ,Column generation ,0211 other engineering and technologies ,Integer programming ,0102 computer and information sciences ,02 engineering and technology ,01 natural sciences ,Passive optical network ,Facility location problem ,Network planning and design ,010201 computation theory & mathematics ,Software deployment ,Path (graph theory) ,Discrete Mathematics and Combinatorics ,Relaxation (approximation) ,Network design ,Mathematics - Abstract
Similar to the connected facility location problem, the passive optical network design problem requires the search for a subset of deployed distribution points ( splitters ) as well as an allocation of demand points ( optical network units ) to minimise deployment cost. In this paper we decompose a path-based relaxation of the problem using Benders as well as column generation and analyse strengthening cuts for the resulting master. Computational results for this approach are then illustrated.
- Published
- 2016
7. Quadratic variation of martingales in Riesz spaces
- Author
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J. J. Grobler, Valeria Marraffa, Coenraad C.A. Labuschagne, Grobler,J, Labuschagne,C, and Marraffa,V.
- Subjects
Discrete mathematics ,Pure mathematics ,Riesz potential ,Riesz representation theorem ,Applied Mathematics ,martingale ,Riesz space ,vector lattice ,Quadratic variation ,quadratic variation ,M. Riesz extension theorem ,Settore MAT/05 - Analisi Matematica ,Austin’s theorem, Martingale, Measure-free stochastic processes, Quadratic variation, Riesz space, Vector lattice ,measure-free stochastic processes ,Austinʼs theorem ,Martingale (probability theory) ,Analysis ,Mathematics - Abstract
We derive quadratic variation inequalities for discrete-time martingales, sub- and supermartingales in the measure-free setting of Riesz spaces. Our main result is a Riesz space analogue of Austinʼs sample function theorem, on convergence of the quadratic variation processes of martingales http://www.journals.elsevier.com/journal-of-mathematical-analysis-and-applications/ http://dx.doi.org/10.1016/j.jmaa.2013.08.037 National Research Foundation of South Africa (Grant specific unique reference number (UID) 85672) and by GNAMPA of Italy (U 2012/000574 20/07/2012 and U 2012/000388 09/05/2012)
- Published
- 2014
8. Social Life Cycle Inventory for Pavements – A Case Study of South Africa
- Author
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Sheldon A. Blaauw, James W. Maina, and Louis J. Grobler
- Subjects
Social life cycle assessment ,Pavement sustainability ,Social sustainability ,Indicators ,Transportation engineering ,TA1001-1280 - Abstract
Social consideration, assessed using a social life cycle assessment (S-LCA), is a relatively new concept that has yet to be standardised. Attempts at measuring social sustainability in pavement management have been made in South Africa, using social impact assessments (SIAs) and empowerment impact assessment models (EmpIAs). However, these models only focus on certain social considerations and do not incorporate holistic social sustainability in evaluations. The first response to these challenges is to identify and secure universal acceptance for indicators which measure social impacts of pavements and the broader impacts of technical and managerial choices in pavement infrastructure provision. These indicators may then be used to develop a situational- and location-specific social life cycle inventory (S-LCI).This study starts by identifying key social indicators in pavement infrastructure provision and proposes a framework for an S-LCA. Potential indicators were sourced from a large database, focusing on indicators most aligned with social sustainability. Indicators were assessed and scored using an adapted methodology and refinement was conducted through sensitivity analyses employing Bayesian-based Monte Carlo simulations. By allowing weightings and scores of sub-goals and criteria to be changed, the results reflect the importance of inputs from local stakeholders on the impacts of sustainability. A worked example is provided to demonstrate its implementation.The framework is envisioned to function as the first version of a living protocol that will be improved on through further research. Although the primary target audience is South African road authorities, the approach can be adapted for use in any country.
- Published
- 2021
- Full Text
- View/download PDF
9. Dual HIV-1 infection associated with rapid disease progression.
- Author
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Gottlieb GS, Nickle DC, Jensen MA, Wong KG, Grobler J, Li F, Liu SL, Rademeyer C, Learn GH, Karim SS, Williamson C, Corey L, Margolick JB, and Mullins JI
- Subjects
- Acquired Immunodeficiency Syndrome blood, Acquired Immunodeficiency Syndrome virology, Adult, Branched DNA Signal Amplification Assay, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Disease Progression, Female, HIV Infections blood, HIV Infections virology, HIV-1 classification, HIV-1 immunology, Humans, Male, RNA, Viral blood, RNA, Viral immunology, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Superinfection diagnosis, Superinfection immunology, Superinfection virology, Viral Load, Acquired Immunodeficiency Syndrome diagnosis, HIV Infections diagnosis, HIV-1 isolation & purification
- Abstract
Infection with two strains of HIV-1 has implications for understanding HIV transmission and vaccine development; however, frequency and pathogenic consequences of dual infection are unknown. We assessed 64 patients for dual infection with heteroduplex mobility assay, viral sequencing, and phylogenetic methods. HIV disease outcomes were available in 34 patients. Five of these with AIDS endpoints had dual infection with HIV-1: four were cases of coinfection and one was superinfection. In all five, time from seroconversion to clinical AIDS or to CD4+ T-cell count less than 200 cells per microL was very rapid (<3.4 and <3.1 years, respectively). Our findings should prompt larger studies to assess the effect of dual infection at the population level.
- Published
- 2004
- Full Text
- View/download PDF
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