1. Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces
- Author
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István Bakos, Ádám Vass, Eric S. Muckley, Ilia N. Ivanov, and Zsófia Keresztes
- Subjects
Protein adsorption ,Surface coverage ,Copper underpotential deposition ,Industrial electrochemistry ,TP250-261 ,Chemistry ,QD1-999 - Abstract
We demonstrate the use of copper underpotential deposition (UPD) for measurement of the electrochemically active surface area of a protein-modified gold electrode. The kinetics of adsorption and the subsequent structural rearrangement of β-casein, as a model protein, on the surface of a gold electrode have been followed over time. The protein-free surface area was determined by measuring the charge derived from UPD copper deposition on the non-blocked surface area at different stages of adsorption. The behaviour of β-casein adsorption as a function of surface roughness and the microstructure of the gold electrode, as well as the concentration of the protein solution, are studied using this method.
- Published
- 2021
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