1. Role of NAADP for calcium signaling in the salivary gland.
- Author
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Imbery JF, Iqbal AK, Desai T, and Giovannucci DR
- Subjects
- Acinar Cells pathology, Animals, Calcium Signaling, Cells, Cultured, Humans, Male, Mice, Mice, Inbred C57BL, Microscopy, Confocal, Molecular Targeted Therapy, NADP metabolism, Patch-Clamp Techniques, Xerostomia pathology, Acinar Cells metabolism, NADP analogs & derivatives, Pancreas pathology, Salivary Glands pathology, Xerostomia metabolism
- Abstract
Coordination of intracellular Ca
2+ signaling in parotid acini is crucial for controlling the secretion of primary saliva. Previous work from our lab has demonstrated acidic-organelle Ca2+ release as a participant in agonist-evoked signaling dynamics of the parotid acinar cell. Furthermore, results implicated a potential role for the potent Ca2+ releasing second messenger NAADP in these events. The current study interrogated a direct role of NAADP for Ca2+ signaling in the parotid salivary gland acinar cell. Use of live-cell Ca2+ imaging, patch-clamp methods, and confocal microscopy revealed for the first time NAADP can evoke or enhance Ca2+ dynamics in parotid acini. These results were compared with pancreatic acini, a morphologically similar cell type previously shown to display NAADP-dependent Ca2+ signals. Findings presented here may be relevant in establishing new therapeutic targets for those suffering from xerostomia produced by hypofunctioning salivary glands., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
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