1. Physiological response in E. coli to YdgR overexpression depends on whether the protein has an intact function.
- Author
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Sajid S, Salas LH, Rafiq M, Lund T, Jørgensen MG, Honoré B, Christensen LP, Hansen PR, Franzyk H, Mirza O, and Prabhala BK
- Subjects
- Animals, Proteomics, Membrane Transport Proteins metabolism, Carrier Proteins metabolism, Recombinant Proteins metabolism, Mammals metabolism, Escherichia coli metabolism, Escherichia coli Proteins metabolism
- Abstract
Membrane transport proteins are essential for the transport of a wide variety of molecules across the cell membrane to maintain cellular homeostasis. Generally, these transport proteins can be overexpressed in a suitable host (bacteria, yeast, or mammalian cells), and it is well documented that overexpression of membrane proteins alters the global metabolomic and proteomic profiles of the host cells. In the present study, we investigated the physiological consequences of overexpression of a membrane transport protein YdgR that belongs to the POT/PTR family from E. coli by using the lab strain BL21 (DE3)pLysS in its functional and attenuated mutant YdgR-E33Q. We found significant differences between the omics (metabolomics and proteomics) profiles of the cells expressing functional YdgR as compared to cells expressing attenuated YdgR, e.g., upregulation of several uncharacterized y-proteins and enzymes involved in the metabolism of peptides and amino acids. Furthermore, molecular network analysis suggested a relatively higher presence of proline-containing tripeptides in cells expressing functional YdgR. We envisage that an in-depth investigation of physiological alterations due to protein over-expression may be used for the deorphanization of the y-gene transportome., Competing Interests: Declaration of competing interest All authors declare no conflict of interest., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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