1. Modulation of renal ATPase activities by cyclic AMP.
- Author
-
Giesen EM, Imbs JL, Grima M, Schmidt M, and Schwartz J
- Subjects
- 1-Methyl-3-isobutylxanthine pharmacology, Adenosine Triphosphatases metabolism, Animals, Bucladesine pharmacology, Ca(2+) Mg(2+)-ATPase, Colforsin, Diterpenes pharmacology, Isoproterenol pharmacology, Kidney Cortex enzymology, Kidney Medulla enzymology, Kinetics, Male, Rats, Rats, Inbred Strains, Cyclic AMP pharmacology, Kidney enzymology, Sodium-Potassium-Exchanging ATPase antagonists & inhibitors
- Abstract
A possible link between cellular cyclic AMP content and Na+K+ATPase activity was investigated in homogenates of rat kidney. Enzyme kinetics of Mg2+ and Na+K+ATPase were run in the presence of cyclic AMP, dibutyryl cAMP and compounds expected to elevate cyclic AMP levels such as forskolin, a potent adenylate cyclase activator, IBMX, an inhibitor of phosphodiesterases, and the beta-agonist isoproterenol. Medullary Na+K+ATPase is strongly inhibited by cyclic AMP whereas cortical Na+K+ATPase was stimulated in the same conditions. The correlation between ATPase activity and cellular cyclic AMP content supports the concept of a possible regulation of the enzyme by cyclic AMP.
- Published
- 1984
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