1. Tissue Distribution of Radiolabeled Anti-CEA Monoclonal Antibodies in Man
- Author
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E. Cecconato, L. Fusani, Giuliano Mariani, N. Mazzuca, G. Deleide, L. Callegaro, N Molea, Romano Bianchi, M. Dovis, and G.L. Buraggi
- Subjects
medicine.drug_class ,Chemistry ,Cancer ,Spleen ,Initial activity ,medicine.disease ,Monoclonal antibody ,Molecular biology ,Immunoscintigraphy ,medicine.anatomical_structure ,Immunology ,medicine ,Tissue distribution ,Clone (B-cell biology) - Abstract
Anti-CEA monoclonal antibodies (IgG1) from hybridoma F023C5 were labeled with I; I-F(ab’)2 fragments (Fabs) were also prepared, after pepsin-digestion of IgG and double chromatography purification. Labeling was done by the iodogen method (specific activities 13 to 19 mCi/mg). 131I-IgG and 131I-Fab were separately injected each into 4 patients not affected by a CEA-secreting cancer. Plasma disappearances, whole-body imaging, and liver and spleen uptakes were evaluated at daily intervals until 6 days after injection. Following similar early kinetics (about 45% of initial activity in plasma at 24 hours), Fab showed a significantly faster decay rate than IgG: terminal T½ 50.3 ± 6.2 hours versus 88.0 ± 11.7 hours (P
- Published
- 1985
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