1. Preserved immunogenicity of an inactivated vaccine based on foot-and-mouth disease virus particles with improved stability
- Author
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Francisco Sobrino, Kenneth C. McCullough, Miguel A. Martín-Acebes, Artur Summerfield, Flavia Caridi, Ángela Vázquez-Calvo, and Belén Borrego
- Subjects
0301 basic medicine ,Models, Molecular ,Mutant Antibody response ,Swine ,animal diseases ,viruses ,Mutant ,Antibodies, Viral ,Microbiology ,Virus ,03 medical and health sciences ,Improved stability ,Animals ,610 Medicine & health ,Pathogen ,Swine Diseases ,630 Agriculture ,General Veterinary ,biology ,Foot-and-mouth disease virus ,Immunogenicity ,Virion ,virus diseases ,Viral Vaccines ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Virology ,Antibodies, Neutralizing ,030104 developmental biology ,Capsid ,Amino Acid Substitution ,Vaccines, Inactivated ,Foot-and-Mouth Disease ,Inactivated vaccine ,Mutation ,biology.protein ,Capsid Proteins ,Antibody ,Vaccine - Abstract
Foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects important livestock species. Vaccines based on inactivated FMDV virions provide a useful tool for the control of this pathogen. However, long term storage at 4 °C (the temperature for vaccine storage) or ruptures of the cold chain, provoke the dissociation of virions, reducing the immunogenicity of the vaccine. An FMDV mutant carrying amino acid replacements VP1 N17D and VP2 H145Y isolated previously rendered virions with increased resistance to dissociation at 4 °C. We have evaluated the immunogenicity in swine (a natural FMDV host) of a chemically inactivated vaccine based on this mutant. The presence of these amino acid substitutions did not compromise the immunological potential, including its ability to elicit neutralizing antibodies. These results support the feasibility of this kind of mutants with increased capsid stability as suitable viruses for producing improved FMDV vaccines. © 2017 Elsevier B.V.
- Published
- 2017