6 results on '"Fernando Ugarte"'
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2. Progressive Chromatin Condensation and H3K9 Methylation Regulate the Differentiation of Embryonic and Hematopoietic Stem Cells
- Author
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Fernando Ugarte, Rebekah Sousae, Bertrand Cinquin, Eric W. Martin, Jana Krietsch, Gabriela Sanchez, Margaux Inman, Herman Tsang, Matthew Warr, Emmanuelle Passegué, Carolyn A. Larabell, and E. Camilla Forsberg
- Subjects
Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Epigenetic regulation serves as the basis for stem cell differentiation into distinct cell types, but it is unclear how global epigenetic changes are regulated during this process. Here, we tested the hypothesis that global chromatin organization affects the lineage potential of stem cells and that manipulation of chromatin dynamics influences stem cell function. Using nuclease sensitivity assays, we found a progressive decrease in chromatin digestion among pluripotent embryonic stem cells (ESCs), multipotent hematopoietic stem cells (HSCs), and mature hematopoietic cells. Quantitative high-resolution microscopy revealed that ESCs contain significantly more euchromatin than HSCs, with a further reduction in mature cells. Increased cellular maturation also led to heterochromatin localization to the nuclear periphery. Functionally, prevention of heterochromatin formation by inhibition of the histone methyltransferase G9A resulted in delayed HSC differentiation. Our results demonstrate global chromatin rearrangements during stem cell differentiation and that heterochromatin formation by H3K9 methylation regulates HSC differentiation.
- Published
- 2015
- Full Text
- View/download PDF
3. Marine Mammals of the Greenland Seas
- Author
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Aqqalu Rosing-Asvid, Fernando Ugarte, Mads Peter Heide-Jørgensen, and Kristin L. Laidre
- Subjects
geography ,geography.geographical_feature_category ,Oceanography ,biology ,Whale ,Effects of global warming ,biology.animal ,Sea ice ,Conservation status ,Subsistence agriculture - Abstract
This article describes the relationship between sea ice and marine mammals around Greenland, the possible effects of climate change and the importance of subsistence hunting in modern Greenland. We also summarize the biology, conservation status and utilization of the polar bear, walrus, seal and whale species of Greenland.
- Published
- 2020
- Full Text
- View/download PDF
4. Treatment of SHIV-infected, ART-suppressed rhesus macaques with bispecific HIVxCD3 DART® molecules
- Author
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Soha Moltagh, Glareh Azadi, Shirley Ma, Julie Strizki, Bonnie J. Howell, Ming-Tain Lai, Don Graham, Jeffery Nordstrom, Daria J. Hazuda, Meiqing Lu, Carolyn McHale, Romina Riener, Daniel M. Gorman, Fernando Ugarte, Yaoli Song, Marc Bailly, Wendy M. Blumenschein, SuChun Hseih, and Yanyan Zheng
- Subjects
Dart ,Epidemiology ,Chemistry ,Immunology ,Public Health, Environmental and Occupational Health ,Virology ,Microbiology ,QR1-502 ,Infectious Diseases ,Public aspects of medicine ,RA1-1270 ,computer ,computer.programming_language - Published
- 2019
5. Contributors
- Author
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Peter Aastrup, Mohamed M. Abdel-Daim, Jaime A. Aburto, Alicia Acuña, Moisés A. Aguilera, Jahson Alemu, Lotfi Aleya, L. Álvarez-Filip, Sergey V. Alyomov, Rachid Amara, David Amouroux, Pierre Anschutz, Andrés H. Arias, Christos Arvanitidis, Jerald S. Ault, Bolaji Benard Babatunde, Vicente Barros, María Cielo Bazterrica, Béchir Béjaoui, Trine Bekkby, Jacek Bełdowski, Rodolfo C. Bennett, Agnieszka Beszczyńska-Möller, Donna Marie Bilkovic, Ferdinando Boero, David Boertmann, Angel Borja, Alejandro Bortolus, Sandra E. Botté, Luis Bravo, Bernardo R. Broitman, Ernesto Brugnoli, Brenda Burd, JoAnn M. Burkholder, Lawrence B. Cahoon, Sara Calabrese, Yiping Cao, Patricia G. Cardoso, Norving J.T. Cardoza, Xavier Chiappa-Carrara, Brindusa Cristina Chiotoroiu, Tom Christensen, Jorge O. Codignotto, Sarah Cook, Roberto Danovaro, Jean-Claude Dauvin, Antonio M. de Frias Martins, Silvia G. De Marco, Juan Domingo Delgado, Stacy L. Deppeler, Amel Dhib, Mamadou Diop, Cheikh Diop, Marina Dolbeth, Muhammet Duman, Monia El Bour, Rym Ennouri, Cecilia Enríquez, Hüsnü Eronat, Bettina Fach, Melisa D. Fernández Severini, Mouna Fertouna-Bellekhal, Sandra Fiori, James W. Fourqurean, Helene Frigstad, Janne Fritt-Rasmussen, François Galgani, Rafael A. García, R. Garza-Pérez, María Andrea Gavio, Carlos F. Gaymer, Stefan Gelcich, Marcela S. Gerpe, Ferdinand Dumbari Giadom, Erica Giarratano, Mónica Noemí Gil, Judith Gobin, Moncho Gómez-Gesteira, Gongora María Eva Góngora, Shannon Gore, Norman Green, Amy E. Grogan, Valeria A. Guinder, Juan Manuel Gutiérrez, Anders G. Hagen, Christopher Harman, Ioannis Hatzianestis, Kirk J. Havens, Rasmus Hedeholm, Mohamed-Amine Helali, Carl H. Hershner, Kieth Holmes, Jennifer Jackson, Stephen C. Jameson, Kostas Kapiris, Øyvind Kaste, Monika Kędra, Inès Khedhri, Ruy K.P. Kikuchi, Ferah Kocak, Filiz Kucuksezgin, Karol Kuliński, Noelia La Colla, Zelinda M.A.N. Leão, Diego Lirman, Alan Logan, Boris A. López, María Celeste López Abbate, Jerome J. Lorenz, Gustavo Lovrich, Michael A. Mallin, Panagiota Maragou, Jorge E. Marcovecchio, Marino-Tapia Ismael, Maria Virgínia Alves Martins, Karen McLaughlin, Flemming Merkel, Molly M. Mitchell, Azad Mohammed, Terry Mohammed, Vivian Montecino, Alejandro J. Monti, Shelly Moore, Adele K. Morrison, Brian Morton, Anders Mosbech, Pablo Muniz, Andrew Myers, Maite Narvarte, Ana L. Oliva, Marília D.M. Oliveira, Tatyana S. Osadchaya, Achref Othmani, Baghdad Ouddane, Walid Oueslati, Eivind Oug, Panayotis Panayotidis, Vassilis P. Papadopoulos, Vanesa Papiol, Marcela Pascual, Aníbal Pauchard, Alexandra Pavlidou, Giorgos Paximadis, Idil Pazi, Janusz Pempkowiak, Victor Quintino, Nancy N. Rabalais, Marcel Ramos, Miroslav Rangelov, Tania Raymond, Paulo Relvas, Cristóbal Reyes-Hernández, Rodrigo Riera, Frank Rigét, R. Rioja-Nieto, Andrés L. Rivas, Diego H. Rodríguez, José A. Rutllant, Claudio A. Sáez, Dimitris Sakellariou, Baris Salihoglu, Maria Salomidi, Denise M. Sanger, Rui Santos, Ricardo Delfino Schenke, Kenneth Schiff, Kathleen Sullivan Sealey, Alexandra Silva, Nomiki Simboura, Struan R. Smith, Erik Smith, Ronaldo Sousa, Carla V. Spetter, Jonathan S. Stark, Kara Stevens, Beata Szymczycha, Alicia Tagliorette, Martin Thiel, Richard Thomson, Nadezhda Todorova, Tolga Gonul, Lamia Trabelsi, Hilde Trannum, Souad Turki, R. Eugene Turner, Fernando Ugarte, María C. Uyarra, Luis Valdés, Nelson Valdivia, Vasil Vasilev, Natalia Venturini, Tammy Warren, Susse Wegeberg, Stephanie White, Kathleen Wood, Stuart P. Wynne, Cintia Yamashita, Noureddine Zaaboub, Nenibarini Zabbey, Agata Zaborska, Sergia Zalba, and Boutheina Ziadi
- Published
- 2019
- Full Text
- View/download PDF
6. Progressive Chromatin Condensation and H3K9 Methylation Regulate the Differentiation of Embryonic and Hematopoietic Stem Cells
- Author
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Eric W. Martin, Rebekah Sousae, Herman Tsang, Bertrand Cinquin, Margaux Inman, Emmanuelle Passegué, Jana Krietsch, Carolyn A. Larabell, Fernando Ugarte, Gabriela Sanchez, E. Camilla Forsberg, and Matthew R. Warr
- Subjects
Euchromatin ,Cellular differentiation ,Embryoid body ,Biology ,Biochemistry ,Methylation ,Article ,Histones ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Heterochromatin ,Genetics ,Animals ,lcsh:QH301-705.5 ,Embryonic Stem Cells ,030304 developmental biology ,0303 health sciences ,lcsh:R5-920 ,Cell Differentiation ,Cell Biology ,Histone-Lysine N-Methyltransferase ,Chromatin Assembly and Disassembly ,Hematopoietic Stem Cells ,Embryonic stem cell ,Chromatin ,Cell biology ,Endothelial stem cell ,Mice, Inbred C57BL ,lcsh:Biology (General) ,Histone methyltransferase ,Histone Methyltransferases ,Stem cell ,lcsh:Medicine (General) ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Summary Epigenetic regulation serves as the basis for stem cell differentiation into distinct cell types, but it is unclear how global epigenetic changes are regulated during this process. Here, we tested the hypothesis that global chromatin organization affects the lineage potential of stem cells and that manipulation of chromatin dynamics influences stem cell function. Using nuclease sensitivity assays, we found a progressive decrease in chromatin digestion among pluripotent embryonic stem cells (ESCs), multipotent hematopoietic stem cells (HSCs), and mature hematopoietic cells. Quantitative high-resolution microscopy revealed that ESCs contain significantly more euchromatin than HSCs, with a further reduction in mature cells. Increased cellular maturation also led to heterochromatin localization to the nuclear periphery. Functionally, prevention of heterochromatin formation by inhibition of the histone methyltransferase G9A resulted in delayed HSC differentiation. Our results demonstrate global chromatin rearrangements during stem cell differentiation and that heterochromatin formation by H3K9 methylation regulates HSC differentiation., Graphical Abstract, Highlights • Nuclease sensitivity decreases progressively with stem cell differentiation • Distinct chromatin architecture is apparent in cells of different lineage potential • Stem cell differentiation leads to perinuclear heterochromatin localization • G9a-mediated heterochromatin formation facilitates stem cell differentiation, Forsberg and colleagues use a variety of approaches to investigate global chromatin structure and architecture in pluripotent, multipotent, and differentiated cells. They show that progressive changes in nuclease sensitivity, chromatin condensation, and heterochromatin localization correlate with the lineage potential of embryonic and adult stem cells and committed cells. Functionally, inhibition of heterochromatin formation delayed hematopoietic stem cell differentiation. These data provide new insights on the epigenetic regulation of stem cell lineage potential and differentiation.
- Published
- 2015
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