1. Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells
- Author
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Xiangwei Wang, Liqun Yang, Jeong-Hyeon Choi, Eiko Kitamura, Chang-Sheng Chang, Jane Ding, Eun J. Lee, Hongjuan Cui, and Han-Fei Ding
- Subjects
Neuroblastoma ,differentiation ,HOXC9 ,Genetics ,QH426-470 - Abstract
Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in detail the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication [1].
- Published
- 2014
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