16 results on '"Elena Sánchez-López"'
Search Results
2. The two enantiomers of 2-hydroxyglutarate differentially regulate cytotoxic T cell function
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Iosifina P. Foskolou, Pedro P. Cunha, Elena Sánchez-López, Eleanor A. Minogue, Benoît P. Nicolet, Aurélie Guislain, Christian Jorgensen, Sarantos Kostidis, Nordin D. Zandhuis, Laura Barbieri, David Bargiela, Demitris Nathanael, Petros A. Tyrakis, Asis Palazon, Martin Giera, Monika C. Wolkers, and Randall S. Johnson
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CP: Immunology ,Biology (General) ,QH301-705.5 - Abstract
Summary: 2-Hydroxyglutarate (2HG) is a byproduct of the tricarboxylic acid (TCA) cycle and is readily detected in the tissues of healthy individuals. 2HG is found in two enantiomeric forms: S-2HG and R-2HG. Here, we investigate the differential roles of these two enantiomers in cluster of differentiation (CD)8+ T cell biology, where we find they have highly divergent effects on proliferation, differentiation, and T cell function. We show here an analysis of structural determinants that likely underlie these differential effects on specific α-ketoglutarate (αKG)-dependent enzymes. Treatment of CD8+ T cells with exogenous S-2HG, but not R-2HG, increased CD8+ T cell fitness in vivo and enhanced anti-tumor activity. These data show that S-2HG and R-2HG should be considered as two distinct and important actors in the regulation of T cell function.
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- 2023
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3. Determination of l-norvaline and l-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column
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Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, and María Luisa Marina
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Therapeutics. Pharmacology ,RM1-950 - Abstract
An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested, and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them. Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity, precision, accuracy, limits of detection (LOD) and quantitation (LOQ) showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements. LOD and LOQ values were 9.3 and 31 μM for norvaline enantiomers and 7.5 and 25 μM for tryptophan enantiomers, respectively. The contents of d-norvaline and d-tryptophan were below their respective LODs in all the analyzed samples. Quantitation of l-tryptophan and l-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of l-norvaline, contrary to the label indication. Keywords: 9-Fluorenylmethoxycarbonyl, Amino acids, Chiral separation, Nano-LC, Quinidine-silica hybrid monolithic column
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- 2020
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4. Single-step fabrication of a teicoplanin functionalized organic-silica hybrid monolith for enantioseparation by nano-liquid chromatography
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Dongsheng Xu, Huikai Shao, Liang Lai, Elena Sánchez-López, María Luisa Marina, and Zhengjin Jiang
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Teicoplanin organic-silica hybrid monolith ,Enantioseparation ,Nano-LC ,Amino alcohols and amino acids ,Mandelic acids ,Analytical chemistry ,QD71-142 - Abstract
A novel teicoplanin functionalized organic-silica hybrid chiral monolithic column was prepared by a facile single-step approach. The conditions for the preparation of the organic-silica hybrid chiral monolithic column were systematically optimized, including the amount of poly (ethylene glycol) (PEG) and urea, the tetramethyl orthosilicate (TMOS)/3-(trimethoxysilyl)-propylmethacrylate (γ-MAPS) ratio (v/v), the content of teicoplanin-2-isocyanatoethyl methacrylate (Tei-ICNEML) monomer, and the reaction temperature and time. The optimum column exhibited satisfactory efficiency, permeability and stability. In order to obtain satisfactory enantioseparation performance, reversed phase (RPM) and polar organic phase (POM) modes were employed, and different experimental conditions were optimized, such as buffer concentration and pH, amount of the organic solvent, concentration and ratio of TEA/HAc, and MeOH/ACN ratio. The stability of the Tei-ICNEML organic-silica hybrid monolithic column was also investigated in both POM and RPM separation modes. Then, this novel monolithic column was successfully applied to achieve the enantioseparation of amino alcohols, N-derivatized amino acids and mandelic acids. 15 out of 20 amino alcohols were baseline separated with good column efficiency in the POM mode, and 3 out of 5 mandelic acids and 5 out of 6 N-derivatized amino acids were baseline separated in the RPM mode.
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- 2021
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5. Dexibuprofen prevents neurodegeneration and cognitive decline in APPswe/PS1dE9 through multiple signaling pathways
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Miren Ettcheto, Elena Sánchez-López, Laura Pons, Oriol Busquets, Jordi Olloquequi, Carlos Beas-Zarate, Merce Pallas, Maria Luisa García, Carme Auladell, Jaume Folch, and Antoni Camins
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APPSwe/PS1dE9 ,Dexibuprofen ,Insulin receptor ,Mitochondria ,Hippocampus ,TAU ,Memory impairment ,Alzheimer's disease ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
The aim of the present study is to elucidate the neuronal pathways associated to NSAIDs causing a reduction of the risk and progression of Alzheimer's disease. The research was developed administering the active enantiomer of ibuprofen, dexibuprofen (DXI), in order to reduce associated gastric toxicity. DXI was administered from three to six-month-old female APPswe/PS1dE9 mice as a model of familial Alzheimer's disease. DXI treatment reduced the activation of glial cells and the cytokine release involved in the neurodegenerative process, especially TNFα. Moreover, DXI reduced soluble β-amyloid (Aβ1-42) plaque deposition by decreasing APP, BACE1 and facilitating Aβ degradation by enhancing insulin-degrading enzyme. DXI also decreased TAU hyperphosphorylation inhibiting c-Abl/CABLES/p-CDK5 activation signal pathway and prevented spatial learning and memory impairment in transgenic mice. Therefore, chronic DXI treatment could constitute a potential AD-modifying drug, both restoring cognitive functions and reversing multiple brain neuropathological hallmarks.
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- 2017
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6. The role of blood–brain and blood–retinal barriers in drug delivery
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Lorena Bonilla-Vidal, Gerard Esteruelas, Eliana B. Souto, Marta Espina, María Luisa García, and Elena Sánchez López
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- 2023
7. Contributors
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Mukta Agrawal, Junia Akhtar, Amit Alexander, Gulin Amasya, Taruna Arora, Kajal Bagri, Sanya Batheja, Zerrin Sezgin Bayindir, Jayant Singh Bhardwaj, Abhay Bhardwaj, Swapnali Vasant Birajdar, Antonio Camins, Amanda Cano, Akash Chaurasiya, Rusham Das, Rahul Deshmukh, Sunil Kumar Dubey, Marta Espina, Gerard Esteruelas, Miren Ettcheto, Maria Luisa García, Goutam Ghosh, Amit Kumar Goyal, Deepika Grewal, Umesh Gupta, Sanyog Jain, Biswakanth Kar, Oly Katari, Sumeet Katke, Prashant Kesharwani, Sanskruti Santosh Kharavtekar, Vinay Kumar, Shilpa Kumari, Priyanka Kumari, Shobha Kumari, Farhan Mazahir, Manisha Mulchandani, Jayabalan Nirmal, Kanan Panchal, Tushar Kanti Rajwar, Goutam Rath, Shreyasi Ghosh Ray, Upal Roy, Rakesh Kumar Sahoo, Eliana B. Souto, Elena Sánchez-López, Rajeev Taliyan, Awesh K. Yadav, Sheetal Yadav, and Nilufer Yuksel
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- 2023
8. Polymeric nanoparticles as drug delivery systems for dementia
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Gerard Esteruelas, Eliana B. Souto, Amanda Cano, Miren Ettcheto, Marta Espina, Antonio Camins, Maria Luisa García, and Elena Sánchez-López
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Nanomedicine ,Drug delivery ,PLGA ,Dementia ,Alzheimer’s disease ,Polymeric nanoparticles - Abstract
Dementia affects around 50 million people worldwide and it is one of the main causes of disability and dependency. The incidence of dementia is projected to increase rapidly over the next several decades. However, despite these increased number of people suffering from this syndrome, available pharmacological treatments are highly limited. Nanotechnological approaches could constitute the key to deliver drugs effectively to the target site in a prolonged manner and avoiding undesired adverse effects. Polymeric nanoparticles are one of the most promising drug delivery systems, being able to encapsulate a wide variety of compounds. Several studies had been made using nanoparticles, especially for Alzheimer’s disease. This chapter analyses the different types of dementia and provides a critical and global vision of the main polymers used to develop polymeric nanoparticles and their specific applications for dementia. An overview about functionalization strategies employed for the treatment of dementia is also carried out., A.C. aknowledges the support of the Spanish Ministry of Science, Innovation and Universities under the grant Juan de la Cierva (FJC2018-036012-I), and project grant PID2021-122473OA-I00. ESL acknowledges the support of the Programme of Requalification of the Spanish University System.
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- 2023
9. List of contributors
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Ana Beatriz Afonso, Robson Amaral, Luciana N. Andrade, Heloisa Balan Assalin, Reinaldo Gaspar Bastos, Catherine Baugé, Sarwar Beg, Gabriel Gaspar Bíscaro, S. Bonde, Lorena Bonilla-Vidal, P. Boonme, Karim Boumediene, Carolina Caliari-Oliveira, Amanda Cano, Raffaele Capasso, Claudia Carbone, Juliana C. Cardoso (J.C. Cardoso), Marco Vinicius Chaud (Marco V. Chaud), Beatriz Clares, Maria C. Costa, Tiago E. Coutinho, Ana T. Cruz, Classius F. da Silva, Ricardo L.C. de Albuquerque-Júnior, Luciana M. de Hollanda, Bruna Alice G. de Melo (B.A.G. de Melo), Alexandre Leite Rodrigues de Oliveira, Alexandro Barbosa de Souza, J. Dias-Ferreira, Andrey dos Santos, Nelson Durán, Alessandra Durazzo, Piotr Eder, Marta Espina, Gerard Esteruelas, Wagner J. Fávaro, Fabio Rocha Formiga, Inês Formoso, Carla G. França, Patricia Gálvez, María Luisa García, María T. Garrido, Mira Hammad, Mayank Handa, Stephany C. Huber, J. Jovanovic, Marat I. Kamalov, Anðelka Kovačevi, José Fabio S.D. Lana, Krissia Caroline Leme, Daniele M. Lima, Massimo Lucarini, Ângela Cristina Malheiros Luzo (Ângela Cristina M. Luzo), Adriana Oliveira Manfiolli, Patrick Masson, Luis Fernando Mercier Franco, Kailas Kalicharan Moravkar, Joana Nobre, Chandrakantsing Vijaysing Pardeshi, Tatiana N. Pashirova, M. Patel, R. Patel, Jaywant Pawar, Pedro de Alcântara Pessoa Filho, M. Rai, Manuel Ríos, Elena Sánchez López, Elena López, Maria Helena A. Santana (Maria H. Santana), Antonello Santini, Patrícia Severino (P. Severino), Ranjita Shegokar, Andrea A.M. Shimojo, Dilip Waman Shingare, Rahul Shukla, Amélia M. Silva, K.K. Singh, Mukesh Soni, José L. Soriano, Eliana B. Souto, Claudia Herrera Tambeli, M.C. Teixeira, Natalia V. Terekhova, and Aleksandra Zielinska (A. Zielińska)
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- 2023
10. Phenomics of elite heirlooms of peppers (Capsicum annuum L.) from the Spanish centre of diversity: Conventional and high-throughput digital tools towards varietal typification
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Leandro Pereira-Dias, Adrián Rodríguez-Burruezo, Elena Sánchez-López, Santiago Vilanova, and Ana Fita
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0106 biological sciences ,0301 basic medicine ,Capsicum annuum ,Ecotypes ,media_common.quotation_subject ,Protected Designations of Origin ,Library science ,Horticulture ,01 natural sciences ,03 medical and health sciences ,GENETICA ,030104 developmental biology ,Phenomics ,Political science ,Germplasm ,Elite ,IPGRI ,Tomato Analyzer ,Typification ,010606 plant biology & botany ,Diversity (politics) ,media_common - Abstract
[EN] Spain is a relevant secondary centre of diversity for Capsicum annuum peppers, especially for the bell types known as Pimiento Morron or Pimiento de Morro. Thus, a myriad of highly regarded landraces adapted to a wide range of conditions can be found throughout the country, as a result of centuries of farmers breeding. Despite that, these materials lack of proper characterization, of paramount importance for farmers, breeders and germplasm management. In this regard, in addition to internationally accepted conventional descriptors, high-throughput digital phenotyping tools like Tomato Analyzer, a software originally developed to process scanned images of cut tomato fruits and to record a range of morphological parameters, may provide an important help towards exhaustive germplasm characterization. With this aim, 32 conventional and 35 Tomato Analyzer digital traits were used herein to characterize a large collection of C. annuum accessions from all Spanish regions, including Protected Designations of Origin and Protected Geographical Indications, with emphasis on Marron peppers, in order to assess the diversity within Spanish elite germplasm and to test the efficiency of those methods to differentiate varietal types and closely related materials. A considerable amount of variation was found using both conventional and digital traits, even within Marron pepper groups, reflecting the diversity of Spanish peppers in terms of plant and fruit morphology, essential for future breeding programs. Both conventional descriptors and digital parameters were able to distinguish varietal groups. However, on the whole, digital phenotyping was able to discriminate in a more accurate way. Most digital parameters were able to discriminate varietal groups into higher numbers of categories (>= 4) than conventional traits (usually 2-4). In addition, the number of significant pairwise differences among varietal groups was considerably higher for digital parameters than for conventional descriptors, enabling a powerful separation, particularly relevant for closely related groups such as Morron peppers. Likewise, as revealed by Principal Components Analysis, digital phenotyping allowed a more powerful intra-varietal separation compared to conventional descriptors. Finally, a subset of 4 conventional descriptors and 13 Tomato Analyzer traits were identified as the most discriminant to distinguish among closely related C. annuum accessions, explaining 81.81 % of total variance found by Principal Components Analysis. Fruit traits explained the highest percentage of variance for our collection, This work has been financed by INIA projects RTA2014-00041-C02-02 and RF2010-00025-00-00, FEDER fund\s. Authors are also grateful to the different Research Institutions, scientists and breeders, and PDOs and PGIs Regulatory Boards, included on Table 1 for providing part of the materials studied here. Finally, thanks are given to Prof. Jaime Prohens for his kind and useful advice in the statistic interpretation of PCAs and MSc Elena Rosa for her help in the edition of Fig. 1.
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- 2021
11. Improving graph-based detection of singular events for photochemical smog agents
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Javier Gómez-Gómez, Rafael Carmona-Cabezas, João Serrano, Eduardo Gutiérrez de Ravé, Francisco J. Jiménez-Hornero, and Elena Sánchez-López
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Visibility graphs ,Environmental Engineering ,Computer science ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,02 engineering and technology ,010501 environmental sciences ,Photochemistry ,01 natural sciences ,Photochemical smog ,Singularity ,Singularity detection ,Environmental Chemistry ,0105 earth and related environmental sciences ,Air Pollutants ,Smog ,Visibility graph ,Graph based ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Multifractal system ,Degree distribution ,Photochemical Processes ,Pollution ,Graph ,020801 environmental engineering ,Environmental Monitoring - Abstract
Recently, a set of graph-based tools have been introduced for the identification of singular events of O3, NO2 and temperature time series, as well as description of their dynamics. These are based on the use of the Visibility Graphs (VG). In this work, an improvement of the original approach is proposed, being called Upside-Down Visibility Graph (UDVG). It adds the possibility of investigating the singular lowest episodes, instead of the highest. Results confirm the applicability of the new method for describing the multifractal nature of the underlying O3, NO2, and temperature. Asymmetries in the NO2 degree distribution are observed, possibly due to the interaction with different chemicals. Furthermore, a comparison of VG and UDVG has been performed and the outcomes show that they describe opposite subsets of the time series (low and high values) as expected. The combination of the results from the two networks is proposed and evaluated, with the aim of obtaining all the information at once. It turns out to be a more complete tool for singularity detection in photochemical time series, which could be a valuable asset for future research.
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- 2020
12. Neuroscience Applications of Capillary Electrophoretic Methods
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María Luisa Marina and Elena Sánchez-López
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03 medical and health sciences ,0302 clinical medicine ,Capillary electrophoresis ,Computer science ,010401 analytical chemistry ,Injection volume ,01 natural sciences ,Neuroscience ,030217 neurology & neurosurgery ,0104 chemical sciences - Abstract
The outstanding features of capillary electrophoresis (CE) make it a suitable technique to increase understanding in the field of neuroscience. Its low injection volume is of utmost importance in the analysis of samples related to neuroscience. In this chapter, the literature regarding CE applications in neuroscience is described from the perspective of the analytical approach used. Without doubt, microdialysis is the most important temporal-sampling approach for biological systems. The information obtained can be enhanced if microdialysis is coupled online to CE. CE-laser-induced fluorescence is most often used in neuroscience applications, due to its high sensitivity. Single-cell analysis is also possible using CE, opening up new possibilities to study of neurological systems. Future trends are directed at developing more sensitive methods with minimal sample preparation. Despite the scarce use of mass spectrometry (MS) at present, new approaches should take advantage of MS to obtain better selectivity and for the identification of unknown molecules.
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- 2018
13. Contributors
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Alain Beck, Michael C. Breadmore, Lucas P. Bressan, Cyro L.S. Chagas, Wendell K.T. Coltro, Fanny d’Orlyé, José A.F. da Silva, Dosil P. de Jesus, Gerhardus de Jong, Claudia Desiderio, Yannis-Nicolas François, Rabah Gahoual, Ana M. García-Campaña, Jérémie Giorgetti, Wojciech Grochocki, David S. Hage, Maykel Hernández-Mesa, Takeshi Hirokawa, Oliver Höcker, Laurel Jones, Kevin Jooß, Václav Kašička, Ernst Kenndler, Francisco J. Lara, Emmanuelle Leize-Wagner, Shao-Ping Li, Norbert M. Maier, María Luisa Marina, Michal J. Markuszewski, Claudia Martelli, Ivan Mikšík, Roger C. Moreira, David Moreno-González, Christian Neusüß, Ute Pyell, Joselito P. Quirino, Gonzalo Ramírez-García, Jennifer Römer, Elena Sánchez-López, Cari E. Sänger-van de Griend, Kiyohito Shimura, Laura Sola, Sille Štěpánová, Wolfgang Thormann, Laura Trapiella-Alfonso, Ann Van Schepdael, Anne Varenne, Yan Wang, Yun Xue, Chao Yan, Feng-Qing Yang, Chenhua Zhang, Qian Zhang, and Jing Zhao
- Published
- 2018
14. Capillary Electrophoresis: Chiral Separations
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Elena Sánchez-López, María Castro-Puyana, and María Luisa Marina
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Chromatography ,Capillary electrophoresis ,Chemistry - Published
- 2018
15. Protein tyrosine phosphatase 1B (PTP1B) as a potential therapeutic target for neurological disorders
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Jordi Olloquequi, Amanda Cano, Elena Sanchez-López, Marina Carrasco, Ester Verdaguer, Ana Fortuna, Jaume Folch, Mònica Bulló, Carme Auladell, Antoni Camins, and Miren Ettcheto
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PTP1B ,Type 2 diabetes ,Insulin receptor ,Alzheimer’s disease ,Neuroinflammation ,Neurological disorders ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Protein tyrosine phosphatase 1B (PTP1B) is a typical member of the PTP family, considered a direct negative regulator of several receptor and receptor-associated tyrosine kinases. This widely localized enzyme has been involved in the pathophysiology of several diseases. More recently, PTP1B has attracted attention in the field of neuroscience, since its activation in brain cells can lead to schizophrenia-like behaviour deficits, anxiety-like effects, neurodegeneration, neuroinflammation and depression. Conversely, PTP1B inhibition has been shown to prevent microglial activation, thus exerting a potent anti-inflammatory effect and has also shown potential to increase the cognitive process through the stimulation of hippocampal insulin, leptin and BDNF/TrkB receptors. Notwithstanding, most research on the clinical efficacy of targeting PTP1B has been developed in the field of obesity and type 2 diabetes mellitus (TD2M). However, despite the link existing between these metabolic alterations and neurodegeneration, no clinical trials assessing the neurological advantages of PTP1B inhibition have been performed yet. Preclinical studies, though, have provided strong evidence that targeting PTP1B could allow to reach different pathophysiological mechanisms at once. herefore, specific interventions or trials should be designed to modulate PTP1B activity in brain, since it is a promising strategy to decelerate or prevent neurodegeneration in aged individuals, among other neurological diseases. The present paper fails to include all neurological conditions in which PTP1B could have a role; instead, it focuses on those which have been related to metabolic alterations and neurodegenerative processes. Moreover, only preclinical data is discussed, since clinical studies on the potential of PTP1B inhibition for treating neurological diseases are still required.
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- 2022
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16. Physicochemical and biopharmaceutical aspects influencing skin permeation and role of SLN and NLC for skin drug delivery
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Eliana B. Souto, Joana F. Fangueiro, Ana R. Fernandes, Amanda Cano, Elena Sanchez-Lopez, Maria L. Garcia, Patrícia Severino, Maria O. Paganelli, Marco V. Chaud, and Amélia M. Silva
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Dermal application ,Solid lipid nanoparticles ,Nanostructured lipid carriers ,Bioavailability ,Skin permeation ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The skin is a complex and multifunctional organ, in which the static versus dynamic balance is responsible for its constant adaptation to variations in the external environment that is continuously exposed. One of the most important functions of the skin is its ability to act as a protective barrier, against the entry of foreign substances and against the excessive loss of endogenous material. Human skin imposes physical, chemical and biological limitations on all types of permeating agents that can cross the epithelial barrier. For a molecule to be passively permeated through the skin, it must have properties, such as dimensions, molecular weight, pKa and hydrophilic-lipophilic gradient, appropriate to the anatomy and physiology of the skin. These requirements have limited the number of commercially available products for dermal and transdermal administration of drugs. To understand the mechanisms involved in the drug permeation process through the skin, the approach should be multidisciplinary in order to overcome biological and pharmacotechnical barriers. The study of the mechanisms involved in the permeation process, and the ways to control it, can make this route of drug administration cease to be a constant promise and become a reality. In this work, we address the physicochemical and biopharmaceutical aspects encountered in the pathway of drugs through the skin, and the potential added value of using solid lipid nanoparticles (SLN) and nanostructured lipid vectors (NLC) to drug permeation/penetration through this route. The technology and architecture for obtaining lipid nanoparticles are described in detail, namely the composition, production methods and the ability to release pharmacologically active substances, as well as the application of these systems in the vectorization of various pharmacologically active substances for dermal and transdermal applications. The characteristics of these systems in terms of dermal application are addressed, such as biocompatibility, occlusion, hydration, emollience and the penetration of pharmacologically active substances. The advantages of using these systems over conventional formulations are described and explored from a pharmaceutical point of view.
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- 2022
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