1. LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway.
- Author
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Du W, Feng Z, and Sun Q
- Subjects
- Animals, Base Sequence, Binding Sites, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Female, Gene Expression Regulation, Neoplastic, Humans, Mice, Nude, Neoplasm Invasiveness, RNA, Long Noncoding genetics, Up-Regulation, Disease Progression, MicroRNAs metabolism, Neoplasm Proteins metabolism, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology, RNA, Long Noncoding metabolism, Repressor Proteins metabolism, Signal Transduction
- Abstract
Long noncoding RNA (lncRNA) LINC00319 has been reported to promote carcinogenesis of lung cancer and cutaneous squamous cell carcinoma. However, the role and mechanism of LINC00319 in ovarian cancer progression is unclear. In this study, LINC00319 expression was found to be upregulated in ovarian cancer tissues and cell lines. And our evidence showed that LINC00319 could be a potential prognostic biomarker for patients with ovarian cancer. Cell Counting Kit-8 (CCK-8), colony formation and transwell assays indicated that LINC00319 upregulation promoted proliferation, migration and invasion of ovarian cancer cells. Bioinformatics analysis and luciferase reporter assay revealed that LINC00319 worked as the sponge for miR-423-5p. Furthermore, miR-423-5p directly targeted NACC1. qRT-PCR and western blot results demonstrated that LINC00319 upregulates NACC1 expression through inhibiting miR-423-5p in ovarian cancer cells. Moreover, we observed an inverse expression correlation between miR-423-5p and LINC00319 or between miR-423-5p and NACC1 in ovarian cancer tissues. Finally, rescue assay showed that NACC1 restoration rescued the potentials of proliferation, migration and invasion in LINC00319-depleted ovarian cancer cells. In conclusion, our findings demonstrated that LINC00319 promotes ovarian cancer progression through upregulating NACC1 expression by restraining miR-423-5p., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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