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Molecular imprinting has advanced towards synthesizing whole-cell imprints of microorganisms such as bacteria on various sensor surfaces including wire electrodes, quartz crystal microbalances, and microparticles (MPs). We recently introduced cell-imprinted polymers (CIPs) coated on MPs, called CIP-MPs, for bacteria recovery from water. In this paper, we have advanced towards rapid fluorometric Escherichia coli (E. coli) detection by applying fluorescent magnetic CIP-MPs, which were captured by soft ferromagnetic microstructures integrated into a microfluidic channel. The shape of ferromagnetic microstructures was optimized numerically to enhance the magnetophoretic accumulation of CIP-MPs in the microchannel. The device was then fabricated and the flow rate required to enhance bacteria capturing efficiency by CIP-MPs was determined. Decreasing the flow rate reduced the flow-induced drag and increased the interactions between the bacteria and imprinted CIP cavities. Fluorescent imaging of the accumulated CIP-MPs was done before and after bacteria capturing to quantify the changes in the fluorescence intensity as E. coli cells were captured by CIP-MPs in the microchannel. The dose-response curve of the sensor at 0-10 9  CFU/mL bacterial counts was obtained. Using the 3- and 10-sigma methods on the dose-response curve, the limits of detection (LOD) and quantification (LOQ) of the sensor were determined to be 4 × 10 2 and 3 × 10 3  CFU/mL, respectively, within a dynamic range of 10 2 -10 7  CFU/mL. Lastly, the specificity of the CIP-MPbased sensor towards E. coli was tested and confirmed using Sarcina as a non-specific target bacterium. In summary, our developed sensor offers a promising approach for rapid and low-cost detection of bacteria in water and is suitable for development of portable and durable all-polymeric sensors for point-of-need detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

With the COVID-19 pandemic, the threat of infectious diseases to public health and safety has become much more apparent. Viral, bacterial and fungal diseases have led to the loss of millions of lives, especially in the developing world. Diseases caused by airborne viruses like SARS-CoV-2 are difficult to control, as these viruses are easily transmissible and can circulate in the air for hours. To contain outbreaks of viruses such as SARS-CoV-2 and institute targeted precautions, it is important to detect them in air and understand how they infect their targets. Point-of-care (PoC) diagnostics and point-of-need (PoN) detection methods are necessary to rapidly test patient and environmental samples, so precautions can immediately be applied. Traditional benchtop detection methods such as ELISA, PCR and culture are not suitable for PoC and PoN monitoring, because they can take hours to days and require specialized equipment. Microfluidic devices can be made at low cost to perform such assays rapidly and at the PoN. They can also be integrated with air- and liquid-based sampling technologies to capture and analyze viruses from air and body fluids. Here, conventional and microfluidic virus detection methods are reviewed and compared. The use of air sampling devices to capture and concentrate viruses is discussed first, followed by a review of analysis methods such as immunoassays, RT-PCR and isothermal amplification in conventional and microfluidic platforms. This review provides an overview of the capabilities of microfluidics in virus handling and detection, which will be useful to infectious disease researchers, biomedical engineers, and public health agencies., (Copyright © 2021 Elsevier B.V. All rights reserved.)

In this paper, we applied a curved-channel microfluidic device to separate DNA from PCR-inhibitor-containing water and simultaneously wash them into clean water for detection using a portable PCR thermocycler. Environmental DNA (eDNA) sampling has become an effective surveying approach for detecting rare organisms. However, low concentration eDNA molecules may be masked by PCR inhibitors during amplification and detection, increasing the risk of false negatives. Therefore, technologies for on-site DNA separation and washing are urgently needed. Our device consisted of a half-circle microchannel with a DNA-inhibitor sample inlet, a clean buffer inlet, and multiple outlets. By using the flow-induced inertial forces, 10 μm DNA-conjugated microparticles were focused at the inner-wall of the curved microchannel while separation from 1 μm inhibitor-conjugated microparticles and DNA washing were achieved simultaneously with the Dean flow. We achieved singleplex focusing, isolation and washing of 10 μm particles at an efficiency of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, larger particles were washed with an efficiency of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity groups against Atlantic salmon DNA and humic acid (HA), and processing samples of various concentrations in our device, we achieved an effective purification and detection of DNA molecules using the portable PCR thermocycler. Our method significantly decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed device takes a promising step forward in sample preparation towards an integrated device that can be used for simultaneous purification and solution exchange of DNA in point-of-need environmental monitoring applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

One of the significant challenges in bone tissue engineering is the fabrication of highly porous scaffolds with interconnected pores and appropriate mechanical properties. Artificial scaffolds which used in the field of medicine are usually made of single phase of polymer or ceramic. However, composition of these materials can produce the scaffolds with improve mechanical and biological properties.The aim of this study is to synthesize three-dimensional hardystonite-diopside (HT-Dio) porous scaffolds modified by polycaporolacton fumarate coating for low-load-bearing bone tissue engineering applications. The results showed that hardystonite scaffolds with 15 wt. % diopside and 6 w/v % polymer polycaporolacton fumarate (PCLF) had a significant bioactivity. The cell culture and cell attachment assay results revealed the well spreading of BMS cells on the surface of modified scaffolds which indicates the high biocompatibility of this scaffold. The modified scaffolds had a mean pore size, porosity, compressive strength, modules and toughness of 293.47 ± 5.51 μm, 74% ± 1.01, 3.37 ± 0.6 MPa, 151 ± 1.1 MPa and 31.3 ± 0.32 kJ/m 3 , respectively, which are in the appropriate range for spongy bone and hence can be a good candidate for bone tissue engineering applications., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Porous Si-based ceramic scaffolds are widely attracted in biomedical tissue engineering application. Despite the attractive properties of these materials, their weak mechanical properties and high degradability in vitro and in vivo environment can limit their application as biomedical devises. Applying a thin layer of polymer on the surface of porous scaffolds can improve the mechanical properties and control the degradation rate. In this study, we produced new modified scaffolds with polymers coating in order to improved mechanical and biological properties of Si-based ceramics scaffolds. The results showed that applying 6 wt% PCLF polymer on the surface of Bagh-15 wt%Dio scaffolds delayed apatite formation compared to unmodified scaffolds. On the other hand, in the modified scaffolds, apatite formation was observed. The degradation rate of unmodified scaffolds was decreased around 82% after 28 days soaking in PBS solution. Based on the MTT assay and SEM micrographs, the BMS cells were spread and attached well on the surface of the scaffolds, which indicated a good biocompatibility. The results showed that these scaffolds have the potential to be used as a temporary substrate for bone tissue engineering application., (Copyright © 2019 Elsevier B.V. All rights reserved.)