1. Myosins and DYNLL1/LC8 in the honey bee (Apis mellifera L.) brain.
- Author
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Calábria LK, Peixoto PM, Passos Lima AB, Peixoto LG, de Moraes VR, Teixeira RR, Dos Santos CT, E Silva LO, da Silva Mde F, dos Santos AA, Garcia-Cairasco N, Martins AR, Espreafico EM, and Espindola FS
- Subjects
- Adenosine Triphosphate, Animals, Brain metabolism, Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism, Immunohistochemistry, Melitten, N-Methylaspartate, Synaptophysin metabolism, Bees metabolism, Insect Proteins metabolism, Myosins metabolism, SNARE Proteins metabolism
- Abstract
Honey bees have brain structures with specialized and developed systems of communication that account for memory, learning capacity and behavioral organization with a set of genes homologous to vertebrate genes. Many microtubule- and actin-based molecular motors are involved in axonal/dendritic transport. Myosin-Va is present in the honey bee Apis mellifera nervous system of the larvae and adult castes and subcastes. DYNLL1/LC8 and myosin-IIb, -VI and -IXb have also been detected in the adult brain. SNARE proteins, such as CaMKII, clathrin, syntaxin, SNAP25, munc18, synaptophysin and synaptotagmin, are also expressed in the honey bee brain. Honey bee myosin-Va displayed ATP-dependent solubility and was associated with DYNLL1/LC8 and SNARE proteins in the membrane vesicle-enriched fraction. Myosin-Va expression was also decreased after the intracerebral injection of melittin and NMDA. The immunolocalization of myosin-Va and -IV, DYNLL1/LC8, and synaptophysin in mushroom bodies, and optical and antennal lobes was compared with the brain morphology based on Neo-Timm histochemistry and revealed a distinct and punctate distribution. This result suggested that the pattern of localization is associated with neuron function. Therefore, our data indicated that the roles of myosins, DYNLL1/LC8, and SNARE proteins in the nervous and visual systems of honey bees should be further studied under different developmental, caste and behavioral conditions., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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