1. Hybrid Spectral Library Combining DIA-MS Data and a Targeted Virtual Library Substantially Deepens the Proteome Coverage
- Author
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Ronghui Lou, Pan Tang, Kang Ding, Shanshan Li, Cuiping Tian, Yunxia Li, Suwen Zhao, Yaoyang Zhang, and Wenqing Shui
- Subjects
Science - Abstract
Summary: Data-independent acquisition mass spectrometry (DIA-MS) is a powerful technique that enables relatively deep proteomic profiling with superior quantification reproducibility. DIA data mining predominantly relies on a spectral library of sufficient proteome coverage that, in most cases, is built on data-dependent acquisition-based analysis of the same sample. To expand the proteome coverage for a pre-determined protein family, we report herein on the construction of a hybrid spectral library that supplements a DIA experiment-derived library with a protein family-targeted virtual library predicted by deep learning. Leveraging this DIA hybrid library substantially deepens the coverage of three transmembrane protein families (G protein-coupled receptors, ion channels, and transporters) in mouse brain tissues with increases in protein identification of 37%–87% and peptide identification of 58%–161%. Moreover, of the 412 novel GPCR peptides exclusively identified with the DIA hybrid library strategy, 53.6% were validated as present in mouse brain tissues based on orthogonal experimental measurement. : Analytical Chemistry; Biological Sciences; Classification of Proteins; Proteomics Subject Areas: Analytical Chemistry, Biological Sciences, Classification of Proteins, Proteomics
- Published
- 2020
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