1. Differing energy densities with laser 670nm InGaP controls inflammation and collagen reorganization in burns.
- Author
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Maligieri LAO, Neves LMG, de Morais DT, Domingues RF, de Aro AA, Pimentel ER, do Amaral MEC, Esquisatto MAM, Dos Santos GMT, and Mendonça FAS
- Subjects
- Animals, Blotting, Western, Burns immunology, Burns metabolism, Burns pathology, Collagen Type I metabolism, Collagen Type I radiation effects, Collagen Type III metabolism, Collagen Type III radiation effects, Dose-Response Relationship, Radiation, Fibroblasts radiation effects, Gallium, Glycosaminoglycans metabolism, Glycosaminoglycans radiation effects, Hydroxyproline metabolism, Hydroxyproline radiation effects, Indium, Inflammation, Male, Matrix Metalloproteinase 9 metabolism, Matrix Metalloproteinase 9 radiation effects, Phosphines, Rats, Rats, Wistar, Skin immunology, Skin metabolism, Skin pathology, Transforming Growth Factor beta1 immunology, Transforming Growth Factor beta1 radiation effects, Vascular Endothelial Growth Factor A metabolism, Vascular Endothelial Growth Factor A radiation effects, Burns radiotherapy, Low-Level Light Therapy methods, Skin radiation effects, Wound Healing radiation effects
- Abstract
Purpose: This study compared different energy densities of laser on second degrees burns in rats aiming to determine the most effective dosimetry in stimulation of the healing process., Methods: Burns were induced in the dorsal skin of 54 animals divided into three groups (n: 18): 1-without treatment; 2-irradiated lesions by the Indium Gallium Phosphide (InGaP) 670nm (4.93J/cm
2 ) laser; 3-irradiated lesions by the InGaP-670nm (9.86J/cm2 ) laser. Samples were collected on the 2, 10 and 18 days after injury for structural, morphometry, biochemical analysis and Western blotting., Results: The energy densities examined were effective in significantly increasing the total number of fibroblasts and blood vessels and reduce the number of inflammatory cells particularly in irradiated lesions with 9.86J/cm2 . This same energy density significantly increased the amount of GAGs (Glycosaminoglycans), decreased the TGF-β1 (Transforming Growth Factor β1) and increased the VEGF (Vascular and Endothelial Growth Factor) during the experimental period. This energy density also significantly increased the Collagen type I and decreased Collagen type III and the active isoform of metalloproteinase 9 (MMP-9)., Conclusions: The energy density of 9.86J/cm2 was more effective in promoting cellular responses related to neoangiogenesis, decreasing inflammation and collagen fibers reorganization., (Copyright © 2017 Elsevier Ltd and ISBI. All rights reserved.)- Published
- 2017
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