1. Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice.
- Author
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Golec M, Lemieszek MK, Skórska C, Sitkowska J, Zwoliński J, Mackiewicz B, Góra-Florek A, Milanowski J, and Dutkiewicz J
- Subjects
- Administration, Inhalation, Aerosols, Aging metabolism, Alveolitis, Extrinsic Allergic etiology, Animals, Antimicrobial Cationic Peptides, Cell Extracts toxicity, Disease Models, Animal, Female, Mice, Mice, Inbred C57BL, Pantoea chemistry, Pantoea immunology, Protein Precursors analysis, Alveolitis, Extrinsic Allergic metabolism, Cathelicidins analysis, Chemokine CXCL10 analysis, Chemokine CXCL9 analysis, Laminin analysis, Toll-Like Receptors analysis
- Abstract
Introduction: Hypersensitivity pneumonitis (HP) is an interstitial lung disease caused by unresolved inflammation and tissue repair pathologies triggered by repeated organic dust exposure. The aim of the study was to investigate changes in levels of the cathelicidin related antimicrobial peptide (CRAMP), laminin (LAM-A1), selected Toll-like receptors (TLR) and chemokines in experimental HP in mice., Materials and Methods: Three and 18-month-old female C57BL/6J mice underwent inhalations of the saline extract of Pantoea agglomerans cells, Gram-negative bacterium common in organic dust and known for its pathogenic impact. The inhalations were repeated daily (28 days). ELISA was used for measuring in lung tissue homogenates concentration of CRAMP, LAM-A1, TLR2, TLR4, TLR8, CXCL9 (chemokine [C-X-C motif] ligand) and CXCL10., Results: Levels of TLR2, TLR4 and CXCL9 were significantly higher in both young and old mice lungs already after 7 days of inhalations, while significant increase of LAM-A1 and CXCL10 was noted after 28 days, compared to untreated samples. TLR8 level was significantly augmented only in young mice. Only CRAMP level significantly declined. Significantly higher TLR8 and CXCL9 concentration in untreated samples were noted in old animals compared to young ones., Conclusion: Significant alterations of the examined factors levels indicate their role in HP pathogenesis., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Published
- 2015
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