1. Cryopreservation of rat hepatocytes with disaccharides for cell therapy.
- Author
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Cardoso LMDF, Pinto MA, Henriques Pons A, and Alves LA
- Subjects
- Animals, Cell Survival drug effects, Cell- and Tissue-Based Therapy, Cells, Cultured, Freezing, Glucose pharmacology, Humans, Male, Rats, Rats, Wistar, Sucrose pharmacology, Trehalose pharmacology, Cryopreservation methods, Cryoprotective Agents pharmacology, Dimethyl Sulfoxide pharmacology, Disaccharides pharmacology, Hepatocytes drug effects
- Abstract
Cryopreservation of hepatocytes is a crucial step in the implementation of cell therapy for treating certain liver diseases. In the present study we investigated the effect of the some disaccharides on the cryopreservation of rat hepatocytes. Liver cells were frozen in media in the presence or absence of low concentrations of Me
2 SO (5% Me2 SO) supplemented with varying concentrations of disaccharides (sucrose, glucose and trehalose). After 7 days of cryopreservation, the hepatocytes were thawed and viability was measure by exclusion of trypan blue and by the MTT technique, as well as by determining albumin production. Among the investigated disaccharides and concentrations, 0.2 M trehalose showed the best overall outcome. Compared to the use of Me2 SO alone, significant improvement in post-thaw cell viability was observed. The new solution may reduce Me2 SO side effects on patients and improve the viability and quality of cryopreserved hepatocytes., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2017
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