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Lake Baikal, the largest freshwater lake by volume, provides drinking water and aquatic food supplies to over 2.5 million people. However, the lake has been contaminated with recalcitrant pollutants released from surrounding industrial complexes, agriculture, and natural lands, thereby increasing the risk of their bioaccumulation in fish and seals. Yet, a collective analysis of historical concentration data and their bioaccumulation potential as well as what factors drive their accumulation in fish or seals remains largely unknown. We analyzed concentration data from 42 studies collected between 1985 and 2019 in water, sediment, fish, and seals of Lake Baikal. Heavy metals had the highest concentrations in water and biota followed closely by polycyclic aromatic hydrocarbons (PAHs) and organochlorines. Among organochlorines, polychlorinated biphenyls (PCBs) showed the highest levels in water, surpassing hexachlorocyclohexane (HCH) concentrations, particularly after normalizing to solubility. While naphthalene and phenanthrene exhibited the highest average concentrations among polycyclic aromatic hydrocarbons (PAHs), their relative concentrations significantly decreased upon solubility normalization. The analysis confirmed that bioconcentration and biomagnification of organochlorine pesticides, PCBs, PAHs, and heavy metals depend primarily on source strength to drive their concentration in water and secondarily on their chemical characteristics as evidenced by the higher concentrations of low-solubility PCBs and high molecular weight PAHs in water and sediment. The differential biomagnification patterns of Cu, Hg, and Zn compared to Pb are attributed to their distinct sources and bioavailability, with Cu, Hg, and Zn showing more pronounced biomagnification due to prolonged industrial release, in contrast to the declining Pb levels. Dibenzo-p-dioxins were detected in sediment and seals, but not in water or fish compartments. These data highlight the importance of addressing even low concentrations of organic and inorganic pollutants and the need for more consistent and frequent monitoring to ensure the future usability of this and other similar essential natural resources., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Litter pollution is a growing concern, including for Antarctica and the species that inhabit this ecosystem. In this study, we investigated the microplastic contamination in three seal species that inhabit the Western Antarctic Peninsula: crabeater (Lobodon carcinophaga), leopard (Hydrurga leptonyx) and Weddell (Leptonychotes weddellii) seals. Given the worldwide ubiquity of this type of contaminant, including the Southern Ocean, we hypothesized that the three seal species would present anthropogenic debris in their feces. We examined 29 scat samples of crabeater (n = 5), leopard (n = 13) and Weddell (n = 11) seals. The chemical composition of the items found were identified using micro-Raman and micro-FTIR spectroscopies. All the samples of the three species presented anthropic particles (frequency of occurrence - %FO - 100 %). Fibers were the predominant debris, but fragments and filaments were also present. Particles smaller than 5 mm (micro debris) were predominant in all the samples. Leopard seals ingested significantly larger micro-debris in comparison with the other seal species. The dominant color was black followed by blue and white. Micro-Raman and micro-FTIR Spectroscopies revealed the presence of different anthropogenic pigments such as reactive blue 238, Indigo 3600 and copper phthalocyanine (blue and green). Carbon black was also detected in the samples, as well as plastic polymers such as polystyrene, polyester and polyethylene terephthalate (PET), polyamide, polypropylene and polyurethane These results confirm the presence of anthropogenic contamination in Antarctic seals and highlight the need for actions to mitigate the effects and reduce the contribution of debris in the Antarctic ecosystem., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Julieta Denise Cebuhar reports financial support was provided by Society for Marine Mammalogy. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier B.V.)

Although considered one of the most pristine ecosystems, Antarctica has been largely influenced by human activities during the last 50 years, affecting its unique biodiversity. One of the major global threats to health is the emergence of antibiotic-resistant bacteria that may be actively transferred to wildlife. We cultured and tested for antibiotic resistance in 137 cloacal and fresh fecal samples of several avian and marine mammal species from the Antarctic Peninsula, the most impacted area in Antarctica. Alarmingly, 80 % of the isolates showed antibiotic resistance, either phenotypically or genotypically. Most of the resistant bacteria, such as Enterobacteriaceae and Enterococcus species, are part of local gastrointestinal microbiota. Penguins and pinnipeds harbored a great diversity of antibiotic resistance and must be eligible as sentinels for future studies. These results show that antibiotic resistance has rapidly transferred to bacteria in Antarctic wildlife, which is a global matter of concern., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Phthalates are used in plastics, found throughout the marine environment and have the potential to cause adverse health effects. In the present study, we quantified blubber concentrations of 11 phthalates in 16 samples from stranded and/or free-living marine mammals from the Norwegian coast: the killer whale (Orcinus orca), sperm whale (Physeter macrocephalus), long-finned pilot whale (Globicephala melas), white-beaked dolphin (Lagenorhynchus albirostris), harbour porpoise (Phocoena phocoena), and harbour seal (Phoca vitulina). Five compounds were detected across all samples: benzyl butyl phthalate (BBP; in 50 % of samples), bis(2-ethylhexyl) phthalate (DEHP; 33 %), diisononyl phthalate (DiNP; 33 %), diisobutyl phthalate (DiBP; 19 %), and dioctyl phthalate (DOP; 13 %). Overall, the most contaminated individual was the white-beaked dolphin, whilst the lowest concentrations were measured in the killer whale, sperm whale and long-finned pilot whale. We found no phthalates in the neonate killer whale. The present study is important for future monitoring and management of these toxic compounds., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Plastic pollution is having devastating consequences for marine organisms across the planet. However, the population level effects of macroplastic pollution remain difficult and costly to quantify. As a result, there is a need for alternative approaches to evaluate species risk to plastic pollution and inform management needs. We apply a trait-based framework for macroplastic pollution to develop a relative vulnerability index-informed by three dimensions: likelihood of exposure, species' sensitivity, and population resilience-for marine mammals, seabirds, and sea turtles found in Hawai'i. This index ranks 63 study species based on their population level vulnerability to macroplastic pollution, with the highest scoring species being the most vulnerable. Our results indicate that ducks, waders, and noddies with large populations were the least vulnerable to macroplastics, while the most vulnerable were the Hawaiian monk seal, sea turtles, baleen whales, and some albatross and petrel species. This index can inform species in need of population monitoring in Hawai'i, and direct other management priorities (e.g., locations for clean-ups or booms). More broadly, this work exemplifies the value of qualitative risk assessment approaches for better understanding the population level effects of macroplastic pollution and showcases how vulnerability indices can be used to inform management priorities., Competing Interests: Declaration of competing interest No authors have any interests to declare., (Copyright © 2023 Elsevier B.V. All rights reserved.)

The aim of this study was to describe the pathology in seals from which Listeria monocytogenes was isolated and investigate if the lesions' nature and severity were related to the phylogeny of isolates. L. monocytogenes was isolated from 13 of 50 (26%) dead grey seal (Halichoerus grypus) pups, six (12%) in systemic distribution, on the Isle of May, Scotland. Similar fatal L. monocytogenes-associated infections were found in a grey seal pup from Carnoustie, Scotland, and a juvenile harbour seal (Phoca vitulina) in the Netherlands. Whole genome sequencing of 15 of the L. monocytogenes isolates identified 13 multilocus sequence types belonging to the L. monocytogenes lineages I and II, but with scant phenotypic and genotypic antimicrobial resistance and limited variation in virulence factors. The phylogenetic diversity present suggests there are multiple sources of L. monocytogenes, even for seal pups born in the same colony and breeding season. This is the first description of L. monocytogenes isolated from, and detected in lesions in, pinnipeds and indicates that infection can be systemic and fatal. Therefore, listeriosis may be an emerging or overlooked disease in seals with infection originating from contamination of the marine environment., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Marine mammals are recognized sentinels of ecosystem health. They are susceptible to the accumulation and biomagnification of pollutants, which constitute one of the greatest threats to their survival. Legacy, such as organochlorine pesticides, and emerging contaminants, like microplastics and pharmaceuticals, may have effects on marine mammals' health at individual and population levels. Therefore, the evaluation of the risks associated with pollutants in this group is of great importance. The aim of this review is to provide information on the occurrence of legacy and emerging contaminants in marine mammals that inhabit Argentine waters. Also, to identify knowledge gaps and suggest best practices for future research. Reports of legacy contaminants referring to organochlorine pesticides and polychlorinated biphenyls were found in five species of cetaceans and two of pinnipeds. With respect to emerging pollutants, the presence of plastics was only evaluated in three species. Reported data was from at least a decade ago. Therefore, it is necessary to update existing information and conduct continuous monitoring to assess temporary trends in pollutants. All the studies were carried out in the province of Buenos Aires and Northern Patagonia indicating a knowledge gap in the southern zone of the Argentine Sea. In addition, pollutants of global environmental concern that have not been studied in Argentina are discussed. Future studies should fill these gaps and a greater effort to understand the relationships between pollutants and their effects on marine mammals is suggested. This issue will make it possible to determine thresholds for all the substances and species evaluated in order to carry out more detailed risk assessments and make decisions for the conservation of marine mammals in Argentine waters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Environmental pollution is a growing threat to wildlife health and biodiversity. The relationship between marine mammals and pollutants is, however, complex and as new chemicals are introduced to ecosystems alongside concomitant, interacting threats such as climate change and habitat degradation, the cumulative impact of these stressors to wildlife continues to expand. Understanding the health of wildlife populations requires a holistic approach to identify potential threatening processes. In the context of environmental pollution in little studied wildlife species, it is important to catalogue the current exposome to develop effective biomonitoring programs that can support diagnosis of health impacts and management and mitigation of pollution. In New South Wales, Australia, the New Zealand fur seal (Arctocephalus forsteri) is a resident species experiencing population growth following devastating historic hunting practices. This study presents a retrospective investigation into the exposure of New Zealand fur seals to a range of synthetic organic compounds and essential and non-essential trace elements. Liver tissue from 28 seals were broadly analyzed to assess concentrations of organochlorine and organophosphate pesticides, polychlorinated biphenyls, per- and polyfluoroalkyl substances, and essential and non-essential trace elements. In addition to contributing extensive pollution baseline data for the species, the work explores the influence of sex, age, and body condition on accumulation patterns. Further, based on these findings, it is recommended that a minimum of 11 juvenile male New Zealand fur seals are sampled and analyzed annually in order to maintain a holistic biomonitoring approach for this population., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Urogenital carcinoma (UGC) is prevalent among California sea lions (Zalophus californianus), while less is known concerning UGC among Steller sea lions (Eumetopias jubatus). Our objective was to investigate associations between UGC and polybrominated diphenyl ethers (PBDEs) among both sea lion species. Twenty-nine California sea lions and 20 Steller sea lions were lethally removed from the Columbia River Basin, Oregon, USA between 2020 and 2021, under Section 120 of the Marine Mammal Protection Act. UGC was diagnosed through gross necropsy and histopathology. Forty PBDE congeners were analyzed in blubber, including BDE-209, a potential carcinogen. Twenty (69 %) California sea lions and one (5 %) Steller sea lion were diagnosed with UGC. All cases were identified as early stage UGC, aside from one California sea lion with more advanced stage UGC. Among California sea lions, associations between PBDEs and UGC were analyzed using logistic regression. In the adjusted model, BDE-209 (log 2 -transformed) was associated with increased odds of UGC [Odds Ratio (OR): 4.68, 95 % confidence interval: 1.04, 21.0, OR p-value = 0.044). This is the first study to report BDE-209 concentrations in sea lion blubber. The percentages of California and Steller sea lions diagnosed with UGC were higher than expected for wild (non-stranded) sea lions. Our results suggested blubber BDE-209 was potentially associated with UGC in California sea lions in the Columbia River Basin., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Marine mammals are considered sentinel species and may act as indicators of ocean health. Plastic residues are widely distributed in the oceans and are recognised as hazardous contaminants, and once ingested can cause several adverse effects on wildlife. This study aimed to identify and characterise plastic ingestion in the Guiana dolphins (Sotalia guianensis) from the Southwestern Tropical Atlantic by evaluating the stomach contents of stranded individuals through KOH digestion and identification of subsample of particles by LDIR Chemical Imaging System. Most of the individuals were contaminated, and the most common polymers identified were PU, PET and EVA. Microplastics were more prevalent than larger plastic particles (meso- and macroplastics). Smaller particles were detected during the rainy seasons. Moreover, there was a positive correlation between the stomach content mass and the number of microplastics, suggesting contamination through trophic transfer., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Per- and polyfluoroalkyl substances (PFASs) are bioaccumulative and associated with adverse effects in both wildlife and humans. The occurrence of 33 PFASs was assessed in the plasma, liver, blubber, and brain of 18 Baikal seals (Phoca sibirica) (16 pups and 2 adult females) from Lake Baikal, Russia (in 2011). Of the 33 congeners analysed for: perfluorooctanosulfonic acid (PFOS), 7 long chain perfluoroalkyl carboxylic acids (C 8 -C 14 PFCAs) and 1 branched PFCA (perfluoro-3,7-dimethyloctanoic acid; P37DMOA) were most frequently detected. The PFASs in plasma and liver with the highest median concentrations were legacy congeners: perfluoroundecanoic acid (PFUnA; plasma: 11.2 ng/g w.w.; liver: 7.36 ng/g w.w.), PFOS (plasma: 8.67 ng/g w.w.; liver: 9.86 ng/g w.w.), perfluorodecanoic acid (PFDA; plasma: 5.13 ng/g w.w.; liver: 6.69 ng/g w.w.), perfluorononanoic acid (PFNA; plasma: 4.65 ng/g w.w.; liver: 5.83 ng/g w.w.) and perfluorotridecanoic acid (PFTriDA; plasma: 4.29 ng/g w.w.; liver: 2.55 ng/g w.w.). PFASs were detected in the brain of Baikal seals, indicating that PFASs cross through the blood-brain barrier. In blubber, the majority of PFASs were detected in low abundance and concentrations. In contrast to legacy PFASs, novel congeners (e.g., Gen X) were either detected infrequently or not found in Baikal seals. The worldwide occurrence of PFASs in pinnipeds was compared and lower median concentrations of PFOS were observed in Baikal seals relative to other pinnipeds. Conversely, similar concentrations of long chain PFCAs were found in Baikal seals compared to other pinnipeds. Furthermore, human exposure was assessed by estimating weekly intakes (EWI) of PFASs through Baikal seal consumption. Although PFASs concentrations were comparatively low relative to other pinnipeds, consumption of Baikal seal could exceed current regulatory guidelines., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Combining mercury and stable isotope data sets of consumers facilitates the quantification of whether contaminant variation in predators is due to diet, habitat use and/or environmental factors. We investigated inter-species variation in total Hg (THg) concentrations, trophic magnification slope between δ 15 N and THg, and relationships of THg with δ 13 C and δ 34 S in 15 fish and four marine mammal species (249 individuals in total) in coastal Arctic waters. Median THg concentration in muscle varied between species ranging from 0.08 ± 0.04 μg g -1 dw in capelin to 3.10 ± 0.80 μg g -1 dw in beluga whales. Both δ 15 N (r 2  = 0.26) and δ 34 S (r 2  = 0.19) best explained variation in log-THg across consumers. Higher THg concentrations occurred in higher trophic level species that consumed more pelagic-associated prey than consumers that rely on the benthic microbial-based food web. Our study illustrates the importance of using a multi-isotopic approach that includes δ 34 S when investigating trophic Hg dynamics in coastal marine systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The Antarctic is particularly sensitive to mercury (Hg) pollution and even low levels of Hg may cause significant damage in this fragile environment. The aim of this study was to investigate routes of mercury and methylmercury (MeHg) elimination by animals inhabiting the maritime Antarctic. The results showed that organisms at the highest trophic level (elephant seal) have the highest concentrations of THg and MeHg in both excrement and fur samples. Interspecies differences in mercury levels were observed in materials sourced from penguins of the genus Pysgocelis. 13 C and 15 N values confirmed differences in the diets and foraging areas, which may affect Hg concentration in the tissues we analyzed. Time variations in THg and MeHg concentrations were observed in the excrement of the penguin species, which may be due to periods of fasting and intense feeding closely related to egg laying and moulting stages., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Southeast (SE) Asia is a highly biodiverse region, yet it is also estimated to cumulatively contribute a third of the total global marine plastic pollution. This threat is known to have adverse impacts on marine megafauna, however, understanding of its impacts has recently been highlighted as a priority for research in the region. To address this knowledge gap, a structured literature review was conducted for species of cartilaginous fishes, marine mammals, marine reptiles, and seabirds present in SE Asia, collating cases on a global scale to allow for comparison, coupled with a regional expert elicitation to gather additional published and grey literature cases which would have been omitted during the structured literature review. Of the 380 marine megafauna species present in SE Asia, but also studied elsewhere, we found that 9.1 % and 4.5 % of all publications documenting plastic entanglement (n = 55) and ingestion (n = 291) were conducted in SE Asian countries. At the species level, published cases of entanglement from SE Asian countries were available for 10 % or less of species within each taxonomic group. Additionally, published ingestion cases were available primarily for marine mammals and were lacking entirely for seabirds in the region. The regional expert elicitation led to entanglement and ingestion cases from SE Asian countries being documented in 10 and 15 additional species respectively, highlighting the utility of a broader approach to data synthesis. While the scale of the plastic pollution in SE Asia is of particular concern for marine ecosystems, knowledge of its interactions and impacts on marine megafauna lags behind other areas of the world, even after the inclusion of a regional expert elicitation. Additional funding to help collate baseline data are critically needed to inform policy and solutions towards limiting the interactions of marine megafauna and plastic pollution in SE Asia., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Marine mammals are vulnerable to the bioaccumulation, biomagnification and lactational transfer of specific types of pollutants, such as industrial polychlorinated biphenyls (PCBs), due to their long-life spans, feeding at a high trophic level and unique fat stores that can serve as depots for these lipophilic contaminants. Currently, European countries are developing indicators for monitoring pollutants in the marine environment and assessing the state of biodiversity, requirements under both Regional Seas Conventions and European legislation. As sentinel species for marine ecosystem and human health, marine mammals can be employed to assess bioaccumulated contaminants otherwise below current analytical detection limits in water and lower trophic level marine biota. To aid the development of Regional Seas marine mammal contaminants indicators, as well as Member States obligations under descriptor 8 of the EU Marine Strategy Framework Directive, the current study aims to further develop appropriate methodological standards using data collected by the established UK marine mammal pollutant monitoring programme (1990 to 2017) to assess the trends and status of PCBs in harbour porpoises. Within this case study, temporal trends of PCB blubber concentration in juvenile harbour porpoises were analysed using multiple linear regression models and toxicity thresholds for the onset of physiological (reproductive and immunological) endpoints were applied to all sex-maturity groups. Mean PCB blubber concentrations were observed to decline in all harbour porpoise Assessment Units and OSPAR Assessment Areas in UK waters. However, a high proportion of animals were exposed to concentrations deemed to be a toxicological threat, though the relative proportion declined in most Assessment Units/Areas over the last 10 years of the assessment. Recommendations were made for improving the quality of the assessment going forward, including detailing monitoring requirements for the successful implementation of such an indicator., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Ringed seals (Pusa hispida) play a crucial role in Arctic food webs as important pelagic predators and represent an essential component of Inuvialuit culture and food security. Plastic pollution is recognized as a global threat of concern, and Arctic regions may act as sinks for anthropogenic debris. To date, mixed evidence exists concerning the propensity for Canadian Arctic marine mammals to ingest and retain plastic. Our study builds on existing literature by offering the first assessment of plastic ingestion in ringed seals harvested in the western Canadian Arctic. We detected no evidence of microplastic (particles ≥80 μm) retention in the stomachs of ten ringed seals from the Inuvialuit Settlement Region (ISR) in the Northwest Territories, Canada. These results are consistent with previous studies that have found that some marine mammals do not accumulate microplastics in evaluated regions., Competing Interests: Declaration of competing interest The authors have no competing interests that could have influenced the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Microplastics (MPs, < 5 mm in size) are highly bioavailable to many taxa within the marine ecosystem, either ingested directly or indirectly through trophic transfer from polluted prey. The ingestion analysis of these MPs from top predators, such as pinnipeds in Mexico, is relatively unexplored. Forty-eight scats from California sea lions were collected on six rookeries along the Gulf of California. From these scat samples, 294 suspected MPs particles were classified and chemically analyzed; 34% were synthetic and semi-synthetic, and 66% were non-synthetic. Blue-colored polyethylene terephthalate fibers were the most common type of MP registered. During laboratory work, multiple contamination control measures were implemented. Although the ingestion pathway is still unknown, our results support the other authors that suggest the potential trophic transfer of MPs to top predators and incidental ingestion while foraging. The particles documented here provide important baseline information for future MP research in the Gulf of California., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Plastic debris is of particular concern due to its abundance, and its persistence in the environment. In Argentina, the impact of plastic debris has been documented with a significant increase during the last years. However, evidence of debris ingestion remains low compared to other regions of the world. Between 2020 and 2022, one of the twenty-three individuals analyzed in this study (seven species: five birds and two marine mammals) had ingested plastic debris. A single macroplastic piece was found in the Great Grebe (Podiceps major). It was a hardy yellow elastic band that appears to be a packaging band in agreement with the debris category with the greatest number of reports. This study is a contribution to the few records of plastic ingestion in birds, on the east coast of South America., Competing Interests: Declaration of competing interest The authors declare not to have any interest conflicts., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Pinnipeds are sentinel species for marine pollution, but their role as vectors of trace elements (TEs) or rare earth elements (REEs) to ecosystems has been poorly studied. The present study tested pinniped feces for 61 elements, including REEs. Feces of adult seals (Mirounga leonina, Hydrurga leptonyx) from Fildes Bay, King George Island, Antarctica, were analyzed by ICP-MS. TEs varied by several orders of magnitude across the suite examined herein, with Fe, Al, Zn, Mn, HgII and Sr as the top six in both species. Of the REEs, Ce, Dy, Er, Eu, Gd, Ho, La, Lu, Nd, Pr, Sc, Sm, Tb, Y and Yb were found consistently in all samples and ranged from 0.935 to 0.006 μg g -1 d.w. The results show that both species act as biovector organisms of TEs and REEs through feces in remote environments, whose actual impacts and long-term fate need further exploration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Assessing the structure and composition of gut microbiota of sentinel species such as spotted seals (Phoca largha) is a potential tool for assessing the health of the marine mammals and their habitats. However, the link between the host microbiome and their habitat is poorly understood. In this study, microbial communities in the habitat (sea ice and water) and marine mammalian host (fecal matter from P. largha) were evaluated in samples obtained from the Liaodong Bay, China during population aggregation period. Results from high-throughput sequencing showed that the bacterial communities in P. largha fecal matter were less rich and diverse compared to those from the water and ice samples. Significant differences in the composition and function of bacterial communities were also found among the water, ice, and fecal samples, in which sample type and sampling site had the greatest impact on composition and function variations, respectively. Several potential pathogenic bacteria and bacteria with functions associated with human disease were significantly enhanced in the communities of P. largha feces compared to those of surrounding environments. The ratios of environmental microorganisms sourced from the P. largha fecal matter were estimated. The results showed that certain bacteria in P. largha-inhabited fecal matter were associated with sea ice and had specific antibiotic resistance and infectious capacity. These findings provide critical data for monitoring the health of marine mammals and their habitats, which is essential for predicting the impact of anthropogenic disturbances on marine ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier B.V.)

There has been a considerable number of reports on Hg concentrations in Arctic mammals since the last Arctic Monitoring and Assessment Programme (AMAP) effort to review biological effects of the exposure to mercury (Hg) in Arctic biota in 2010 and 2018. Here, we provide an update on the state of the knowledge of health risk associated with Hg concentrations in Arctic marine and terrestrial mammal species. Using available population-specific data post-2000, our ultimate goal is to provide an updated evidence-based estimate of the risk for adverse health effects from Hg exposure in Arctic mammal species at the individual and population level. Tissue residues of Hg in 13 species across the Arctic were classified into five risk categories (from No risk to Severe risk) based on critical tissue concentrations derived from experimental studies on harp seals and mink. Exposure to Hg lead to low or no risk for health effects in most populations of marine and terrestrial mammals, however, subpopulations of polar bears, pilot whales, narwhals, beluga and hooded seals are highly exposed in geographic hotspots raising concern for Hg-induced toxicological effects. About 6% of a total of 3500 individuals, across different marine mammal species, age groups and regions, are at high or severe risk of health effects from Hg exposure. The corresponding figure for the 12 terrestrial species, regions and age groups was as low as 0.3% of a total of 731 individuals analyzed for their Hg loads. Temporal analyses indicated that the proportion of polar bears at low or moderate risk has increased in East/West Greenland and Western Hudson Bay, respectively. However, there remain numerous knowledge gaps to improve risk assessments of Hg exposure in Arctic mammalian species, including the establishment of improved concentration thresholds and upscaling to the assessment of population-level effects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Mercury (Hg) is a well-known toxicant in wildlife and humans. High total Hg concentrations ([THg]) have been reported in central California harbor seals Phoca vitulina richardii. We evaluated the effects of presence/absence of early natal coat (lanugo), year (2012 to 2017), sex, stranding location, and trophic ecology (ẟ 13 C and ẟ 15 N values) on hair [THg] along coastal central California. Also examined were [THg] effects on growth rates of pups in rehabilitation and probability of release (e.g., successful rehabilitation). The [THg] ranged from 0.46-81.98 mg kg -1 dw, and ẟ 15 N and ẟ 13 C ranged from 13.6-21.5‰, and -17.2 to -13.0‰, respectively. Stranding location, year, and presence of lanugo coat were important factors explaining variation in [THg]. Seals from Sonoma and San Mateo County had higher [THg] than other locations. Seals with full or partial lanugo coat had lower [THg]. Seals from 2016 and 2017 had higher [THg] than those from 2015. Hair [THg] exceeded lower and upper toxicological thresholds (>20 mg kg -1 by year (5.88% to 23.53%); >30 mg kg -1 (0% to 12.31%)) with a pronounced increase from 2015 to 2016. Pups in 2017 had significantly higher odds ratio of [THg] above 20 mg kg -1 than pups of 2015, and pups in 2016 had significantly higher odds ratio than those from 2013 and 2015 (similar when using 30 mg kg -1 ). Pups in Sonoma County had the highest odds ratio for [THg] in lanugo above 20 mg kg -1 . ẟ 15 N values were higher in 2015-2017, particularly relative to 2014, probably associated with the El Niño event. The [THg] was not a good predictor for probability of release and mass-specific growth rates in captivity. Further investigation of temporal trends of [THg] in harbor seals is warranted given the relatively high percentage of samples exceeding threshold values, particularly in the most recent sampling years., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Marine pollution is increasing, and pinnipeds are commonly affected by entanglement in waste. We investigated entanglement rates, common materials, and the demographic profile of Cape fur seals (Arctocephalus pusillus pusillus) affected at two Namibian colonies. Overall, we identified 366 cases of entanglement, and present a global rate of entanglement of 0.17%. Entanglement rates were 0.17% and 0.15% for the Pelican Point and Cape Cross colonies, respectively. We identified 17% more entanglements through photographs than binocular scans. Of the 347 entanglements analysed in detail, juveniles were most commonly affected and fishing materials were the primary cause of entanglements (53%), with 8% of entangled seals exhibiting 'very severe' injuries. Overall, 191 individuals were successfully disentangled, and citizen scientists contributed 51% of total entanglement data. We highlight the negative impact of plastic marine waste among seals and the importance of disentanglement for individual animal welfare., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Per and polyfluorinated substances (PFAS) exposure was investigated in Australian pinnipeds. Concentrations of 16 PFAS were measured in the livers of Australian sea lion (Neophoca cinerea), Australian fur seal (Arctocephalus pusillus doriferus) and a long-nosed Fur Seal (Arctocephalus forsteri) pup sampled between 2017 and 2020 from colonies in South Australia and Victoria. Findings reported in this study are the first documented PFAS concentrations in Australian pinnipeds. Median and observed range of values in ng/g wet weight were highest for perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA) in the liver of N. cinerea (PFOS = 7.14, 1.00-16.9; PFOA = 2.73, 0.32-11.2; PFNA = 2.96, 0.61-8.22; n = 28), A. forsteri (PFOS = 15.98, PFOA = 2.02, PFNA = 7.86; n = 1) and A. p. doriferus (PFOS = 27.4, 10.5-2119; PFOA = 0.98, 0.32-52.2; PFNA = 2.50, 0.91-44.2; n = 20). PFAS concentrations in A. p. doriferus pups were significantly greater (p < 0.05) than in N. cinerea pups for all PFAS except PFOA and were of similar magnitude to those reported in northern hemisphere marine animals. These results demonstrate exposure differences in both magnitude and PFAS profiles for N. cinerea in South Australia and A. p. doriferus in Victoria. This study reports detectable PFAS concentrations in Australian pinniped pups indicating the importance of maternal transfer of these toxicants. As N. cinerea are endangered and recent declines in pup production has been reported for A. p. doriferus at the colony sampled, investigation of potential health impacts of these toxicants on Australian pinnipeds is recommended., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

The study of ecological niche segregation in sympatric species is essential to understand ecosystem functioning and its response to potential changes. In the North Sea, sympatric grey and harbour seals may present competition for food resources sustained by intense fishing activities and recent increase of seal populations. In order to coexist and reduce inter-specific competition, sympatric species must segregate at least one aspect of their ecological niches: temporal, spatial or resource segregation. We aim to study the foraging resources and foraging distributions of grey seals and harbour seals and the potential competition between these species in the North Sea. Therefore, we analysed stable isotopic composition of C, N and S (δ 13 C, δ 15 N and δ 34 S values), and the concentrations of Hg and Se in blood of harbour and grey seals from the North Sea. Blood samples were collected on 45 grey seals and 37 harbour seals sampled along German and Scottish coasts. Stable isotope ratios were performed with an isotope ratio mass spectrometer coupled to an N-C-S elemental analyser for automated analyses. Total mercury concentrations (T-Hg) were determined by atomic absorption spectroscopy and Se concentrations by ICP-MS. The multi-tracer approach shown spatial and resource partitioning within grey and harbour seal living along German and Scottish coasts. Data indicate 1) the offshore foraging distribution of grey seals as reflected by the lower δ 15 N values and T-Hg concentrations and higher Se concentrations and 2) the inshore foraging distribution of harbour seals because of higher δ 15 N values and T-Hg concentrations and lower Se concentrations. The SIAR mixing model revealed 3) a more selective diet of grey seals compared to harbour seals and 4) the importance of sandeels in grey seal diet reflected by their high δ 34 S values. Lastly, diet ellipse overlaps between grey seals and harbour seals sampled along the German coasts suggested 5) a potential sharing of food resources, possibly due to the increase number of grey seals number in this area during the foraging season - all year except breeding and moulting periods. The multi-tracer approach of this study provides a more robust discrimination among diet resources and spatial foraging distributions of grey seals and harbour seals in the North Sea., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Marine mammals and the ecological functions they provide to coastal and pelagic ecosystems are increasingly threatened by the intensification of anthropogenic impacts. The Uruguayan coastline throughout the 20th century, like other coastal environments worldwide, has been the sink of a variety of trace metals derived from the rapid urbanization and industrialization of related land areas. This coastline is inhabited by two species of pinnipeds trophically and spatially segregated. Otaria byronia feeds in coastal environments while Arctocephalus australis preys mainly offshore. The present study aimed to analyze historic changes in concentrations of trace elements in teeth of both species from 1941 to the present day. We analyzed the dentin of 94 canine teeth using stable isotope analysis (δ 13 C) and ICP-MS to determine their feeding areas and the concentration of 10 trace elements (Al, As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) respectively. The concentration of Cr was significantly higher during '70-'80s, in both species coinciding with tannery industry development. Both species of pinnipeds have been differentially exposed to trace elements depending on their feeding area. A pelagic diet, possibly based on squid, increased the concentration of Cd in A. australis, while O. byronia has been more exposed to anthropogenic Pb and Cu associated to a costal and more benthic diet. Our results highlight dentin as a reliable matrix for historic studies on the exposure to trace elements. In light of our results, the O. byronia's declining population could be the result of the synergistic effects of trace elements together with other ecological pressures faced in their environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Toxoplasma gondii infections are common in humans and animals worldwide. T. gondii causes mortality in several species of marine mammals, including threatened Southern sea otters (Enhydra lutris) and endangered Hawaiian monk seals (Monachus schauinslandi). Marine mammals are now considered sentinels for environmental exposure to protozoan agents contaminating marine waters, including T. gondii oocysts. Marine mammals also serve as food for humans and can result in foodborne T. gondii infections in humans. The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, and genetic diversity of T. gondii infecting marine mammals in the past decade. The role of genetic types of T. gondii and clinical disease is discussed., (Published by Elsevier B.V.)

Marine fauna in the California Current System is susceptible to entanglement in anthropogenic debris. We examined beach survey data from six California counties to describe trends of entangled marine birds and mammals (1997-2017). Surveyors reported 357 cases of entanglements among 65,604 carcasses. Monterey County had the greatest average entanglement rate (0.007) of surveyed counties, however, was not statistically different from Santa Cruz (p > 0.05). Twenty-six seabird species (97%) and three marine mammal species (3%), and three non-marine birds were affected. Numerically, Common Murre (23%), Brandt's Cormorant (13%), Western Gull (9.6%), Sooty Shearwater (8%) and Brown Pelican (7%) were the most affected due to abundance, but their entanglement rates were not statistically different (p > 0.05). The most vulnerable species were those frequently documented as entanglement despite low deposition numbers (Merganser spp. 25%). Entangling material consisted primarily of monofilament line (some hooks/lures), but other entanglement items were reported., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Plastic pollution is an omnipresent problem that threatens marine animals through ingestion and entanglement. Marine mammals are no exception to this rule but their interaction with plastic remains understudied in the Mediterranean Sea. Here we highlight this problem by analyzing the stomach contents of 34 individuals from seven odontocete species stranded in Greece. Macroplastic (>5 mm) was found in the stomachs of nine individuals from four species (harbour porpoise Phocoena phocoena, Risso's dolphin Grampus griseus, Cuvier's beaked whale Ziphius cavirostris and sperm whale Physeter macrocephalus) with the highest frequency of occurrence in sperm whales (60%). Gastric blockage from plastic was presumably lethal in three cases, with plastic bags being the most common finding (46%). Plastic ingestion is of particular conservation concern for the endangered Mediterranean sperm whales. A regular examination of stranded cetaceans with a standardised protocol is critical for allowing spatiotemporal comparisons within and across species., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Oceanic plastic pollution is a growing worldwide environmental concern, endangering numerous marine species. Pinnipeds are particularly susceptible to entanglement, especially in abandoned, lost or discarded fishing gear and packaging straps. We searched three international databases to compile a comprehensive review of all reported pinniped entanglements over the last 40 years, with the aim to identify areas of concern and foci for mitigation. The majority of published records of entanglement emanate from North America and Oceania and are focused on a few populous species (notably, Zalophus californianus and Arctocephalus gazella). Reporting bias, skewed research effort and incomplete understanding of plastic pollution and pinniped abundance overlap, combine to cloud our understanding of the entanglement problem. Broader geographical effort in entanglement data collection, reporting of such data, and improved quantification of the proportions of populations, sexes and ages that are most susceptible, will aid our efforts to pinpoint priority mitigation measures., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Active acoustic sensors are widely used in oceanographic and environmental studies. Although many have nominal operating frequencies above the range of marine mammal hearing, they can produce out-of-band sound that may be audible to marine mammals. Acoustic emissions from four active acoustic transducers were characterized and compared to marine mammal hearing thresholds. All four transducers had nominal operating frequencies above the reported upper limit of marine mammal hearing, but produced measurable sound below 160 kHz. A spatial map of the acoustic emissions of each sonar is used to evaluate potential effects on marine mammal hearing when the transducer is continuously operated from a stationary platform. Based on the cumulative sound exposure level metric, the acoustic emissions from the transducers are unlikely to cause temporary threshold shifts in marine mammals, but could affect animal behavior. The extent of audibility is estimated to be, at most, on the order of 100 m., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Oil spills of unknown origin were detected in three oil-fouled, ice-associated seals from the Alaska Bering Strait region collected by Alaska Native subsistence hunters during fall 2012. Bile analyses of two oiled seals indicated exposure to fluorescent polycyclic aromatic hydrocarbon (PAH) metabolites but levels of some metabolites were similar to or lower than biliary levels in harvested unoiled ice seals. Oiled seals had elevated tissue PAH concentrations compared to tissue levels of PAHs determined in unoiled ice seals. However, regardless of oiling status, tissue PAH levels were relatively low (<50 ng/g, wet weight) likely due to rapid PAH metabolism and elimination demonstrated previously by vertebrates. Hepatic, pulmonary, and cardiac lesions were observed in oiled seals in conjunction with measurable PAHs in their tissue and bile. This is the first study to report tissue and bile PAH concentrations and pathologic findings of oiled ice seals from the U.S. Arctic., (Published by Elsevier Ltd.)

The world's oceans today have become a place for the disposal of toxic waste, which leads to the degradation of marine mammal habitats and populations. Marine mammal cell cultures have proven to be a multifunctional tool for studying the peculiarities of the cell physiology and biochemistry of these animals as well as the destructive effects of anthropogenic and natural toxicants. This review describes the sources of marine mammal live tissues and the methods required for establishing cell cultures, their use, and long-term storage. Approaches to conserving rare animal species by applying cell biology methodologies are also discussed., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

This review focuses on the effect that ghost gear entanglement has on marine megafauna, namely mammals, reptiles and elasmobranchs. A total of 76 publications and other sources of grey literature were assessed, and these highlighted that over 5400 individuals from 40 different species were recorded as entangled in, or associated with, ghost gear. Interestingly, there appeared to be a deficit of research in the Indian, Southern, and Arctic Oceans; and so, we recommend that future studies focus efforts on these areas. Furthermore, studies assessing the effects of ghost gear on elasmobranchs, manatees, and dugongs should also be prioritised, as these groups were underrepresented in the current literature. The development of regional databases, capable of recording entanglement incidences following a minimum global set of criteria, would be a logical next step in order to analyse the effect that ghost gear has on megafauna populations worldwide., (Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.)

Entanglement in and ingestion of synthetic marine debris is increasingly recognized worldwide as an important stressor for marine wildlife, including marine mammals. Studying its impact on wildlife populations is complicated by the inherently cryptic nature of the problem. The coastal waters of British Columbia (BC), Canada provide important habitat for marine mammal species, many of which have unfavorable conservation status in the US and Canada. As a priority-setting exercise, we used data from systematic line-transect surveys and spatial modeling methods to map at-sea distribution of debris and 11 marine mammal species in BC waters, and to identify areas of overlap. We estimated abundance of 36,000 (CIs: 23,000-56,600) pieces of marine debris in the region. Areas of overlap were often far removed from urban centers, suggesting that the extent of marine mammal-debris interactions would be underestimated from opportunistic sightings and stranding records, and that high-overlap areas should be prioritized by stranding response networks., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Giardia and Cryptosporidium are protozoan parasites known to cause enteric disease in terrestrial wildlife species (mammals, reptiles and birds). Few surveys for Giardia and Cryptosporidium in marine wildlife species, such as pinnipeds, have been reported. The objective of this study was to determine the prevalence and genotype of Giardia and Cryptosporidium in two species of pinnipeds, harp seal (Phoca groenlandica) and hooded seal (Cystophora cristata), from the Gulf of St. Lawrence, Canada. Faecal samples were collected from pup and adult seals and examined for the presence of cysts of Giardia and oocysts of Cryptosporidium using microscopy and immunofluorescent staining. Tissues from the small intestine of adult seals were also collected and examined for infections using the polymerase chain reaction (PCR) technique. Giardia cysts were found in the faeces of 42% (16/38) of adult harp seals, but in none of the harp seal pups (0/20). Although Giardia cysts were not detected in faeces of adult hooded seals (0/10) using microscopy, 80% tested positive for Giardia using PCR of intestinal tissue indicative of a true replicating infection. Both harp and hooded seals harboured infections with the zoonotic strain, Giardia duodenalis Assemblage A, as determined using a nested-PCR technique to amplify a small subunit ribosomal (SSU-rRNA) gene of Giardia. Cryptosporidium was not detected by microscopy, nor using the PCR technique on intestinal tissues from any of the 68 seals examined., (Copyright 2010 Elsevier B.V. All rights reserved.)

Specific activities of the anthropogenic radionuclide, 137Cs, were determined in marine mammals from Svalbard and the Barents and North Greenland Seas. Muscle samples were collected from 12 polar bears, 15 ringed seals, 10 hooded seals, 7 bearded seals, 14 harp seals, one walrus, one white whale and one blue whale in the period 2000-2003. The mean concentrations (+/-SD) of 137Cs were: 0.72+/-0.62 Bq/kg wet weight (w.w.) for polar bears; 0.49+/-0.07 Bq/kg w.w. for ringed seals; 0.25+/-0.10 Bq/kg w.w. for hooded seals; 0.22+/-0.11 Bq/kg w.w. for bearded seals; 0.36+/-0.13 Bq/kg w.w. for harp seals; 0.67 Bq/kg w.w. for the white whale sample; 0.24 Bq/kg w.w. for the blue whale; and below detection limit for the walrus. Significant differences in 137Cs specific activities between some of the species were found. Ringed seals had higher specific activities than the other seal species in the study. Bearded seals and hooded seals had similar values, which were both significantly lower than the harp seal values. The results in the present study are consistent with previous reported results, indicating low specific activities of 137Cs in Arctic marine mammals in the Barents Sea and Greenland Sea region during the last 20 years. The species specific differences found may be explained by varying diet or movement and distribution patterns between species. No age related patterns were found in specific activities for the two species (polar bears and hooded seals) for which sufficient data was available. Concentration factors (CF) of 137Cs from seawater were determined for polar bears, ringed, bearded, harp and hooded seals. Mean CF values ranged from 79+/-32 (SD) for bearded seals sampled in 2002 to 244+/-36 (SD) for ringed seals sampled in 2003 these CF values are higher than those reported for fish and benthic organisms in the literature, suggesting bioaccumulation of 137Cs in the marine ecosystem.

Various organs--lung, trachea, liver, kidney, heart, adrenal gland, skin, spleen, thymus, lymph node, gut, thyroid, spinal cord and brain--were removed from 43 seals at dissections performed on the German North sea coast. The specimens were fixed in formalin and routinely processed for light microscopy. The major pathological findings were Lung: acute congestion with interstitial and intra-alveolar oedema; intra-alveolar haemorrhage; suppurative bronchitis and bronchopneumonia; larvae and adult forms of Parafilaroides gymnurus. Liver: acute congestion; granulomatous lesions and infiltrates of eosinophils; intravascular nematodes. Spleen: varying degrees of atrophy of the white pulp; haemosiderosis; acute congestion of the red pulp. Lymph nodes: varying degrees of atrophy of the lymphatic tissue; long-standing sinus histiocytosis with partial fibrotic obliteration of the lumina; parasitic infiltration, sometimes with the Splendore-Hoeppli phenomenon; germinal centre hyperplasia. Thymus: pronounced atrophy of the lymphatic tissue, particularly in the cortical areas. Thyroid: marked reduction in colloid content. The other organs studied were normal or showed only minor histopathological changes. The morphological findings do not allow definite conclusions to be made about the aetiology and pathogenesis of the illness and death of the seals. However, evidence has been published that the seals' illness is probably due to canine distemper virus. The atrophy of the lymphoreticular tissues is consistent with infection by this virus.

Two young women developed septic arthritis in an interphalangeal joint following a seal bite. One patient was cured with tetracycline: the other required joint arthrodesis. This entity known as "seal finger" is common among sealers. Although the infection may be cured with tetracycline, in late treated or untreated cases joint destruction may occur. No causative organism has been isolated.

A survey was conducted during 1971-1973 on the vitamin E status of Alaskan Eskomos. The subjects were 315 residents of the northern coastal villages of Wainwright and Point Hope and the southwestern inland villages of Kasigluk and Nunapitchuk. Plasma vitamin E levels for the 6- to 17-year-old subjects at Wainwright, Point Hope, and Nunapitchuk were 0.81 plus or minus 0.26, 0.90 plus or minus 0.20, and 0.84 plus or minus 0.25 mg/100 ml (mean and standard deviation), respectively. The values for adults at Wainwright, Point Hope, and Kasigluk were 1.23 plus or minus 0.27, 1.23 plus or minus 0.27, and 1.27 plus or minus 0.33 mg/100 ml, respectively. No value less than 0.30 mg/100 ml was observed. Alpha-tocopherol was the only isomer present in significant amounts. Plasma vitamin E levels did not change significantly between 6 and 17 years of age; however, a steady increase with age was observed in the 18- to 69-year-old groups. Plasma alpha-tocopherol concentrations were significantly lower in children than in adults but there were no differences attributable to sex or geographic location. Vitamin E concentration in the blood plasma was linearly correlated with cholesterol concentration. Values are reported for the vitamin E content of some native foods. This study indicates that plasma vitamin E levels in Alaskan Eskimos consuming a high meat or fish diet are comparable to those in adults of the United States consuming a mixed diet.