Search

Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species., (Copyright © 2020 The Authors. Published by Elsevier GmbH.. All rights reserved.)

Infections by the ixodid tick-transmitted bacterial pathogen Anaplasma phagocytophilum are common in domestic ruminants and cervids in the coastal areas of southern Norway. Previous experimental work has shown that A. phagocytophilum strains recovered from red deer (Cervus elaphus) are infective in lambs, but epidemiological links between infections in red deer and sheep have yet to be established. To address this shortfall, the present study explores the genotypic relatedness between A. phagocytophilum strains infecting sympatric red deer and sheep. Blood from 32 lambs grazing on tick-infested pasture, and blood and tissues from 8 red deer shot in proximity to these pastures were collected during the summer and autumn of 2007. The presence of A. phagocytophilum in these samples was determined by PCR-based methods, and genotyping of detected strains was performed using comparative sequence analysis of 16S rDNA and msp4 fragments. A. phagocytophilum DNA was detected in 12 lambs and 7 red deer, 11 and 4 individuals of which 16S rDNA and msp4 sequence data were obtained from, respectively. A total of 9 genotypes were delineated, and only different individuals of the same host species were infected with indistinguishable A. phagocytophilum genotypes. Although 3 of the red deer-infecting genotypes belonged to a cluster of exclusively deer-associated strains phylogenetically remote from those commonly encountered in sheep, one red deer-infecting genotype, although unique, clustered tightly with genotypes associated with a wide range of hosts including sheep., (Copyright © 2013 Elsevier GmbH. All rights reserved.)

Two patients presented with hard black eschars covering granulating ulcers. The ulcers healed within 6 weeks to leave small scars. The diagnosis of cowpox was confirmed by serology in both cases and in addition by polymerase chain reaction in the first. The source of infection was likely to be a rodent in the first case whilst the second was more typical in being transmitted by a cat., (Copyright 2000 The British Association of Plastic Surgeons.)