31 results on '"Bosch D"'
Search Results
2. SWAT: Agricultural water and nonpoint source pollution management at a watershed scale—Part II
- Author
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Volk, Martin, Bosch, D., Nangia, V., Narasimhan, B., Volk, Martin, Bosch, D., Nangia, V., and Narasimhan, B.
- Published
- 2016
3. SWAT: Agricultural water and nonpoint source pollution management at a watershed scale. Editorial
- Author
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Volk, Martin, Bosch, D., Nangia, V., Narasimhan, B., Volk, Martin, Bosch, D., Nangia, V., and Narasimhan, B.
- Abstract
no abstract
- Published
- 2016
4. Anaesthesia after neoadjuvant chemotherapy, immunotherapy or radiotherapy.
- Author
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Groenewold MD, Olthof CG, and Bosch DJ
- Abstract
Competing Interests: The authors declare that they have no conflicts of interest.
- Published
- 2022
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5. The santalene synthase from Cinnamomum camphora: Reconstruction of a sesquiterpene synthase from a monoterpene synthase.
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Di Girolamo A, Durairaj J, van Houwelingen A, Verstappen F, Bosch D, Cankar K, Bouwmeester H, de Ridder D, van Dijk ADJ, and Beekwilder J
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- Alkyl and Aryl Transferases genetics, Alkyl and Aryl Transferases metabolism, Cinnamomum camphora genetics, Escherichia coli genetics, Escherichia coli metabolism, Monoterpenes metabolism, Plant Proteins genetics, Plant Proteins metabolism, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Sesquiterpenes metabolism, Alkyl and Aryl Transferases chemistry, Cinnamomum camphora enzymology, Monoterpenes chemistry, Plant Proteins chemistry, Sesquiterpenes chemistry
- Abstract
Plant terpene synthases (TPSs) can mediate formation of a large variety of terpenes, and their diversification contributes to the specific chemical profiles of different plant species and chemotypes. Plant genomes often encode a number of related terpene synthases, which can produce very different terpenes. The relationship between TPS sequence and resulting terpene product is not completely understood. In this work we describe two TPSs from the Camphor tree Cinnamomum camphora (L.) Presl. One of these, CiCaMS, acts as a monoterpene synthase (monoTPS), and mediates the production of myrcene, while the other, CiCaSSy, acts as a sesquiterpene synthase (sesquiTPS), and catalyses the production of α-santalene, β-santalene and trans-α-bergamotene. Interestingly, these enzymes share 97% DNA sequence identity and differ only in 22 amino acid residues out of 553. To understand which residues are essential for the catalysis of monoterpenes resp. sesquiterpenes, a number of hybrid synthases were prepared, and supplemented by a set of single-residue variants. These were tested for their ability to produce monoterpenes and sesquiterpenes by in vivo production of sesquiterpenes in E. coli, and by in vitro enzyme assays. This analysis pinpointed three residues in the sequence which could mediate the change in product specificity from a monoterpene synthase to a sesquiterpene synthase. Another set of three residues defined the sesquiterpene product profile, including the ratios between sesquiterpene products., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2020
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6. Does Donor Status, Race, and Biological Sex Predict Organ Donor Registration Barriers?
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Quick BL, LaVoie NR, Reynolds-Tylus T, Bosch D, and Morgan SE
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- Black or African American psychology, Chicago, Female, Hispanic or Latino psychology, Humans, Male, White People psychology, Ethnicity psychology, Health Knowledge, Attitudes, Practice, Registries, Tissue Donors psychology, Tissue and Organ Procurement methods
- Abstract
Purpose: The purpose of the current study was to examine differences among bodily integrity, disgust, medical mistrust, and superstition among African Americans, Caucasians, and Latinos; females and males; and registered organ donors and non-registered potential donors., Methods: A random digit dialing phone survey was utilized to garner information pertaining to organ donation beliefs among African American (n = 200), Caucasian (n = 200), and Latino (n = 200) Chicago residents. More specifically, participants responded to measures of bodily integrity, disgust, medical mistrust, and superstition, organ donor registration status, among others., Results: The results indicated that African American and Latino participants were less likely to be registered organ donors than Caucasians (p < .001). In general, females maintained fewer barriers than males with respect to bodily integrity (p < .05), disgust (p = .01), and superstition (p = .01). With respect to organ donation barriers, bodily integrity (p < .0001) emerged as a central concern among those surveyed., Conclusion: This study highlights the significance of audience segmentation when promoting posthumous organ and tissue donation. Specifically, the results stress the importance of constructing distinct messages to non-registered potential donors compared to messages delivered to registered donors. Moreover, different barriers surfaced among females and males as well as among African American, Caucasian, and Latino residents. It is clear that a one size fits all approach will likely not work when promoting organ and tissue donation., (Copyright © 2016. Published by Elsevier Inc.)
- Published
- 2016
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7. Clinical medication review in Australia: A systematic review.
- Author
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Jokanovic N, Tan EC, van den Bosch D, Kirkpatrick CM, Dooley MJ, and Bell JS
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- Australia, Humans, Drug Utilization Review
- Abstract
Background: Clinical medication review (CMR) is a structured and collaborative service aimed at identifying and resolving medication-related problems (MRPs). This is the first systematic review of CMR research in Australia., Objective: To systematically review the processes and outcomes of CMR in community-settings in Australia., Methods: MEDLINE, EMBASE, International Pharmaceutical Abstracts (IPA), Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Library and the grey literature were searched from 2000 to February 2015. All study designs were considered. Data extraction and quality assessment were performed independently by two investigators., Results: Nine controlled studies, 34 observational and uncontrolled studies, 11 qualitative studies (focus groups and interviews) and nine survey studies were included. The CMRs resulted in identification of MRPs (n = 15 studies, mean 3.6 MPRs per CMR) and improved adherence (n = 3). Reductions in numbers of medications prescribed (n = 3 studies), hospitalizations (n = 3), potentially inappropriate prescribing (n = 3) and costs (n = 6) were demonstrated. Comparisons to a control group, predominately non-recipients of CMR, were made in eleven of 43 studies. Evidence supports additional models that promote interprofessional collaboration and timely referral following hospital discharge. Qualitative research identified low awareness of CMR among eligible non-recipients, while benefits were perceived to outweigh barriers to implementation. Underserved populations include indigenous and culturally and linguistically diverse people, recipients of palliative care, those recently discharged from hospital, people with poor medication adherence, those in rural and remote areas, older males, and younger people with long-term, persistent or serious health problems., Conclusion: The available evidence suggests CMR is beneficial in improving the quality use of medications and health outcomes. However, lack of comparator groups in many observational studies limited the strength of conclusions in relation to the impact on clinical outcomes. Addressing access gaps for underserved populations, implementing additional referral pathways, and facilitating greater collaboration between the health professionals represent opportunities for further improvement., (Copyright © 2016 Elsevier Inc. All rights reserved.)
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- 2016
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8. Inhibition of PDZ domain-mediated interactions.
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Grillo-Bosch D, Choquet D, and Sainlos M
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- Animals, Humans, Ligands, Peptides pharmacology, Protein Interaction Mapping, Drug Discovery, PDZ Domains
- Abstract
Modulating protein-protein interactions constitutes a promising strategy both for the investigation of biological mechanisms and for developing new therapeutic approaches. Among the many types of inter-actions, PDZ domain-mediated interactions (PDMIs) have emerged over the last decade as attractive targets in the drug discovery field. Indeed, these small domains are involved in the regulation of many signaling pathways and possess structural properties which are favorable for the design of competing ligands. Herein, we describe the recent approaches developed to inhibit this class of protein-protein interactions.
- Published
- 2013
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9. N-glycan occupancy of Arabidopsis N-glycoproteins.
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Song W, Mentink RA, Henquet MG, Cordewener JH, van Dijk AD, Bosch D, America AH, and van der Krol AR
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- Amino Acid Sequence, Arabidopsis genetics, Arabidopsis Proteins chemistry, Glycopeptides isolation & purification, Glycosylation, Membrane Glycoproteins chemistry, Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase metabolism, Arabidopsis chemistry, Glycoproteins chemistry
- Abstract
Most secreted proteins in eukaryotes are modified on the amino acid consensus sequence NxS/T by an N-glycan through the process of N-glycosylation. The N-glycans on glycoproteins are processed in the endoplasmic reticulum (ER) to different mannose-type N-glycans or, when the protein passes through the Golgi apparatus, to different complex glycan forms. Here we describe the capturing of N-glycopeptides from a trypsin digest of total protein extracts of Arabidopsis plants and release of these captured peptides following Peptide N-glycosidase (PNGase) treatment for analysis of N-glycan site-occupancy. The mixture of peptides released as a consequence of the PNGase treatment was analyzed by two dimensional nano-LC-MS. As the PNGase treatment of glycopeptides results in the deamidation of the asparagine (N) in the NxS/T site of the released peptide, this asparagine (N) to aspartic acid (D) conversion is used as a glycosylation 'signature'. The efficiency of PNGase F and PNGase A in peptide release is discussed. The identification of proteins with a single glycopeptide was limited by the used search algorithm but could be improved using a reference database including deamidated peptide sequences. Additional stringency settings were used for filtering results to minimize false discovery. This resulted in identification of 330 glycopeptides on 173 glycoproteins from Arabidopsis, of which 28 putative glycoproteins, that were previously not annotated as secreted protein in The Arabidopsis Information Resource database (TAIR). Furthermore, the identified glycosylation site occupancy helped to determine the correct topology for membrane proteins. A quantitative comparison of peptide signal was made between wild type and complex-glycan-less (cgl) mutant Arabidopsis from three replicate leaf samples using a label-free MS peak comparison. As an example, the identified membrane protein SKU5 (AT4G12420) showed differential glycopeptide intensity ratios between WT and cgl indicating heterogeneous glycan modification on single protein., Biological Significance: Proteins that enter the secretory pathway are mostly modified by N-glycans. The function of N-glycosylation has been well studied in mammals. However, in plants the function of N-glycosylation is still unclear, because glycosylation mutants in plants often do not have a clear phenotype. Here we analyzed which proteins are modified by N-glycans in plants by developing a glycopeptide enrichment method for plant proteins. Subsequently, label free comparative proteomics was employed using protein fractions from wild type and from a mutant which is blocked in modification of the N-glycan into complex glycans. The results provide new information on N-glycosylation sites on numerous secreted proteins. Results allow for specific mapping of multiple glycosylation site occupancy on proteins, which provides information on which glycosylation sites are protected or non-used from downstream processing and thus presumably are buried into the protein structure. Glycoproteomics can therefore contribute to protein structure analysis. Indeed, mapping the glycosylation sites on membrane proteins gives information on the topology of protein folds over the membrane. We thus were able to correct the topology prediction of three membrane proteins. Besides, these studies also identified limitations in the software that is used to identify single modified peptide per protein. This article is part of a Special Issue entitled: Translational Plant Proteomics., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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10. Alcohol drinking and endometrial cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) study.
- Author
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Fedirko V, Jenab M, Rinaldi S, Biessy C, Allen NE, Dossus L, Onland-Moret NC, Schütze M, Tjønneland A, Hansen L, Overvad K, Clavel-Chapelon F, Chabbert-Buffet N, Kaaks R, Lukanova A, Bergmann MM, Boeing H, Trichopoulou A, Oustoglou E, Barbitsioti A, Saieva C, Tagliabue G, Galasso R, Tumino R, Sacerdote C, Peeters PH, Bueno-de-Mesquita HB, Weiderpass E, Gram IT, Sanchez S, Duell EJ, Molina-Montes E, Arriola L, Chirlaque MD, Ardanaz E, Manjer J, Lundin E, Idahl A, Khaw KT, Romaguera-Bosch D, Wark PA, Norat T, and Romieu I
- Subjects
- Adult, Aged, Alcohol Drinking epidemiology, Alcoholic Beverages adverse effects, Confidence Intervals, Endometrial Neoplasms etiology, Europe epidemiology, Female, Humans, Incidence, Middle Aged, Odds Ratio, Proportional Hazards Models, Prospective Studies, Risk Factors, Socioeconomic Factors, Surveys and Questionnaires, Alcohol Drinking adverse effects, Endometrial Neoplasms epidemiology, Life Style
- Abstract
Purpose: Alcohol intake may adversely affect the concentrations of endogenous sex hormones, and thus increase the risk of endometrial cancer. However, epidemiologic studies have provided conflicting results. Therefore, we investigated the association between alcohol intake and endometrial cancer risk a large, multicenter, prospective study., Methods: From 1992 through 2010, 301,051 women in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort were followed for incident endometrial cancer (n = 1382). Baseline alcohol consumption was assessed by country-specific, validated dietary questionnaires. Information on past alcohol consumption was collected by lifestyle questionnaires. Hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated from Cox proportional hazard models., Results: The multivariable HRs (and 95% CIs) compared with light drinkers (0.1-6 g/d) were 1.03 (0.88-1.20) for 0 g of alcohol per day at baseline, 1.01 (0.86-1.17) for 6.1-12 g/d, 1.03 (0.87-1.22) for 12.1-24 g/d, 1.07 (0.87-1.38) for 24.1-36 g/d, and 0.85 (0.61-1.18) for more than 36 g/d (p(trend) = 0.77). No association was observed among former drinkers (OR, 1.28; 95% CI, 0.98-1.68 compared with light drinkers). Null associations were also found between alcohol consumption at age 20 years, lifetime pattern of alcohol drinking, and baseline alcohol intake from specific alcoholic beverages and endometrial cancer risk., Conclusions: Our findings suggest no association between alcohol intake and endometrial cancer risk., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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11. Message framing and medium considerations for recruiting newly eligible teen organ donor registrants.
- Author
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Quick BL, Bosch D, and Morgan SE
- Subjects
- Adolescent, Humans, Tissue Donors
- Abstract
In response to the current organ shortage-and in an effort to increase the number of registered donors-the present campaign employed a direct-mail marketing strategy that registered 6908 individuals in the state's First-Person Consent Registry (FPCR). In evaluating the most effective of three mailers, 18-year-old individuals (N = 139,356) were randomly assigned to receive: (a) a letter from the Secretary of State (SoS); (b) a brochure from the SoS or (c) both. As hypothesized, the results revealed that exposure to the SoS letter only resulted in a greater registration rate than exposure to the SoS brochure only. Results also revealed that exposure to both the SoS letter and SoS brochure resulted in a greater registration rate than exposure to the SoS brochure only. No difference in registration rate emerged between exposure to the SoS letter and SoS brochure compared to exposure to the SoS letter only. Our results speak to the effectiveness of utilizing personalized direct-mail marketing strategies to promote organ donation with an emphasis on the practical implications of our findings for organ donation practitioners., (© Copyright 2012 The American Society of Transplantation and the American Society of Transplant Surgeons.)
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- 2012
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12. N-glycoproteomics in plants: perspectives and challenges.
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Song W, Henquet MG, Mentink RA, van Dijk AJ, Cordewener JH, Bosch D, America AH, and van der Krol AR
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- Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Endoplasmic Reticulum metabolism, Glycopeptides metabolism, Glycoproteins metabolism, Glycosylation, Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase metabolism, Polysaccharides metabolism, Glycomics methods, Plant Proteins metabolism, Plants metabolism, Proteomics methods
- Abstract
In eukaryotes, proteins that are secreted into the ER are mostly modified by N-glycans on consensus NxS/T sites. The N-linked glycan subsequently undergoes varying degrees of processing by enzymes which are spatially distributed over the ER and the Golgi apparatus. The post-ER N-glycan processing to complex glycans differs between animals and plants, with consequences for N-glycan and glycopeptide isolation and characterization of plant glycoproteins. Here we describe some recent developments in plant glycoproteomics and illustrate how general and plant specific technologies may be used to address different important biological questions., (Copyright © 2011 Elsevier B.V. All rights reserved.)
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- 2011
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13. Synthesis of Lewis X epitopes on plant N-glycans.
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Rouwendal GJ, Florack DE, Hesselink T, Cordewener JH, Helsper JP, and Bosch D
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- Animals, Epitopes immunology, Fucosyltransferases genetics, Fucosyltransferases metabolism, Galactosyltransferases genetics, Galactosyltransferases metabolism, Humans, Plants, Genetically Modified genetics, Polysaccharides immunology, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Nicotiana genetics, Epitopes biosynthesis, Plants, Genetically Modified metabolism, Polysaccharides biosynthesis, Nicotiana metabolism
- Abstract
Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1-->4)-galactosyltransferase (GalT) and a hybrid alpha-(1-->3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1-->4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity.
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- 2009
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14. A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat.
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van den Broeck HC, America AH, Smulders MJ, Bosch D, Hamer RJ, Gilissen LJ, and van der Meer IM
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- Antigens, Plant isolation & purification, Flour analysis, Glutens isolation & purification, Humans, Plant Extracts chemistry, Plant Extracts isolation & purification, Triticum immunology, Antigens, Plant chemistry, Celiac Disease immunology, Chemical Fractionation methods, Glutens chemistry, Triticum chemistry
- Abstract
The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
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- 2009
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15. A CMP-sialic acid transporter cloned from Arabidopsis thaliana.
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Bakker H, Routier F, Ashikov A, Neumann D, Bosch D, and Gerardy-Schahn R
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- Animals, Arabidopsis Proteins genetics, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, Flow Cytometry, Nucleotide Transport Proteins genetics, Arabidopsis chemistry, Arabidopsis Proteins isolation & purification, Cytidine Monophosphate N-Acetylneuraminic Acid metabolism, Membrane Transport Proteins isolation & purification, Nucleotide Transport Proteins isolation & purification
- Abstract
Sialylation of glycans is ubiquitous in vertebrates, but was believed to be absent in plants, arthropods, and fungi. However, recently evidence has been provided for the presence of sialic acid in these evolutionary clades. In addition, homologs of mammalian genes involved in sialylation can be found in the genomes of these taxa and for some Drosophila enzymes, involvement in sialic acid metabolism has been documented. In plant genomes, homologs of sialyltransferase genes have been identified, but there activity could not be confirmed. Several mammalian cell lines exist with defects in the sialylation pathway. One of these is the Chinese hamster ovary cell line Lec2, deficient in CMP-sialic acid transport to the Golgi lumen. These mutants provide the possibility to clone genes by functional complementation. Using expression cloning, we have identified an Arabidopsis thaliana nucleotide sugar transporter that is able to complement the CMP-sialic acid transport deficiency of Lec2 cells. The isolated gene (At5g41760) is a member of the triose-phosphate/nucleotide sugar transporter gene family. Recombinant expression of the gene in yeast and testing in vitro confirmed its ability to transport CMP-sialic acid.
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- 2008
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16. Cerebral necrosis after 25Gy radiotherapy in childhood followed 28 years later by 54Gy radiotherapy.
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Koot RW, Stalpers LJ, Aronica E, and Andries Bosch D
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- Adult, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Child, Preschool, Combined Modality Therapy, Diagnosis, Differential, Follow-Up Studies, Gliosis pathology, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Meningeal Neoplasms surgery, Meningioma surgery, Necrosis, Neoplasms, Multiple Primary surgery, Neoplasms, Radiation-Induced surgery, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Radiotherapy, Adjuvant, Retreatment, Temporal Lobe pathology, Thallium Radioisotopes, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed, Cranial Irradiation adverse effects, Meningeal Neoplasms radiotherapy, Meningioma radiotherapy, Neoplasms, Multiple Primary radiotherapy, Neoplasms, Radiation-Induced radiotherapy, Precursor Cell Lymphoblastic Leukemia-Lymphoma radiotherapy, Radiation Injuries diagnosis, Temporal Lobe radiation effects
- Abstract
The development of brain necrosis is life-long risk of repeat radiation therapy, even after a long time interval and a moderate radiation dose. We report on a 34-year-old patient who had prophylactic cranial irradiation with 25Gy and adjuvant chemotherapy in childhood for leukaemia and in adulthood, 28 years later, therapeutic radiotherapy with 54Gy for an atypical (WHO grade II) meningioma. About 2 years later he developed a contrast-enhancing lesion on MRI-scan that was indicative of a tumor according to a thallium-201 ((201)Tl) SPECT scan. Histopathology of the operated contrast-enhancing lesion showed extensive radionecrosis. Radiation necrosis is a small but serious risk after repeat radiation therapy, even after a very long-term interval, the delivery of small fractions and an average cumulative total dose. Patients undergoing repeat radiotherapy therefore need to be followed life-long for potential late radiation toxicity.
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- 2007
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17. The contribution of plant foods to the vitamin A supply of lactating women in Vietnam: a randomized controlled trial.
- Author
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Khan NC, West CE, de Pee S, Bosch D, Phuong HD, Hulshof PJ, Khoi HH, Verhoef H, and Hautvast JG
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- Adult, Biological Availability, Carotenoids administration & dosage, Carotenoids pharmacokinetics, Female, Humans, Infant, Intestinal Absorption, Milk, Human drug effects, Nutritional Requirements, Nutritional Status, Rural Population, Vietnam, Vitamins administration & dosage, Vitamins pharmacokinetics, Fruit chemistry, Lactation metabolism, Milk, Human chemistry, Vegetables chemistry, Vitamin A administration & dosage, Vitamin A pharmacokinetics
- Abstract
Background: More information is needed on the efficacy of carotenoids from plant foods in improving vitamin A status., Objective: We aimed to quantify the efficacy of provitamin A-rich vegetables and fruit in improving vitamin A status., Design: Breastfeeding women in 9 rural communes in Vietnam were randomly allocated to 1 of 4 groups: the vegetable group (n = 73), which ingested 5.6 mg beta-carotene/d from green leafy vegetables; the fruit group (n = 69), which ingested 4.8 mg beta-carotene/d from orange or yellow fruit; the retinol-rich group (n = 70), which ingested 610 microg retinol/d from animal foods and 0.6 mg beta-carotene/d; and the control group (n = 68), which ingested 0.4 mg beta-carotene/d. Meals of groups 1, 2, and 4 contained <30 microg retinol/d. Lunch and dinner were provided 6 d/wk for 10 wk., Results: Mean (95% CI) changes in serum retinol concentrations of the vegetable, fruit, retinol-rich, and control groups were 0.09 (0.03, 0.16), 0.13 (0.07, 0.19), 0.25 (0.17, 0.33), and 0.00 (-0.06, 0.06) micromol/L, respectively. Mean (95% CI) changes in breast-milk retinol concentrations were 0.15 (0.04, 0.27), 0.15 (0.02, 0.28), 0.48 (0.32, 0.64), and -0.06 (-0.21, 0.09) micromol/L, respectively. According to these findings, the equivalent of 1 microg retinol would be 12 microg beta-carotene (95% CI: 8, 22 microg) for fruit and 28 microg beta-carotene (17, 84 microg) for green leafy vegetables. Thus, apparent mean vitamin A activity of carotenoids in fruit and in leafy vegetables was 50% (95% CI: 27%, 75%) and 21% (7%, 35%), respectively, of that assumed., Conclusion: The bioavailability of carotenoids from vegetables and fruit is less than previously assumed.
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- 2007
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18. Domain swapping of Citrus limon monoterpene synthases: impact on enzymatic activity and product specificity.
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El Tamer MK, Lücker J, Bosch D, Verhoeven HA, Verstappen FW, Schwab W, van Tunen AJ, Voragen AG, de Maagd RA, and Bouwmeester HJ
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- Amino Acid Sequence, Base Sequence, Bicyclic Monoterpenes, Bridged Bicyclo Compounds metabolism, Catalysis, Cyclohexane Monoterpenes, Cyclohexenes, Intramolecular Lyases genetics, Limonene, Models, Molecular, Molecular Sequence Data, Monoterpenes metabolism, Protein Conformation, Protein Structure, Tertiary physiology, Recombinant Proteins genetics, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Structure-Activity Relationship, Substrate Specificity, Terpenes metabolism, Citrus enzymology, Intramolecular Lyases chemistry, Intramolecular Lyases metabolism
- Abstract
Monoterpene cyclases are the key enzymes in the monoterpene biosynthetic pathway, as they catalyze the cyclization of the ubiquitous geranyl diphosphate (GDP) to the specific monoterpene skeletons. From Citrus limon, four monoterpene synthase-encoding cDNAs for a beta-pinene synthase named Cl(-)betaPINS, a gamma-terpinene synthase named ClgammaTS, and two limonene synthases named Cl(+)LIMS1 and Cl(+)LIMS2 were recently isolated [J. Lücker et al., Eur. J. Biochem. 269 (2002) 3160]. The aim of our work in this study was to identify domains within these monoterpene synthase enzymes determining the product specificity. Domain swapping experiments between Cl(-)betaPINS and ClgammaTS and between Cl(+)LIMS2 and ClgammaTS were conducted. We found that within the C-terminal domain of these monoterpene synthases, a region comprising 200 amino acids, of which 41 are different between Cl(-)betaPINS and ClgammaTS, determines the specificity for the formation of beta-pinene or gamma-terpinene, respectively, while another region localized further downstream is required for a chimeric enzyme to yield products in the same ratio as in the wild-type ClgammaTS. For Cl(+)LIMS2, the two domains together appear to be sufficient for its enzyme specificity, but many chimeras were inactive probably due to the low homology with ClgammaTS. Molecular modeling was used to further pinpoint the amino acids responsible for the differences in product specificity of ClgammaTS and Cl(-)betaPINS.
- Published
- 2003
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19. Plant-based raw material: improved food quality for better nutrition via plant genomics.
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van der Meer IM, Bovy AG, and Bosch D
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- Amino Acids biosynthesis, Esters metabolism, Flavonoids biosynthesis, Flavoring Agents, Genomics, Humans, Models, Chemical, Oligonucleotide Array Sequence Analysis methods, Quality Control, Food Technology methods, Genome, Plant, Nutritional Physiological Phenomena, Plants, Genetically Modified
- Abstract
Plants form the basis of the human food chain. Characteristics of plants are therefore crucial to the quantity and quality of human food. In this review, it is discussed how technological developments in the area of plant genomics and plant genetics help to mobilise the potential of plants to improve the quality of life of the rapidly growing world population.
- Published
- 2001
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20. GOA, a novel gene encoding a ring finger B-box coiled-coil protein, is overexpressed in astrocytoma.
- Author
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Vandeputte DA, Meije CB, van Dartel M, Leenstra S, IJlst-Keizers H, Das PK, Troost D, Bosch DA, Baas F, and Hulsebos TJ
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- Amino Acid Motifs, Amino Acid Sequence, Astrocytoma etiology, Astrocytoma genetics, Base Sequence, Brain metabolism, Chromosomes, Human, Pair 17, Gene Expression Profiling, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Molecular Sequence Data, RNA, Messenger biosynthesis, RNA, Neoplasm biosynthesis, Tissue Distribution, Astrocytoma metabolism, Carrier Proteins genetics, Carrier Proteins metabolism, Neoplasm Proteins, Nuclear Proteins genetics, Nuclear Proteins metabolism
- Abstract
Serial analysis of gene expression (SAGE) was used to identify a gene named GOA (gene overexpressed in astrocytoma), which codes for a novel Ring finger B-box coiled-coil (RBCC) protein. Northern blot hybridization showed overexpression of GOA in 9 of 10 astrocytomas. Except for kidney, in which high expression was found, expression levels in normal tissues were low and comparable to normal brain. Immunohistochemistry demonstrated presence of GOA, with prominent nuclear staining, in astrocytoma tumor cells and astrocytes of fetal brain, but virtual absence in mature astrocytes. Overexpression was not due to amplification, since amplification of GOA was only found in one of 65 astrocytomas. GOA was localized to 17q24-25, a region that is frequently gained or amplified in a number of other tumor types. GOA contains two LXXLL motifs, which are thought to be important for nuclear receptor binding. Our data suggest an important role of GOA in the process of dedifferentiation that is associated with astrocytoma tumorigenesis and possibly with that of other tumor types as well.
- Published
- 2001
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21. Equistatin, a protease inhibitor from the sea anemone actinia equina, is composed of three structural and functional domains.
- Author
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Strukelj B, Lenarcic B, Gruden K, Pungercar J, Rogelj B, Turk V, Bosch D, and Jongsma MA
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- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Recombinant Proteins chemistry, Sequence Alignment, Sequence Homology, Amino Acid, Proteins chemistry, Sea Anemones genetics, Serine Proteinase Inhibitors chemistry
- Abstract
A cDNA encoding a precursor of equistatin, a potent cysteine and aspartic proteinase inhibitor, was isolated from the sea anemone Actinia equina. The deduced amino acid sequence of a 199-amino-acid residue mature protein with 20 cysteine residues, forming three structurally similar thyroglobulin type-1 domains, is preceded by a typical eukaryotic signal peptide. The mature protein region and those coding for each of the domains were expressed in the periplasmic space of Escherichia coli, isolated, and characterized. The whole recombinant equistatin and its first domain, but not the second and third domains, inhibited the cysteine proteinase papain (K(i) 0.60 nM) comparably to natural equistatin. Preliminary results on inhibition of cathepsin D, supported by structural comparison, show that the second domain is likely to be involved in activity against aspartic proteinases., (Copyright 2000 Academic Press.)
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- 2000
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22. The thalamic contribution to the emergence of the readiness potential.
- Author
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Brunia CH, Bosch DA, Speelman JD, Van den Berg-Lenssen MM, and Van Boxtel GJ
- Subjects
- Aged, Essential Tremor diagnosis, Essential Tremor surgery, Hand physiology, Humans, Male, Middle Aged, Movement physiology, Parkinson Disease diagnosis, Thalamus surgery, Essential Tremor physiopathology, Evoked Potentials, Motor physiology, Parkinson Disease physiopathology, Thalamus physiology
- Published
- 2000
- Full Text
- View/download PDF
23. Unilateral pallidotomy in Parkinson's disease: a randomised, single-blind, multicentre trial.
- Author
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de Bie RM, de Haan RJ, Nijssen PC, Rutgers AW, Beute GN, Bosch DA, Haaxma R, Schmand B, Schuurman PR, Staal MJ, and Speelman JD
- Subjects
- Aged, Antiparkinson Agents administration & dosage, Antiparkinson Agents adverse effects, Female, Globus Pallidus physiopathology, Humans, Levodopa administration & dosage, Levodopa adverse effects, Male, Middle Aged, Neurologic Examination drug effects, Parkinson Disease diagnosis, Parkinson Disease physiopathology, Single-Blind Method, Stereotaxic Techniques, Treatment Outcome, Dominance, Cerebral physiology, Globus Pallidus surgery, Parkinson Disease surgery
- Abstract
Background: The results of several cohort studies suggest that patients with advanced Parkinson's disease would benefit from unilateral pallidotomy. We have assessed the efficacy of unilateral pallidotomy in a randomised, single-blind, multicentre trial., Methods: We enrolled 37 patients with advanced Parkinson's disease who had, despite optimum pharmacological treatment, at least one of the following symptoms: severe response fluctuations, dyskinesias, painful dystonias, or bradykinesia. Patients were randomly assigned to unilateral pallidotomy within 1 month or to pallidotomy after the primary outcome assessment (6 months later). The primary outcome was the difference between the groups in median changes on the motor examination section of the unified Parkinson's disease rating scale (UPDRS 3) score done in the off phase. Secondary outcome measures included levodopa-induced dyskinesias (dyskinesia rating scale [DRS]) and extent of disability (UPDRS 2)., Findings: The median UPDRS 3 off score of the pallidotomy patients improved from 47 to 32.5, whereas that of control patients slightly worsened from 52.5 to 56.5 (p<0.001). In the on phase the median DRS score improved 50% in pallidotomy patients compared with no change in controls. The UPDRS 2 off score improved with a median of 7 in the pallidotomy group. Two treated patients had major adverse effects., Interpretation: Unilateral pallidotomy is an effective treatment in patients with advanced Parkinson's disease, who have an unsatisfactory response to pharmacological treatment.
- Published
- 1999
- Full Text
- View/download PDF
24. An Arabidopsis thaliana cDNA complements the N-acetylglucosaminyltransferase I deficiency of CHO Lec1 cells.
- Author
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Bakker H, Lommen A, Jordi W, Stiekema W, and Bosch D
- Subjects
- Amino Acid Sequence, Animals, Arabidopsis genetics, Cloning, Molecular, Cricetinae, DNA, Complementary chemistry, Humans, Molecular Sequence Data, N-Acetylglucosaminyltransferases chemistry, N-Acetylglucosaminyltransferases deficiency, Sequence Alignment, Transfection, Arabidopsis enzymology, CHO Cells enzymology, DNA, Complementary genetics, Gene Expression, N-Acetylglucosaminyltransferases genetics
- Abstract
N-Acetylglucosaminyltransferase I (GlcNAcT-I, EC 2.4.1.101) is the enzyme which initiates the formation of complex N-linked glycans in eukaryotes by transforming GlcNAc to the oligo-mannosyl acceptor Man(5)GlcNAc(2)-Asn. The enzymatic activity and the structure that is synthesised by this enzyme are found in animals and plants but not in yeast. cDNAs encoding the enzyme have already been cloned from several mammals and the nematode Caenorhabditis elegans. In this article the cloning of an Arabidopsis thaliana GlcNAcT-I cDNA with homology to animal cDNAs is described. By expression of the plant cDNA in CHO Lec1 cells, a mammalian cell line deficient in GlcNAcT-I, it was shown that it encodes an active enzyme with the same enzymatic activity as the animal homologue. It has already been shown that a human GlcNAcT-I can complement an A. thaliana mutant (cgl-1). Here it is shown that the reverse is also true, the plant glycosyltransferase is able to complement a mammalian mutant (Lec1) deficient in GlcNAcT-I., (Copyright 1999 Academic Press.)
- Published
- 1999
- Full Text
- View/download PDF
25. Giant intracranial mucocele.
- Author
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van Manen SR, Bosch DA, Peeters FL, and Troost D
- Subjects
- Adult, Bone Neoplasms diagnosis, Bone Neoplasms pathology, Cerebrospinal Fluid Rhinorrhea, Cysts pathology, Cysts ultrastructure, Diagnosis, Differential, Ethmoid Sinus pathology, Frontal Lobe pathology, Humans, Magnetic Resonance Imaging, Male, Mucocele diagnosis, Mucocele surgery, Orbit pathology, Osteoblasts ultrastructure, Osteoma diagnosis, Osteoma pathology, Osteoma ultrastructure, Tomography, X-Ray Computed, Mucocele pathology
- Abstract
A 21-year-old man was admitted to hospital because of recent anosmia and liquorrhoea. He also complained of moderate headache and concentration problems in the past few years. On CT scan and MRI scans a big subfrontal process was seen, partially solid and partially cystic. Neurosurgical and histological findings proved that the lesion was an osteoma of the anterior skull base, concomitant with an intradurally extending mucocele.
- Published
- 1995
- Full Text
- View/download PDF
26. Ambulatory monitoring of tremor and other movements before and after thalamotomy: a new quantitative technique.
- Author
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van Someren EJ, van Gool WA, Vonk BF, Mirmiran M, Speelman JD, Bosch DA, and Swaab DF
- Subjects
- Adult, Aged, Aged, 80 and over, Algorithms, False Positive Reactions, Female, Humans, Male, Middle Aged, Monitoring, Physiologic instrumentation, Motor Activity physiology, Movement physiology, Postoperative Period, Time Factors, Tremor surgery, Wrist physiopathology, Ambulatory Care methods, Monitoring, Physiologic methods, Thalamus surgery, Tremor physiopathology
- Abstract
Tremor, e.g. in Parkinson patients, often shows large spontaneous fluctuations in severity over the day, to such an extent that a short observation is usually not sufficient to assess the overall severity or the effect of a treatment. Since momentary impressions of the tremor can thus be misleading, long-term ambulatory recordings would be helpful in the evaluation of severity and treatment effectiveness. As existing methods for long-term tremor registration have several shortcomings, a new method is proposed: an algorithm was designed to discriminate tremor from other movements and to describe the amount (i.e. the proportion of tremor or movements per time unit) as well as the intensity (i.e. average acceleration amplitude) of the two types of movement. In the evaluation of the severity of tremor both the amount and intensity of tremor episodes are of importance. The algorithm was tested on 24-h analog tape recordings of wrist-movement in 10 young and 10 aged controls, as well as in 8 patients with tremor--both before and after a tremor relieving thalamotomy. The algorithm scored movements as 'tremor' exclusively in patients prior to the operation. Fluctuations in tremor severity over the day were detected, and tremor could be discriminated from non-pathological movements. Moreover, following thalamotomy, motor slowing (bradykinesia) was detectable using this algorithm. Based on these test results, a miniaturized device in wrist-watch format is now being developed for long-term registrations.
- Published
- 1993
- Full Text
- View/download PDF
27. PAL-E, monoclonal antibody with immunoreactivity for endothelium specific to brain tumours.
- Author
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Leenstra S, Das PK, Troost D, Bosch DA, Claessen N, and Becker AE
- Subjects
- Humans, Antibodies, Monoclonal, Astrocytoma diagnosis, Brain Neoplasms diagnosis, Endothelium, Vascular analysis
- Published
- 1990
- Full Text
- View/download PDF
28. Organic psychosyndrome correlated with high density of grey matter on CT following metrizamide cervical myelography.
- Author
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Koppejan EH, Begeer N, Bosch DA, and Vencken LM
- Subjects
- Child, Humans, Male, Myelography adverse effects, Tomography, X-Ray Computed, Brain diagnostic imaging, Metrizamide adverse effects, Substance-Related Disorders etiology
- Published
- 1981
- Full Text
- View/download PDF
29. Assay for trenbolone and its metabolite 17 alpha-trenbolone in bovine urine based on immunoaffinity chromatographic clean-up and off-line high-performance liquid chromatography-thin-layer chromatography.
- Author
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van Ginkel LA, van Blitterswijk H, Zoontjes PW, van den Bosch D, and Stephany RW
- Subjects
- Animals, Cattle, Chromatography, Affinity, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Drug Implants, Gels, Immunoglobulin G analysis, Male, Spectrometry, Fluorescence, Trenbolone Acetate administration & dosage, Trenbolone Acetate adverse effects, Estrenes urine, Trenbolone Acetate urine
- Abstract
An high-performance liquid chromatography (HPLC)-thin-layer chromatography (TLC) method was developed to detect the illegal use of the xenobiotic growth promotor Trenbolone acetate (TBA). Very effective clean-up of bovine urine was achieved by immunoaffinity chromatography (IAC). The active form of TBA, the steroid 17 beta-Trenbolone (17 beta-TB), as well as its major metabolite 17 alpha-Trenbolone (17 alpha-TB), were assayed simultaneously with HPLC and on-line UV detection. The fraction containing 17 alpha-TB and 17 beta-TB (TB-fraction) was collected, and for confirmation 17 beta- and 17 alpha-TB were subsequently separated and identified by TLC. The limit of detection by on-line HPLC-UV (350 nm) was 1-2 micrograms TB/l. Off-line TLC detection was even more sensitive, 0.5 microgram 17 beta- or 17 alpha-TB/1. The assay was validated by investigating urine samples from veal calves implanted with TBA. The presence of 17 beta- and 17 alpha-TB was clearly demonstrated. A survey of the illegal use of TBA in cattle was performed by applying the assay to urine obtained at slaughter. No residues of TBA or its metabolites were found in any of the 144 random samples from the Dutch public health surveillance programme.
- Published
- 1988
- Full Text
- View/download PDF
30. Quantitative analysis of anabolics on thin-layer chromatographic plates using image analysis techniques.
- Author
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Jansen EH, van den Bosch D, Stephany RW, van Look LJ, and van Peteghem C
- Subjects
- Chromatography, Thin Layer, Image Processing, Computer-Assisted, Anabolic Agents analysis
- Abstract
A new detection system is introduced for the quantitative analysis of thin-layer chromatographic plates, which is based on a relatively simple, cheap but advanced image analysis system. Both one- and two-dimensional plates can be analysed. Recording and analysis can also be performed from photographs or even slides. Applications are shown for a number of samples containing anabolic compounds.
- Published
- 1989
- Full Text
- View/download PDF
31. Periplasmic accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12.
- Author
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Bosch D, Leunissen J, Verbakel J, de Jong M, van Erp H, and Tommassen J
- Subjects
- Autoradiography, Biological Transport, Electrophoresis, Polyacrylamide Gel, Genes, Bacterial, Immunochemistry, Mutation, Plasmids, Bacterial Outer Membrane Proteins metabolism, Escherichia coli metabolism
- Abstract
In order to localize the information within PhoE protein of Escherichia coli K-12 required for export of the protein to the outer membrane, we have generated deletions throughout the phoE gene. Immunocytochemical labelling on ultrathin cryosections revealed that the polypeptides encoded by the mutant alleles are transported to, and accumulate in, the periplasm. These results show that, except for the signal sequence, there is no specific sequence within the PhoE protein that is essential for transport through the cytoplasmic membrane. The overall structure of the protein, rather than a particular sequence of amino acids, seems to be important for assembly into the outer membrane.
- Published
- 1986
- Full Text
- View/download PDF
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