Search

Following experimental design, sample preparation for proteomic analyses is first critical step determining the overall quality of the proteomic output. A broad array of methods ranging from crude protein extraction through separations and sample clean-up techniques to sample enrichment have been discussed in this chapter. Brief assessment of pros and cons of these methods are provided. This chapter also guides the readers through the entire procedure from sample choice to analysis to method optimization.

Various types of electrophoretic separations are commonly used in proteomic approach. This chapter describes principle of electrophoresis in agarose, polyacrylamide, isoelectrofocusing, and 2-dimensional separations. Separations in native or denaturing conditions are discussed. This is followed by discussion of protein staining techniques for quantitation and visualization. Advantages and drawbacks are provided for the optimal choice of the most suitable method.