1. Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts.
- Author
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Hansen M, Varga E, Wüst T, Brouwer N, Beauchemin H, Mellink C, van der Kevie-Kersemaekers AM, Möröy T, van der Reijden B, von Lindern M, and van den Akker E
- Subjects
- Animals, Antigens, CD34 metabolism, Cell Line, Genetic Vectors genetics, Genetic Vectors metabolism, Hematopoietic Stem Cells cytology, Hematopoietic Stem Cells metabolism, Humans, Induced Pluripotent Stem Cells metabolism, Induced Pluripotent Stem Cells transplantation, Karyotype, Lentivirus genetics, Male, Megakaryocyte Progenitor Cells metabolism, Mice, Mice, Inbred NOD, Mice, SCID, Platelet Membrane Glycoprotein IIb metabolism, Teratoma metabolism, Teratoma pathology, Transcription Factors genetics, Transcription Factors metabolism, Cellular Reprogramming, Induced Pluripotent Stem Cells cytology, Megakaryocyte Progenitor Cells cytology
- Abstract
Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts., (Copyright © 2016. Published by Elsevier B.V.)
- Published
- 2017
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