1. Uniform water-mediated saturation transfer: A sensitivity-improved alternative to WaterLOGSY.
- Author
-
De Biasi F, Mascitti BB, Kupče Ē, and Rastrelli F
- Subjects
- Ligands, Macromolecular Substances, Magnetic Resonance Imaging, Proteins chemistry, Nanoparticles, Water
- Abstract
In the study of small molecule ligands and candidate macromolecular targets, water spins in long-lived association with macromolecules (proteins or nanoparticles) constitute a remarkable source of magnetization that can be exploited to reveal ligand-target binding. In this work we show how the selective saturation of water spins complemented with adiabatic off-resonance spin-locks can remove the NOE contribution of bulk water in the final difference spectrum, leading to uniformly enhanced signals that reveal weak ligand-target interactions., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Federico Rastrelli reports equipment, drugs, or supplies was provided by Italian Association for Cancer Research., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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