20 results on '"Aguilar, Zoraida P."'
Search Results
2. Nanomedical Devices
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Aguilar, Zoraida P., primary
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- 2013
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3. Biocompatibility and Functionalization
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Aguilar, Zoraida P., primary
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- 2013
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4. Nanopharmacology
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Aguilar, Zoraida P., primary
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- 2013
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5. Types of Nanomaterials and Corresponding Methods of Synthesis
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Aguilar, Zoraida P., primary
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- 2013
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6. Nanotoxicology and Remediation
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Aguilar, Zoraida P., primary
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- 2013
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7. Conclusions
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Aguilar, Zoraida P., primary
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- 2013
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8. Introduction
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Aguilar, Zoraida P., primary
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- 2013
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9. Acknowledgements
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Aguilar, Zoraida P., primary
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- 2013
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10. Hybrid RCA-DLS assay combined with aPCR for sensitive Salmonella enteritidis detection.
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Xie G, Zhan Z, Ye Y, Zhou B, Tong P, Aguilar ZP, and Xu H
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- DNA, Bacterial analysis, DNA, Bacterial genetics, Dynamic Light Scattering, Gold, Limit of Detection, Polymerase Chain Reaction methods, Metal Nanoparticles, Salmonella enteritidis genetics
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Salmonella infection could come from eating contaminated meat or raw eggs, and drinking milk or water contaminated by Salmonella enteritidis. Therefore, it is necessary to explore a fast and easy method for the detection of S. enteritidis in these diverse samples. For this purpose, a novel particle size sensing tool was designed for ultrasensitive and accurate S. enteritidis detection. This assay consisted of rolling circle amplification (RCA) with dynamic light scattering (DLS) using gold nanoparticles (AuNPs) modified with DNA probe as DNA-AuNPs as the capture surface into a hybrid RCA-DLS assay combined with asymmetric polymerase chain reaction (aPCR) and subsequent detection. Under optimal experimental conditions, the novel hybrid RCA-DLS assay combined with aPCR for S. enteritidis reached a limit of detection (LOD) as low as 3 × 10
0 CFU/mL in pure culture. In spiked milk samples, the LOD was 2.0 × 100 CFU/mL without pre-enriched bacteria. The total time of RCA-DLS assay was about 6 h which including genomic DNA extraction, aPCR, RCA and DLS determination. The hybrid RCA-DLS assay combined with aPCR holds promise in the specific and sensitive S. enteritidis detection., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2022
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11. Vancomycin-dendrimer based multivalent magnetic separation nanoplatforms combined with multiplex quantitative PCR assay for detecting pathogenic bacteria in human blood.
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Feng X, Meng X, Xiao F, Aguilar ZP, and Xu H
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- Bacteria, Humans, Magnetic Phenomena, Sensitivity and Specificity, Staphylococcus aureus genetics, Dendrimers, Vancomycin pharmacology
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Sepsis caused by bacteria has high morbidity and mortality, and it is neccerssay to establish a fast, convenient, and facility assays for detection of bacteria. In this study, we have developed established a simple, rapid, and ultrasensitive vancomycin (Van) and dendrimer nanoparticles-based method to isolate and detect bacteria in human blood using a multivalent binding strategy. The proposed Bio-den-Van multivalent capture nanoplatform combined with m-qPCR for simultaneous detection of two kinds of bacteria was demonstrated with rapid 2 min bacteria isolation with a linear range at 3.2 × 10
1 -3.2 × 106 CFU·mL-1 for L. monocytogenes and 4.1 × 101 -4.1 × 106 CFU·mL-1 for S. aureus, respectively. The limit of detection (LOD) for simultaneous detection of L. monocytogenes and S. aureus were 32 and 41 CFU·mL-1 in spiked human blood samples, respectively. Other bacteria had an insignificant interference with the test results. This Bio-den-Van multivalent capture nanoplatform combined with m-qPCR detection exhibited rapid, high sensitivity and specificity in simultaneous detection of various bacteria. To our knowledge, this is the first time that Bio-den-Van multivalent capture nanoplatform was used with Van as a recognition molecule for the simultaneous capture and subsequent detection of two bacteria from spiked human blood sample. This method holds great potential for future clinical applications., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2021
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12. Restraining the TiO 2 nanoparticles-induced intestinal inflammation mediated by gut microbiota in juvenile rats via ingestion of Lactobacillus rhamnosus GG.
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Zhao Y, Tang Y, Chen L, Lv S, Liu S, Nie P, Aguilar ZP, and Xu H
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- Adult, Animals, Child, Feces chemistry, Feces microbiology, Female, Homeostasis, Humans, Inflammation, Intestinal Mucosa immunology, Intestinal Mucosa metabolism, Intestinal Mucosa microbiology, Liver drug effects, Liver metabolism, Male, Nanoparticles metabolism, Rats, Titanium metabolism, Gastrointestinal Microbiome drug effects, Intestinal Mucosa drug effects, Lacticaseibacillus rhamnosus growth & development, Nanoparticles toxicity, Probiotics therapeutic use, Titanium toxicity
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Human were given a lot of opportunities to ingest TiO
2 NPs in the environment. Children have low, sensitive intestinal tolerance, and they could be exposed to higher levels of TiO2 NPs than adults. Few studies have been conducted on the interaction between TiO2 NPs and juvenile intestine phase models. Thus, in this work, weaning rats were orally exposed to TiO2 NPs for 7 and 14 days. Results indicate that Ti accumulated in the intestine, liver, and feces. Inflammatory infiltration damage was observed in the colonic epithelial tissue, and gut microbiota fluctuated with a decreased abundance of Lactobacilli in feces. Oral supplementation with Lactobacillus rhamnosus GG (LGG) lessened TiO2 NPs-induced colonic inflammatory injury, which might due to downregulation of nuclear factor kappa-B (NF-κB). Meanwhile, LGG maintained normal intestinal microbiome homeostasis, thereby improving TiO2 NPs-induced colon injury in juvenile rats. Moreover, fecal microbiota transplant (FMT) experiment indicated possible TiO2 NPs-induced intestinal microbiota disorder led to colonic inflammation. Our works suggested the urgent need for additional studies on the risk safety assessment, mechanism, and prevention of juvenile health damage from exposure to TiO2 NPs., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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13. Acute toxicity of quantum dots on late pregnancy mice: Effects of nanoscale size and surface coating.
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Zhang W, Yang L, Kuang H, Yang P, Aguilar ZP, Wang A, Fu F, and Xu H
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- Animals, Cadmium Compounds chemistry, Cadmium Compounds pharmacokinetics, Female, Fetus drug effects, Gene Expression Profiling, Gene Expression Regulation drug effects, Gonadal Steroid Hormones metabolism, Growth drug effects, Male, Mice, Organ Size drug effects, Particle Size, Placenta drug effects, Placenta metabolism, Pregnancy, Pregnancy Outcome, Uterus chemistry, Uterus metabolism, Cadmium Compounds toxicity, Pregnancy, Animal drug effects, Quantum Dots analysis, Quantum Dots toxicity
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In this study, the effects of cadmium containing QDs (such as CdSe/ZnS and CdSe QDs) and bulk CdCl2 in pregnant mice, their fetuses, and the pregnancy outcomes were investigated. It was shown that although the QDs and bulk CdCl2 were effectively blocked by the placental barrier, the damage on the placenta caused by CdSe QDs still led to fetus malformation, while the mice in CdSe/ZnS QDs treatment group exhibited slightly hampered growth but showed no significant abnormalities. Moreover, the Cd contents in the placenta and the uterus of CdSe QDs and CdSe/ZnS QDs treatment groups showed significantly higher than the CdCl2 treated group which indicated that the nanoscale size of the QDs allowed relative ease of entry into the gestation tissues. In addition, the CdSe QDs more effectively altered the expression levels of susceptive genes related to cell apoptosis, dysplasia, metal transport, cryptorrhea, and oxidative stress, etc. These findings suggested that the nanoscale size of the QDs were probably more important than the free Cd in inducing toxicity. Furthermore, the results indicated that the outer surface shell coating played a protective role in the adverse effects of QDs on late pregnancy mice., (Copyright © 2016 Elsevier B.V. All rights reserved.)
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- 2016
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14. Size dependent effect of ZnO nanoparticles on endoplasmic reticulum stress signaling pathway in murine liver.
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Kuang H, Yang P, Yang L, Aguilar ZP, and Xu H
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- Administration, Oral, Animals, Biomarkers blood, Endoplasmic Reticulum Stress genetics, Female, Gene Expression drug effects, Liver metabolism, Liver pathology, Mice, Inbred Strains, Nanoparticles analysis, Particle Size, Zinc Oxide analysis, Endoplasmic Reticulum Stress drug effects, Liver drug effects, Nanoparticles toxicity, Zinc Oxide toxicity
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ZnO nanoparticles (NPs) have been assessed to show adverse effects on the liver, but the molecular mechanisms and the role of nanoparticle properties in these adverse reactions have not been sufficiently studied. In this study, the toxicity of various sizes of ZnO particles (bulk, 90nm, and 30nm) that were ingested orally over a period of 3days were evaluated in mice. The blood biochemistry, hematological analyses, and histopathological evaluation showed that there was apparent toxicity caused by smaller ZnO NPs (30nm) in liver. The smallest ZnO NPs showed highest accumulation in the mice liver. The RT-qPCR data indicated that 30nm ZnO NPs can induce significant endoplasmic reticulum (ER) stress responses. The ER stress marker of PERK, eIF2α, ATF4, Chop, JNK, caspase-12, caspase-9, GRP94, and Bax at the mRNA levels were higher expression in 30nm ZnO NP than that in bulk or 90nm ZnO. These findings implied that the smaller ZnO NPs (30nm) activated ER stress responses that signified severe apoptosis in murine liver., (Copyright © 2016 Elsevier B.V. All rights reserved.)
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- 2016
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15. Sulfonated polystyrene magnetic nanobeads coupled with immunochromatographic strip for clenbuterol determination in pork muscle.
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Wu K, Guo L, Xu W, Xu H, Aguilar ZP, Xu G, Lai W, Xiong Y, and Wan Y
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- Adsorption, Animals, Chromatography, Liquid methods, Hydrogen-Ion Concentration, Limit of Detection, Magnetics, Mass Spectrometry methods, Microspheres, Nanoparticles, Reproducibility of Results, Solid Phase Extraction, Swine, Temperature, Chromatography, Affinity methods, Clenbuterol analysis, Drug Residues analysis, Food Contamination analysis, Meat analysis, Polystyrenes chemistry
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A magnetic solid-phase extraction method (MSPE) was developed to pre-concentrate and cleanup clenbuterol (CLE) from pork muscle. Novel sulfonated polystyrene magnetic nanobeads (spMNBs) were synthesized via a one-pot emulsion copolymerization method by using divinylbenzene, styrene, and sodium styrene sulfonate in the presence of oleic acid-modified and 10-undecylenic acid-modified magnetic ferrofluid. The resulting spMNBs exhibited high adsorption efficiency for CLE and for 10 other common beta-adrenergic agonists, namely, brombuterol, ractopamine, tulobuterol, bambuterol, cimbuterol, mabuterol, clorprenaline, penbutolol, salbutamol, and cimaterol. The adsorption behavior of the spMNBs for CLE was described by the Langmuir equation with a maximum adsorption capacity of 0.41 mg/g. Under the optimized parameters, the extraction of CLE from 0.5 g of pork muscle required 25mg of the spMNBs at a shortened adsorption time (0.5 min). The proposed MSPE was coupled with colloidal gold nanoparticle-based immunochromatographic assay (MSPE-AuNPIA) for the quantitative detection of CLE residue in pork muscle. The limit of detection and limit of quantification for the pork muscle were 0.10 and 0.24 ng/g, respectively. The intra-day and inter-day assay recoveries at three CLE spiked concentrations ranged from 92.5% to 98.1%, with relative standard deviations ranging from 3.2% to 13.0%. The results of MSPE-AuNPIA were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The CLE values obtained with MSPE-AuNPIA agreed with those obtained with LC-MS/MS., (Copyright © 2014 Elsevier B.V. All rights reserved.)
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- 2014
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16. A gold@silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection.
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Al-Ogaidi I, Gou H, Al-Kazaz AK, Aguilar ZP, Melconian AK, Zheng P, and Wu N
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- Ascorbic Acid chemistry, Enzymes, Immobilized chemistry, Enzymes, Immobilized metabolism, Gluconates analysis, Glucose Oxidase chemistry, Glucose Oxidase metabolism, Hydrogen Peroxide analysis, Oxidation-Reduction, Uric Acid chemistry, Biosensing Techniques, Chemistry Techniques, Analytical instrumentation, Glucose analysis, Gold chemistry, Metal Nanoparticles chemistry, Silicon Dioxide chemistry, Spectrum Analysis, Raman
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The gold nanostar@silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 μM to 25 mM in the aqueous solution with the limit of detection of 16 μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample., (Copyright © 2013 Elsevier B.V. All rights reserved.)
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- 2014
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17. Antibacterial activity and mechanism of action of ε-poly-L-lysine.
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Ye R, Xu H, Wan C, Peng S, Wang L, Xu H, Aguilar ZP, Xiong Y, Zeng Z, and Wei H
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- Acetylcysteine pharmacology, Azides pharmacology, Cell Membrane metabolism, DNA Damage, Dose-Response Relationship, Drug, Escherichia coli O157 metabolism, Food Contamination prevention & control, Food Microbiology, Gene Expression Regulation, Bacterial, Microscopy, Atomic Force, Microscopy, Electron, Transmission, Oxidation-Reduction, Oxidative Stress, Propidium analogs & derivatives, Propidium pharmacology, Reactive Oxygen Species metabolism, Anti-Bacterial Agents pharmacology, Escherichia coli O157 drug effects, Polylysine pharmacology
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ε-Poly-L-lysine (ε-PL)(2) is widely used as an antibacterial agent because of its broad antimicrobial spectrum. However, the mechanism of ε-PL against pathogens at the molecular level has not been elucidated. This study investigated the antibacterial activity and mechanism of ε-PL against Escherichia coli O157:H7 CMCC44828. Propidium monoazide-PCR test results indicated that the threshold condition of ε-PL for complete membrane lysis of E. coli O157:H7 was 10 μg/mL (90% mortality for 5 μg/mL). Further verification of the destructive effect of ε-PL on cell structure was performed by atomic force microscopy and transmission electron microscopy. Results showed a positive correlation between reactive oxygen species (ROS)(3) levels and ε-PL concentration in E. coli O157:H7 cells. Moreover, the mortality of E. coli O157:H7 was reduced when antioxidant N-acetylcysteine was added. Results from real-time quantitative PCR (RT-qPCR)(4) indicated that the expression levels of oxidative stress genes sodA and oxyR were up-regulated 4- and 16-fold, respectively, whereas virulence genes eaeA and espA were down-regulated after ε-PL treatment. Expression of DNA damage response (SOS response)(5) regulon genes recA and lexA were also affected by ε-PL. In conclusion, the antibacterial mechanism of ε-PL against E. coli O157:H7 may be attributed to disturbance on membrane integrity, oxidative stress by ROS, and effects on various gene expressions, such as regulation of oxidative stress, SOS response, and changes in virulence., (Copyright © 2013. Published by Elsevier Inc.)
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- 2013
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18. Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in food products.
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Yang Y, Xu F, Xu H, Aguilar ZP, Niu R, Yuan Y, Sun J, You X, Lai W, Xiong Y, Wan C, and Wei H
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- Animals, Azides pharmacology, Bacterial Typing Techniques, Cattle, Escherichia coli O157 drug effects, Escherichia coli O157 genetics, Escherichia coli O157 growth & development, Listeria monocytogenes drug effects, Listeria monocytogenes genetics, Listeria monocytogenes growth & development, Microbial Viability, Multiplex Polymerase Chain Reaction instrumentation, Propidium analogs & derivatives, Propidium pharmacology, Salmonella typhimurium drug effects, Salmonella typhimurium genetics, Salmonella typhimurium growth & development, Escherichia coli O157 isolation & purification, Lactuca microbiology, Listeria monocytogenes isolation & purification, Solanum lycopersicum microbiology, Meat microbiology, Multiplex Polymerase Chain Reaction methods, Salmonella typhimurium isolation & purification
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We developed a rapid and reliable technique for simultaneous detection of Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes that can be used in food products. Magnetic nano-beads (MNBs) based immunomagnetic separation (IMS) was used to separate the target bacterial cells while multiplex PCR (mPCR) was used to amplify the target genes. To detect only the viable bacteria, propidium monoazide (PMA) was applied to selectively suppress the DNA detection from dead cells. The results showed the detection limit of IMS-PMA-mPCR assay was about 10(2) CFU/ml (1.2 × 10(2) CFU/ml for S. Typhimurium, 4.0 × 10(2) CFU/ml for E. coli O157:H7 and 5.4 × 10(2) CFU/ml for L. monocytogenes) in pure culture and 10(3) CFU/g (5.1 × 10(3) CFU/g for S. Typhimurium, 7.5 × 10(3) CFU/g for E. coli O157:H7 and 8.4 × 10(3) CFU/g for L. monocytogenes) in spiking food products samples (lettuce, tomato and ground beef). This report has demonstrated for the first time, the effective use of rapid and reliable IMS combined with PMA treatment and mPCR assay for simultaneous detection of viable S. Typhimurium, E. coli O157:H7 and L. monocytogenes in spiked food samples. It is anticipated that the present approach will be applicable to simultaneous detection of the three target microorganisms for practical use., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
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- 2013
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19. Identification of an outer membrane protein of Salmonella enterica serovar Typhimurium as a potential vaccine candidate for Salmonellosis in mice.
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Yang Y, Wan C, Xu H, Aguilar ZP, Tan Q, Xu F, Lai W, Xiong Y, and Wei H
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- Adhesins, Bacterial genetics, Animals, Bacterial Adhesion, Bacterial Outer Membrane Proteins genetics, Cell Line, Disease Models, Animal, Epithelial Cells microbiology, Female, Humans, Mice, Mice, Inbred BALB C, Salmonella Infections, Animal immunology, Salmonella Infections, Animal mortality, Salmonella Infections, Animal pathology, Salmonella Vaccines administration & dosage, Salmonella Vaccines genetics, Salmonella typhimurium genetics, Salmonella typhimurium physiology, Survival Analysis, Vaccines, Subunit administration & dosage, Vaccines, Subunit genetics, Vaccines, Subunit immunology, Vaccines, Synthetic administration & dosage, Vaccines, Synthetic genetics, Vaccines, Synthetic immunology, Adhesins, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Salmonella Infections, Animal prevention & control, Salmonella Vaccines immunology, Salmonella typhimurium immunology
- Abstract
We report our investigation of the functions of PagN in Salmonella pathogenesis and its potential as a vaccine candidate. Further investigation conducted in this study indicates that the outer membrane protein PagN is important for Salmonella adhesion/invasion of epithelial cells as well as bacterial virulence. When pagN was deleted from Salmonella enterica serovar Typhimurium (S. Typhimurium), the adhesion and invasion of HT-29 epithelial cells was significantly decreased compared with the wild type strain. Mice infected with the pagN mutant strain exhibited less pathological signs in the intestine and survived longer than the wild-type-infected mice. PagN is widely distributed and conserved among clinical isolates of different Salmonella serovars, making PagN a potential vaccine candidate for Salmonella infection. To elucidate the potential of PagN as a vaccine, we expressed and purified recombinant PagN (rPagN). When rPagN was tested in mice, it provided significant protection against Salmonella infection in vivo. In vitro, anti-PagN serum enhanced clearance of Salmonella, indicating a contribution of PagN-specific antibodies to the killing process. This correlates well with the observed protection of mice immunized with rPagN. Our preliminary results indicate more functions of PagN in S. Typhimurium virulence as well as its potential as a protective vaccine., (Copyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
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- 2013
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20. Nanobeads-based rapid magnetic solid phase extraction of trace amounts of leuco-malachite green in Chinese major carps.
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Guo L, Zhang J, Wei H, Lai W, Aguilar ZP, Xu H, and Xiong Y
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- Acetonitriles chemistry, Adsorption, Analytic Sample Preparation Methods, Animals, Hydrogen-Ion Concentration, Osmolar Concentration, Rosaniline Dyes chemistry, Time Factors, Carps, Food Contamination analysis, Magnets chemistry, Nanoparticles chemistry, Rosaniline Dyes analysis, Rosaniline Dyes isolation & purification, Solid Phase Extraction methods
- Abstract
A proof-of-concept for the use of oleic acid coated magnetic nanobeads (OA-MNBs) for the magnetic solid phase extraction (MSPE) of trace amounts of leuco-malachite green (LMG) from fish samples was developed. The OA-MNBs were prepared by covalently conjugating oleic acid on amino-modified magnetic polystyrene beads. The OA-MNBs were characterized with transmission electron microscopy, Fourier transform infrared spectroscopy and zeta-potential analyzer. The optimized parameters for MSPE with OA-MNBs of LMG from fish muscle involved a combination of pH 10.0 in 10% acetonitrile, 1.5 M sodium chloride as an adsorption solution, and an extraction procedure involving 6 mg OA-MNBs in 18 mL LMG adsorption solution. This was optimized for 0.5 g fish muscles with an incubation period of 10 min using 200 μL acetonitrile for elution. Using the optimized parameters, the performance of MSPE with OA-MNBs was evaluated by analyzing LMG-spiked fish extracts with liquid chromatography-mass spectrometry (LC-MS/MS) method. The results indicated that recoveries of LMG (from 0.1 to 2 ng/g) ranged from 71.2%-112.6% with relative standard deviations as low as 0.6%. Out of 57 field fish samples, eight LMG positive samples were confirmed using MSPE with OA-MNBs. Compared with traditional liquid-liquid extraction methods, the results showed that MSPE with OA-MNBs had a higher sensitivity for samples with low LMG concentration. Furthermore, the MSPE with OA-MNB took only 40 min to perform without the need for time consuming sample-pretreatment process. Therefore, MSPE with OA-MNBs holds promise for rapid, sensitive, and cost effective screening for LMG in fish samples., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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