1. Increased intramuscular nerve branching and inhibition of programmed cell death of chick embryo motoneurons by immunoglobulins from patients with motoneuron disease
- Author
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Carol Milligan, Ricardo Rojas, James B. Caress, Dolors Ciutat, Carles Solsona, Laura Texidó, David Prevette, Joan Blasi, Sara Hernández, Lídia Piedrafita, Mònica Povedano, Anna Casanovas, Isabel Illa, Ronald W. Oppenheim, Josep E. Esquerda, and Jordi Calderó
- Subjects
Male ,Proteomics ,Serum ,Statistics as Topic ,Apoptosis ,Chick Embryo ,Semaphorins ,Cerebrospinal fluid ,Tubulin ,Ganglia, Spinal ,Chlorocebus aethiops ,Immunology and Allergy ,Electrophoresis, Gel, Two-Dimensional ,Amyotrophic lateral sclerosis ,Cells, Cultured ,Programmed cell death ,Motor Neurons ,Embryo ,Motoneuron ,Neurology ,embryonic structures ,Female ,Antibody ,animal structures ,Cell Survival ,Immunology ,Growth Cones ,Neuromuscular Junction ,Immunoglobulins ,Enzyme-Linked Immunosorbent Assay ,Biology ,In Vitro Techniques ,In ovo ,Transfection ,Statistics, Nonparametric ,Andrology ,Semaphorin ,medicine ,Animals ,Humans ,Motor Neuron Disease ,Muscle, Skeletal ,Analysis of Variance ,Dose-Response Relationship, Drug ,medicine.disease ,Embryonic stem cell ,Molecular biology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Chick embryo ,Neurology (clinical) - Abstract
Massive programmed cell death (PCD) of developing chick embryo motoneurons (MNs) occurs in a well defined temporal and spatial sequence between embryonic day (E) 6 and E10. We have found that, when administered in ovo, either circulating immunoglobulins G (IgGs) or cerebrospinal fluid from patients with MN disease can rescue a significant number of chick embryo MNs from normally occurring PCD. An increase of branching of intramuscular nerves was also observed that may account for the rescuing effects of pathologic IgGs. Proteomic analysis and further analysis by ELISA indicated that these effects may be mediated by the interaction of circulating human immunoglobulins with proteins of the semaphorin family. (C) 2010 Elsevier B.V. All rights reserved.
- Published
- 2010