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Summary: Complement-dependent microglia pruning of excitatory synapses has been widely reported in physiological and pathological conditions, with few reports concerning pruning of inhibitory synapses or direct regulation of synaptic transmission by complement components. Here, we report that loss of CD59, an important endogenous inhibitor of the complement system, leads to compromised spatial memory performance. Furthermore, CD59 deficiency impairs GABAergic synaptic transmission in the hippocampal dentate gyrus (DG). This depends on regulation of GABA release triggered by Ca2+ influx through voltage-gated calcium channels (VGCCs) rather than inhibitory synaptic pruning by microglia. Notably, CD59 colocalizes with inhibitory pre-synaptic terminals and regulates SNARE complex assembly. Together, these results demonstrate that the complement regulator CD59 plays an important role in normal hippocampal function.

Chitosan/MOF composite porous monolith used in water remediation as adsorbent can realize high-efficient removal of pollutant in water and facile recycling from water. However, dissolution of chitosan (without crosslinking) in acidic aqueous solution will cause breakage of composite monolith. Herein, we report a chitosan/UiO-66 monolith prepared by ice-templating method. Specially, a pre-crosslinking treatment (by glutaraldehyde) is employed before the monolith formation, which obviously boosts its stability in aqueous solution. The composite monolith is evaluated by SEM, N2 adsorption, XRD, and batch adsorption tests for Congo Red (CR). The results show that the composite monolith possesses a typical ice-templating structure with hierarchical (mirco- / meso- and macro-) pores. UiO-66 particles are embedded on the surface of chitosan matrix, and the crystal structure of UiO-66 is not changed obviously by the crosslinking and freezing process. The composite monolith exhibits high adsorption efficiency (90% of CR was removed from its aqueous solution in 60 min) and the maximum adsorption capacity of 246.21 mg/g (derived from Langmuir model) can be reached. After adsorption, the monolith is collected by a facile procedure and recovered using ethanol for evaluating its reusability. After 4 cycles, the CR removal efficiency of the composite monolith still remains ~90% of the initial efficiency. This work demonstrates that the simple crosslinking procedure before monolith formation can ensure the intact shape of the chitosan/MOF monolith during adsorption.