1. Cells carrying C5b-9 complement complexes in human atherosclerotic wall.
- Author
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Rus HG, Niculescu F, Poruţiu D, Ghiurca V, and Vlaicu R
- Subjects
- Antibodies, Monoclonal, Complement Membrane Attack Complex, Femoral Artery immunology, Femoral Artery ultrastructure, Humans, Iliac Artery immunology, Iliac Artery ultrastructure, Macrophages cytology, Arteriosclerosis immunology, Complement System Proteins immunology
- Abstract
Fibrous plaques and intimal thickenings of 5 femoral and 5 iliac human arteries obtained at surgery were processed for indirect and double-labeling immunoelectron microscopy using an affinity purified rabbit IgG anti-C5b-9 neoantigen and the EBM 11 monoclonal antibody anti-human macrophages. The C5b-9 complexes were localized in intact cells, disintegrated cells and cell debris enmeshed in the connective tissue matrix. Some of the cell debris bearing C5b-9 deposits was found to be of macrophage origin. Endocyted or exocyted pieces of membrane with pore-forming C5b-9 complexes were also identified. Damage of cells by complement in atherosclerotic lesions may contribute to atherogenesis.
- Published
- 1989
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