1. Exosome-shuttled miR-92b-3p from ischemic preconditioned astrocytes protects neurons against oxygen and glucose deprivation.
- Author
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Xu L, Cao H, Xie Y, Zhang Y, Du M, Xu X, Ye R, and Liu X
- Subjects
- Animals, Apoptosis drug effects, Brain Ischemia metabolism, Cell Hypoxia drug effects, Cell Survival drug effects, Embryo, Mammalian, Exome physiology, Female, Glucose metabolism, Ischemic Preconditioning methods, Male, MicroRNAs genetics, Neurons metabolism, Neuroprotection drug effects, Neuroprotective Agents pharmacology, Oxygen metabolism, Rats, Rats, Sprague-Dawley, Signal Transduction drug effects, Astrocytes metabolism, Brain Ischemia genetics, MicroRNAs metabolism
- Abstract
Ischemic preconditioning (IPC) exerts protective effects against ischemic cerebral injury. In the present study, an in vitro model of cerebral ischemia (oxygen and glucose deprivation, OGD) was established to investigate the neuroprotective mechanism of IPC. We found that conditioned medium (C.M.) from astrocytes rather than neurons nor microglia cell line BV2 exerted neuroprotection. Moreover, exosomes derived from OGD preconditioned astrocytes can be taken up by neurons and attenuated OGD-induced neuron death and apoptosis. High-throughput microRNA (miRNA) sequencing revealed that miR-92b-3p levels in exosomes released from preconditioned astrocytes were increased. Overexpression of miR-92b-3p in neurons with miR-92b-3p mimic achieved the same protective effects as C.M. from astrocytes. Thus, we propose that the mechanism of IPC may associate with astrocytes, and that exosome-mediated miR-92b-3p shuttle from preconditioned astrocytes to neurons participate in these process., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2019
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