1. Physical and genetic characterization of the genome of Magnetospirillum magnetotacticum, strain MS-1.
- Author
-
Bertani LE, Weko J, Phillips KV, Gray RF, and Kirschvink JL
- Subjects
- Amino Acid Sequence, Base Sequence, Carrier Proteins genetics, Cytochrome b Group genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Circular genetics, Electrophoresis, Gel, Pulsed-Field, Ferritins genetics, Gene Order, Membrane Proteins genetics, Molecular Sequence Data, Protein Subunits, Restriction Mapping, Sequence Analysis, DNA, Bacterial Proteins, Cation Transport Proteins, Genome, Bacterial, Rhodospirillaceae genetics
- Abstract
Pulsed-field gel analysis of Magnetospirillum magnetotacticum, strain MS-1, indicates that the genome is a single, circular structure of about 4.3 mb. A few genes, identified by sequence similarity, have been localized and arranged in a map with dnaA, indicating the presumed origin of replication. There are at least two rRNA operons. In addition, rRNA genes are found on a 40 kb, possibly extrachromosomal, structure. The genes thought to be involved in magnetite synthesis, bfr and magA, are located in the same 17% of the genome. A one base pair-overlap seen in the bfr genes of MS-1 is found also in the closely related magnetic strain AMB-1, but not in the non-magnetic relative A. itersonii.
- Published
- 2001
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