1. cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope.
- Author
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Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, and Kawasaki T
- Subjects
- 5' Flanking Region genetics, Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Chromosome Mapping, Cloning, Molecular, DNA, Complementary chemistry, Exons, Gene Expression Regulation, Enzymologic, Genes genetics, Glucuronosyltransferase metabolism, Introns, Isoenzymes genetics, Isoenzymes metabolism, Mice, Mice, Inbred BALB C, Molecular Sequence Data, RNA, Messenger genetics, RNA, Messenger metabolism, Regulatory Sequences, Nucleic Acid genetics, Sequence Analysis, DNA, Transcription Initiation Site, CD57 Antigens metabolism, DNA, Complementary genetics, Epitopes biosynthesis, Glucuronosyltransferase genetics
- Abstract
The HNK-1 carbohydrate epitope is expressed on a series of cell adhesion molecules and some glycolipids in the nervous system. Two glucuronyltransferases (GlcAT-P and GlcAT-S) are involved in the biosynthesis of the HNK-1 carbohydrate epitope. In this study, we isolated cDNA and genomic clones encoding the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope and determined the structural organization of the gene. The deduced amino acid sequence of mouse GlcAT-S consists of 324 amino acids and has a type II membrane topology. The predicted amino acid sequence of mouse GlcAT-S is 98.1% identical to that of rat GlcAT-S. Northern blot analysis revealed that the mouse GlcAT-S transcript is specifically expressed in the nervous system. Moreover, the mouse GlcAT-S gene is composed of four exons spanning over more than 25 kilobase pairs. Southern blot analysis and chromosomal mapping indicated that the mouse GlcAT-S gene is a single copy gene and it was mapped to the A4-B region of mouse chromosome 1.
- Published
- 2002
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