1. CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.
- Author
-
Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, and Mallet JM
- Subjects
- Animals, Bacterial Proteins genetics, Bacterial Proteins metabolism, Calcium chemistry, Calcium Signaling, Color, Indicators and Reagents chemical synthesis, Luminescent Measurements methods, Luminescent Proteins genetics, Luminescent Proteins metabolism, Maleimides chemistry, Membrane Potentials, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Fluorescence, Multiphoton methods, Models, Chemical, Molecular Structure, Neurons metabolism, Neurons physiology, Reproducibility of Results, Calcium analysis, Fluorescent Dyes chemistry, Indicators and Reagents chemistry, Neurons chemistry
- Abstract
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
- Published
- 2015
- Full Text
- View/download PDF