4 results on '"Stephen J. Blanksby"'
Search Results
2. No turnover in lens lipids for the entire human lifespan
- Author
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Roger J.W. Truscott, Jessica R Hughes, Alan Williams, Stephen J. Blanksby, Vladimir Levchenko, and Todd W. Mitchell
- Subjects
lens ,QH301-705.5 ,Science ,Lipid composition ,Membrane lipids ,Short Report ,Biology ,General Biochemistry, Genetics and Molecular Biology ,lipids ,03 medical and health sciences ,Membrane Lipids ,0302 clinical medicine ,lipid ,Lens, Crystalline ,medicine ,Humans ,C14 ,Carbon Radioisotopes ,human ,Biology (General) ,Eye Proteins ,Human Biology and Medicine ,Lens crystalline ,030304 developmental biology ,mass spectrometry ,0303 health sciences ,General Immunology and Microbiology ,General Neuroscience ,turnover ,Correction ,Lipid metabolism ,General Medicine ,Cell Biology ,Lipid Metabolism ,Cell biology ,medicine.anatomical_structure ,Biochemistry ,Lens cell ,Lens (anatomy) ,Medicine ,Eye disorder ,030217 neurology & neurosurgery ,Function (biology) - Abstract
Lipids are critical to cellular function and it is generally accepted that lipid turnover is rapid and dysregulation in turnover results in disease (Dawidowicz 1987; Phillips et al., 2009; Liu et al., 2013). In this study, we present an intriguing counter-example by demonstrating that in the center of the human ocular lens, there is no lipid turnover in fiber cells during the entire human lifespan. This discovery, combined with prior demonstration of pronounced changes in the lens lipid composition over a lifetime (Hughes et al., 2012), suggests that some lipid classes break down in the body over several decades, whereas others are stable. Such substantial changes in lens cell membranes may play a role in the genesis of age-related eye disorders. Whether long-lived lipids are present in other tissues is not yet known, but this may prove to be important in understanding the development of age-related diseases. DOI: http://dx.doi.org/10.7554/eLife.06003.001, eLife digest Every cell is surrounded by a membrane made primarily of molecules called lipids. This membrane protects the cell and controls which molecules pass into and out of it. To keep the membrane in good working order, its lipids are regularly broken down and replaced with fresh molecules. However, some cells—such as the cells that make up most of the lens of the eye—lack easy access to the cell machinery that renews the membrane. The lens grows throughout life by adding new cells to the outside of the lens, but the center of the lens—also known as the lens nucleus—contains the same cells that were present at birth. This raises the question of whether the lipids in the membranes of these cells also remain in the cells for life. From 1955 to 1963, above-ground test explosions of nuclear weapons caused a large amount of a radioactive form of carbon called carbon-14 to be released into the atmosphere. In subsequent years, these levels have decreased again as the carbon-14 is absorbed into the oceans or incorporated into biological molecules—like lipids. This doesn't affect the molecules, as carbon-14 works just like normal carbon. However, as the proportion of carbon-14 in a group of molecules reflects the amount of carbon-14 in the atmosphere when the molecule was made, this allows the age of the molecule to be determined. Hughes et al. used a technique called mass spectrometry to measure the carbon-14 in lens nuclei donated by 23 people who were born between 1948 and 1993. This revealed that the proportion of carbon-14 in the total carbon content of the lipids in the nucleus could be used to accurately predict the year of birth of the donor. Therefore, the lipids in your lenses when you are born remain with you for your entire life. This finding could help us to understand age-related sight disorders, such as cataracts. Further research could also investigate whether there are any similarly long-lasting lipids in other body tissues, and whether these affect how other age-related diseases develop. DOI: http://dx.doi.org/10.7554/eLife.06003.002
- Published
- 2015
3. Sex-specific triacylglycerides are widely conserved in Drosophila and mediate mating behavior
- Author
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Qi Ling Koh, Shane R. Ellis, Stephen J. Blanksby, William J. Etges, Huong T. Pham, Kenji Mori, Joanne Y. Yew, and Jacqueline S. R. Chin
- Subjects
Male ,laser desorption ionzation ,Biochemistry ,Pheromones ,Courtship ,Sexual Behavior, Animal ,D. mojavensis ,D. arizonae ,Biology (General) ,Mating ,Structural motif ,Phylogeny ,ozone-induced dissociation ,mass spectrometry ,media_common ,Sex Characteristics ,Ecology ,D. melanogaster ,Molecular Structure ,biology ,Reproduction ,General Neuroscience ,General Medicine ,Biological Evolution ,Drosophila melanogaster ,Crotonates ,Sex pheromone ,Medicine ,Female ,Desert Climate ,Research Article ,QH301-705.5 ,Science ,media_common.quotation_subject ,General Biochemistry, Genetics and Molecular Biology ,Hemiterpenes ,Phylogenetics ,Botany ,Animals ,Drosophila ,Triglycerides ,General Immunology and Microbiology ,behavior ,fungi ,biology.organism_classification ,Evolutionary biology ,Function (biology) - Abstract
Pheromones play an important role in the behavior, ecology, and evolution of many organisms. The structure of many insect pheromones typically consists of a hydrocarbon backbone, occasionally modified with various functional oxygen groups. Here we show that sex-specific triacylclyerides (TAGs) are broadly conserved across the subgenus Drosophila in 11 species and represent a novel class of pheromones that has been largely overlooked. In desert-adapted drosophilids, 13 different TAGs are secreted exclusively by males from the ejaculatory bulb, transferred to females during mating, and function synergistically to inhibit courtship from other males. Sex-specific TAGs are comprised of at least one short branched tiglic acid and a long linear fatty acyl component, an unusual structural motif that has not been reported before in other natural products. The diversification of chemical cues used by desert-adapted Drosophila as pheromones may be related to their specialized diet of fermenting cacti. DOI: http://dx.doi.org/10.7554/eLife.01751.001, eLife digest For animals, the ultimate purpose of life is to have sex, as nothing is more important than passing down your genes to future generations. A wide range of strategies are therefore employed throughout nature to maximize the chances of sexual success, from ostentatious courtship rituals to the subtle subliminal signals sent out using chemicals called pheromones. Plants and animals release pheromones to influence the behavior of other plants and animals, often without the recipient being aware of it. Hundreds of different insect pheromones have been discovered. Fruit flies release a number of different pheromones, all with similar chemical structures. Now, Chin et al. have discovered that male flies belonging to several species of fruit fly that live in the desert release chemicals called triacylglycerides (TAGs), which are commonly used for energy storage by many organisms as pheromones. During sex, the male fly rubs the TAGs onto the body of the female, which makes her less attractive to other male flies for several hours, thus increasing his chances of parenthood and passing his genes to future generations. TAGs are also found in other insect species, but have been largely overlooked as pheromones. Moreover, the TAGs discovered by Chin et al. have an unusual structure, not previously seen in nature, which may result from the diet of fermenting cacti the desert-dwelling fruit flies enjoy. DOI: http://dx.doi.org/10.7554/eLife.01751.002
- Published
- 2014
4. Author response: Sex-specific triacylglycerides are widely conserved in Drosophila and mediate mating behavior
- Author
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Qi Ling Koh, Jacqueline S. R. Chin, William J. Etges, Kenji Mori, Huong T. Pham, Stephen J. Blanksby, Joanne Y. Yew, and Shane R. Ellis
- Subjects
Evolutionary biology ,Mating ,Biology ,Drosophila (subgenus) ,biology.organism_classification ,Sex specific - Published
- 2014
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