Search

The current investigation was carried out for 100 random sample of meat products represented by frozen beef burger, frozen kofta, minced meat and oriental sausage (25 each) gathered from various shops and retail stores in Menofya governorate. The gathered samples were subjected to bacteriological examination for the isolation and identification of non O157 E.coli and E.coli O157 by using Conventional and recent techniques as multiplex PCR. By the conventional method the incidence of E.coli in the tested samples of beef burger, kofta, minced meat and oriental sausage was 6(24%), 9(36%) , 5(20%) and 11(44%) respectively. Multiplex-PCR technique was applied on 10 random meat product samples (3 negative and 7 positive for the isolation of E.coli by conventional method). M-PCR technique was applied in order to detect stx1 and stx2. In this study ,the M-PCR give negative result with all tested food samples. This study clarified that multiplex PCR may give negative results due to inhibitors that can be found in microbial DNA solutions extracted from meat sample or due to inhibitors which added during processing of meat products .

Shiga toxin producing Escherichia coli is a contaminant of food and water that causes a diarrheal syndrome followed by more severe disease of the kidneys and symptoms of the central nervous system in humans. The isolation of Shiga toxin producing Escherichia coli (STEC) from diarrheic and apparently health calves is difficult due to lack of differential phenotypic characteristics from nonpathogenic Escherichia coli. The improvement of molecular technology allows identification of both toxin and serogroup specific genetic determinants. In this study, 300 fecal samples from diarrheic and apparently healthy calves were screened for STEC using PCR targeting Shiga toxin determinants. In addition routine culture methods for isolating O157 and non O157 STEC were also performed. The screening assays of serotyping isolates revealed 7 (4.1%) of O157H7, 156 (92.8%) of non O157 and 5 (3.1%) for untypable strains. These included STEC serotypes of O157H7 and O26 from diarrheic samples, and O78, O55 and O126 from apparently healthy calves. The high rate of STEC isolation and the diversity of STEC serogroup from calves focus the light on the importance of calves as reservoir of E. coli as well as motivate us to improve biosecurity measures in dairy farms.

Lactoferrin has amajor effects on enteropathogenes as it inhibits growth. so, This work adopted to study the antibacterial activity of lactoferrin on growth of E. coli non O157 in broth and pasteurized milk. two strains of E. coli ( O1 carry stx2 and hly gene and O26 carry hly gene) were used. Different concentrations of lactoferrin (zero, 0.5, 1.0, 5.0, 10 and 20 mg/ml) were used in Lauria broth. Based on our results, LF showed various inhibition activity on E. coli non O157 growth in Lauria broth. Significant decrease observed on growth of E. coli O1. Higher concentrations of lactoferrin caused a significant decrease in E. coli O26 growth. Regarding its effect in pasteurized milk, a significant decrease in the count of E. coli O1and E. coli O26 at the concentrations of 10 and 20 mg/ml lactoferrin was noticed. So, lactoferrin could become a promising method to decrease growth of E. coli non O157 in pasteurized milk consequently decrease E.coli non O157 associated illness in humans.

The objectives of the current study were detection and characterization of the isolated Escherichia coli non O157 using PCR assay. A total of 90 cheese samples high salt soft cheese, Kareish cheese and Tallaga cheese were examined for E. coli non O157 using modified vancomycin- trypticase soy broth and Sorbitol MacConkey agar plates. Serodiagnosis of E. coli non-O157 has been done using slide agglutination test. Toxigenic E. coli non-O157 isolates were detected using PCR assay. Results postulated that the detection rate of non O157 using biochemical technique was 63.33% in Kariesh cheese, 20% in high salt soft cheese cheese, and 33.33% in Tallaga cheese. Ten different serotypes of E. coli non O157 have been distributed as following O1 (4.44%), O18 (1.11%), O20 (1.11%), O25 (1.11%), O26 (3.33%), O125 (1.11%), O126 (1.11%), O127 (1.11%) and untyped E. coli (18.89%). Regarding PCR results, serotypes group (O1) was positive for Stx2 gene, (O1 and O20) were positive for both Stx2 and hly genes, one serotype group O25 was positive for eaeA gene, one serotype group O26 was positive for hly gene while no detection for Stx1and STa genes. From the aforementioned data, attention must be paid to the problems of E. coli non O157 in foods. Consequently, more restriction and preventive measures should be taken in milk herds, milk production and dairy factories in respect to quality control sanitation and health care.