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Blood typing is a critical test in many medical procedures, especially for blood transfusion. Incompatible red blood cells (RBCs) in transfusion might result in intravascular hemolysis and even cause recipients death. The test result of blood typing can be determined by observing the agglutination of RBCs. In this study, a microfluidic blood typing chip with interdigitated electrodes is presented. ZnO nanowires were synthesized on the interdigitated electrodes so that voltammetry measurement was employed to determine the test results of blood typing. The experimental results show that the discrimination between agglutination and non-agglutination was obvious and successful determinations of RBC agglutinations in blood typing tests were achieved by means of voltammetry measurement.