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The behavior of glycolysis concerning the biochemical reaction was thought to be similar to the oscillatory reaction with glucose being the substrate. The objective of this research is to find a new oscillatory system and study its relevant properties, so new Cu (II)-catalyzed oscillating reaction involved glucose and acetone as the double substrates was investigated systematically. As a function of reaction temperature and concentration of glucose, bromate, and acetone, the features in this novel oscillation system was studied. The results showed that the activation energy reached up to 90.839 KJ/mol and the oxidized product of the double substrates provided the impetus in the course of the new oscillating reaction. A tentative mechanism was considered on the basis of FNK.