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Crayfish utilize aquatic plants as a food source and may concentrate pollutants, such as heavy metals. Monitoring the possible heavy metal presence in crayfish edible tissues is therefore important since crayfish can be part of the human diet. In this study we have analysed specimens of Cherax destructor of two different sizes coming from a crayfish farm, with respect to heavy metal (lead, copper, cadmium, chromium, zinc) concentrations in the muscle, hepatopancreas and exoskeleton. Furthermore, the metal concentrations were determined in water, sediment and in plants. Our data show that the contents of lead, copper, cadmium, chromium and zinc, are higher in the tissues with respect to water, indicating in some cases their bioaccumulation in Cherax destructor. The content of lead and zinc in sediment and plants the animals feed on, was higher than in tissues and organs, suggesting that biomagnification does not occur for these heavy metals. Copper and chromium show biomagnification in all tissues, while cadmium only in the hepatopancreas.