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In this study, the effect of process parameters (incubation period, temperature, initial pH-value, concentration of PAHs in the medium, and bacterial inoculum size) on the biodegradation of PAHs using Mycobacteria confluentis was studied using One-Factor-At-a-Time (OFAT) method. From the results of the study, it was observed that the studied parameters had significant effects on the degradation process. The capability of Mycobacteria confluentis on the degradation of PAHs was found to be maximum when the initial pH of the PAH was 7, temperature 40 °, PAH concentration of 50 μL, bacterial concentration of 7.5 mL, and incubated for 3 to 5 days. This condition gave a PAH degradation percentage of 40 to 50 %, showing a similarity to most previous studies where the maximum percentage of degradation has been around 40 to 50 %. This study, therefore, concludes that Mycobacteria confluentis is a good PAHs degrader which can be of significant important in the management of oil polluted fields and environments.

Dental caries considered as one of the most common infectious diseases affecting mankind today, the dissolution of tooth structure by acid produced as a result of the fermentation of dietary carbohydrates by oral bacteria. The antimicrobial susceptibility of medicinal plants including black pepper, black cumin, cinnamon, cardamom on toothpaste and mouthwash against oral isolates for healthcare improvement was studied. Different oral isolates from 50 selected individuals aged 3 to 60 years were obtained from both genders, and a total of 59 bacterial and yeast isolates were collected, purified, and tested against four different commercial medicinal plants extracts for antimicrobial susceptibility profile. A total of 10 mouthwashes was purchased, and the combined action of the medicinal plants with mouthwash was studied against oral isolates. We found a higher bacterial population was in the age group of 3–40 years than other two groups, with approximately 44%. In addition, the combined action of acetone plant extracts (alone) against oral isolates showed increasing effect profile up to 61.02% when combination A (Ci/N) was added. Also, While, the combined action of medicinal plants with mouthwash was improved 100% with combination C. We conclude that the combination C (P/N/Ca/Ci) with mouthwash showed high susceptibility against oral isolates.