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This paper shows the results of the study of the effect of temperature on the coagulation processes in the hemostasis of cows and calves. Purpose of the work: comparison of the results of the study of the indicators of the coagulation system of blood of cows and calves of the black-and-brown breed, obtained with the use of medical and veterinary coagulometer, taking into account the age of the animal and its body temperature. The study was conducted in the Vologda region of Russia. The following coagulogram parameters were analyzed: thrombin time, prothrombin time, activated partial thromboplastin time, fibrinogen count. It was found that at physiological temperature on a veterinary coagulometer in the plasma of cows there is a reliable increase in thrombin time by 2.2 times, decrease in prothrombin time by 32.5%, increase in activated partial thromboplastin time by 11.2%, increase in the amount of fibrinogen by 315.4%; in calf plasma - decrease of thrombin time by 7.8%, increase of prothrombin time by 6.2%, increase of activated partial thromboplastin time by 20.9%, increase of fibrinogen amount by 347.6% in comparison with the results obtained during standard incubation at 37°C. The correlation between hemostasis indicators and temperature factor in cows was noticeable (thrombin time, fibrinogen), in calves negative moderate for thromboplastin time, moderate for activated partial thromboplastin time and noticeable for prothrombin time and fibrinogen. The study of coagulation processes in cows and calves using a medical coagulometer led to the results that differ significantly from the reactions occurring in the organism at physiological body temperature. Taking into account the results of the study, it is possible to consider a veterinary coagulometer with the possibility of setting the necessary temperature as a device quite suitable for adequate assessment of hemostasiological parameters in cattle.