1. Brachyspira (Serpulina) pilosicoli of human origin interfere with the haemolytic activity and the growth of Staphylococcus aureus beta-toxin producer.
- Author
-
Calderaro A, Dettori G, Ragni P, Guegan R, Arcangeletti MC, Medici MC, and Chezzi C
- Subjects
- Bacterial Toxins antagonists & inhibitors, Humans, Microbial Sensitivity Tests, Spirochaetales growth & development, Staphylococcus aureus growth & development, Antibiosis physiology, Bacterial Toxins biosynthesis, Hemolysis, Spirochaetales physiology, Staphylococcus aureus physiology
- Abstract
Brachyspira (Serpulina) pilosicoli related to intestinal spirochaetosis were found to interfere in vitro with the haemolytic activity and the growth of Staphylococcus aureus beta-toxin producer. This interference was clearly appreciated because a reduction of the zone of the staphylococcal beta-toxin activity, the reduction and/or absence of cooperative haemolysis between bacteria, and the growth reduction of S. aureus were observed when B. (S.) pilosicoli were grown 72-96 hours sooner than S. aureus and after the inoculum of the latter the plates were anaerobically incubated for additional 48-72 hours. The phenomenon was more clearly observed when B. (S.) pilosicoli had a concentration of 8x10(6)-8x10(7) CFU/ml and S. aureus at a concentration ranging from 10(7) to 10(1) CFU/ml was inoculated at a distance from the streaks of B. (S.) pilosicoli ranging from 0-10 mm. When B. (S.) pilosicoli and S. aureus were inoculated at the same time and when B. (S.) pilosicoli grew 24-48 hours sooner than S. aureus only a cooperative haemolysis was observed.
- Published
- 2002