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[Objective] The present paper was conducted to popularize the natural herbage among vineyards in the eastern foothill of Helan Mountain. [Method] In this experiment, natural herbage for 1 year and cleared vineyard were taken as contrast. The effects of natural herbage on soil nutrients, enzyme activity and microorganism in different soil layers of vineyard were studied in Yunhao winery of Lanshan, Jins-han, eastern foothill of Helan Mountain. [Result] The soil alkali-hydrolyzed nitrogen and available phosphorus content were higher than that of the control, however, the pH, total salt, total nitrogen, total phosphorus, available potassium and organic matter content decreased, the number of bacteria, fungi and actinomycetes in different soil layers of natural herbage for 1 year vineyard soil increased,and the deep soil layer (40 -60 cm) was more obvious than the surface soil layer (0-20 cm), and natural herbage for 1 year could improve urease, sucrase and catalase in vineyard, especially for the surface soil layer (0 -20 cm), and the increase of soil enzyme activity was more obvious. [Conclusion]The natural herbage in vineyard can improve the soil condition to some extent, reducing soil pH, decreasing soil salt content, increasing soil microorganism and improving soil enzyme activity that provides a comfortable growth environment for grape growth, and then improves grape and wine quality, which is a good management method for vineyard soil in the eastern foothill of Helan mountain. [ABSTRACT FROM AUTHOR]