Search

[Objective] In order to provide theoretical basis for pollen viability identification, artificial pollination and cross breeding of large- fruited yellow passion fruit, the effects of different culture medium in vitro and storage conditions on pollen germination were analyzed. [ Method] Pollens were collected from newly blooming flowers of large-fruited yellow passion fruit The suitable concentrations of sucrose, boric acid, calcium nitrate and suitable pH for pollen in vitro culture were screened by Li, (5 4) onhogonal design. The effects of sampling time of flowers, storage humidity and temperature on pollen viability were studied by using the most suitable pollen culture medium. The suitable storage condition for pollen of large-fruited yellow passion fruit was determined. [Result] The results of onhogonal test showed that the order of influencing factors on pollen germination rate of large-fruited yellow passion fruit was calcium nitrate > sucrose > boric acid > pH. The optimum medium for pollen germination in vitro was 100. 00 g/L sucrose + 0. 02 g/L boric acid + 0. 40 g/L calcium nitrate, and the medium pH was 5. 0. The pollen germination rate in the optimum medium was 85. 55 % . The pollen germination rate of large-fruited yellow passion fruit was more than 80. 00% when the sampling time was from 11 :30 to 17 :30. The pollen viability was the highest in the third hour ( 16: 30) after flowering, which was significantly higher than the germination rate of pol- lens collected at other times except 13 : 30 and 15 : 30 (P <0. 05, the same as below). The results of storage test showed that, the pollen germination rate of natural air drying on sulfur paper treatment for 3 - 48 hours was significantly higher than that of sealed centrifugation tube storage at room temperature and storage in desiccator on sulfur paper for the same time, and the pollen germination rate of 48 h storage was still 34. 28%. The pollen germination rate was 61. 49% after natural air drying for 5 hours and sealed in centrifugal tube at 4 °C for 24 hours, which was significantly higher than that (55. 22%) after natural air drying for 5 hours and sealed in centrifuge tube at - 20 °C for 24 hours. [Conclusion] The optimum medium for pollen germination in vitro of large-fruited yellow passion fruit is 100. 00 g/L sucrose+ 0. 02 g/L boric acid+ 0. 40 g/L calcium nitrate, and pH is 5. 0. The best storage condition is that the pollens collected on the day are dried naturally for 5 hours and sealed in centrifuge tube at 4 °C [ABSTRACT FROM AUTHOR]

【Objective】stem disinfection, bud induction and proliferation were explored during tissue culture to provide technical support for mass seedling production.【Method】 Taken stem of Hylocereus undatus cultivar Guihonglong No. 1 as the explants, the effects of different factors on stem disinfection, bud inducement and proliferation were studied.【Result】 Stem size was the main factor affecting the pollution rate of stem disinfection, followed by the effect of sterilization time and stem mature degrees. The sterilization time had the greatest influence on the bud induction, followed by the 6-BA concentration. Both the stem mature degrees and stem size had little effect on bud induction. 6-BA 4.0 mg/L had the best induction effect. The higher concentration of 6-BA showed the higher multiplication ratio of buds, but when 6-BA concentration reached 4.0 mg/L, the buds appeared vitrification. The effect of IAA was better than that of NAA, and the effect of bud proliferation was better when IAA was 0.1 mg/L. PP333 was better than AC in inhibiting vitrification and promoted subculture multiplication of Hylocereus undatus. 【Conclusion】 The optimal treatment for stem disinfection and bud induction was suggested as: sliced the health stems of about 1 months into pieces with single bud, and disinfected them with 0.1 % HgCl2 solution, and inoculated the explants in medium of MS + 6-BA 4.0 mg/L. The optimal medium for bud proliferation was MS +6-BA 3.0 mg/L + IAA 0.1 mg/L + PP333 1.0 mg/L. [ABSTRACT FROM AUTHOR]