Search

[Objective] The present paper aimed at determining the occurrence of chilli veinal mottle virus (ChiVMV) in tobacco in Guizhou and the genetic evolution relationship between the ChiVMV strains in Guizhou and in other provinces in China, so as to provide a basis for formulating scientific and reasonable prevention and control measures. [Method] 80 tobacco leaf samples suspected of virus infection were collected from the tobacco fields of Guiyang, Zunyi, Anshun and Tongren in Guizhou province, showing the symptoms of leaf mottling, wrinkling, necrosis and mosaic. Multiple viral primers were designed to detect the collected samples by RT-PCR method, and the detected ChiVMV was further amplified by PCR to obtain the whole genome sequence of Guizhou tobacco ChiVMV. Based on all ChiVMV genome sequences published by NCBI, sequence consistency analysis was performed and phylogenetic evolutionary tree was constructed to study the genetic evolutionary relationship of Guizhou ChiVMV tobacco isolates. [Result] The results of RT-PCR showed that the detection rate of ChiVMV was 37. 50%, the detection rate of tobacco mosaic virus (TMV) was 48. 75%, and the detection rate of potato virus Y (PVY) was 35. 00%. The detection rate of cucumber mosaic virus (CMV) was only 8. 75%. Four complex infection types TMV + PVY, PVY + ChiVMV, PVY + CMV and ChiVMV + PVY + TMV were detected, and the detection rates were 26. 25%, 6. 25%, 10. 00% and 3. 75%, respectively. Guizhou tobacco isolate ChiVMV-GZ (GenBank: OP589298) was obtained by PCR. Sequence consistency analysis showed that ChivMV-GZ was identical with tomato isolates from Sichuan (KC711055) and Sichuan tobacco isolates (MK405594) with high sequence consistency (98. 70% and 98. 50%). Phylogenetic tree was constructed and it found that ChivMV-GZ clustered with ChiVMV strains from Sichuan and Yunnan. [Conclusion] The damage of ChiVMV in Guizhou tobacco has been increasingly serious and sufficient attention should be paid. ChiVMV-GZ was isolated from Guizhou tobacco and amplified to obtain the whole genome of the virus in the study. Based on genetic evolution analysis, it was found that ChiVMV-GZ was most closely related to ChiVMV strains from Sichuan and Yunnan. [ABSTRACT FROM AUTHOR]

[ Purpose] The present paper aimed to study the physical properties, soil enzyme activities and their correlation characteristics of wild Bletilla striata soil in different producing areas in Guizhou, so as to provide a theoretical basis for realizing high yield and quality of B. striata artificial cultivation. [ Method] 8 soil sample plots for the wild B. striata were collected in Guizhou province to detect and analyze the soil physical and enzyme activity index characteristics and the correlation between them. [ Result]The soil density was between 2. 06 -2. 55 g/ cm³ with weak variation. The soil texture was mainly clay loam and sandy loam. In most soils, the proportion of the > 0. 25 mm size soil water-stable aggregates reached 70% . The distribution of the soil water-stable aggregates size fraction were different, 2 - 5 mm size fraction was the dominant size fraction in 4 soil plots, < 0. 25 mm size fraction was the dominant size fraction in 3 soil plots. The dominant size fraction of soil micro-aggregate was 0. 25 -0. 05 mm size fraction. The activities of urease, acid phosphatase and sucrase in soil were significantly different with strong variation. The activities of catalase in soil were not significantly different with weak variation. Soil enzyme activity was strongly influenced by the soil density, soil aggregates and microaggregates. There was a significant positive correlation between urease and acid phosphatase, which could promote each other. [Conclusion] Guizhou wild B. striata has strong adaptability to soil environment. It mainly grows in sandy soil or clay with soil clay content less than 25 %, and some wild B. striata can also grow in clay with soil clay content more than 30% ; Soil specific gravity, soil aggregates and micro aggregates are important factors affecting soil enzyme activity of B. striata. The suitable soil specific gravity is 2. 06 - 2. 55 g/ cm³, the dominant particle size is 2 - 5 mm or < 0. 25 mm, and loam is the best soil texture; Urease and acid phosphatase can promote each other. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to verify pathogenic bacteria of kiwifruit canker and provide effective strain resources for biological control against kiwifruit canker.【Method】The pathogenic bacteria were isolated from infected issues of kiwifruit plants infected with canker by a convention tissue isolation method. The antagonistic strains were screened from the bacteria isolated from soil and healthy kiwifruit tissues by an inhibition zone method. The isolated antagonistic strains were identified by morphology and molecular biology methods. 【Result】9 strains isolated from the infected tissues of kiwifruit plants infected with canker were identified as Pseudomonas syringae pv. Actinidae. 35 strains were isolated from soil and healthy kiwifruit tissues. 3 screened Bacillus strains had the good antagonism effect against Pseudomonas syringae pv. Actinidae and the average diameter of inhibitory zone was 20.8-23.7 mm.【Conclusion】Three screened Bacillus strains lay a foundation for development and application of biological agents against kiwifruit canker. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to provide the scientific basis for rational land-use planning, soil resources conservation and pollution-free agricultural development in Luodian County, Guizhou. [Method] The effects of five typical land-use patterns (forestland, natural grassland, artificial grassland, cropland and abandoned farmland) on content and environment contamination of Cd, Cr, Pb, Ni, Hg and As in topsoil were evaluated by Nemerow comprehensive index, single-factor contamination index and Hakanson potential ecological hazard methods. [Result] The content of 6 heavy metals in topsoil was Cr > Pb > Ni > As > Cd > Hg and lower than the contamination background value of heavy metals in Guizhou overall. The topsoil Cd content was the highest in abandoned farmland and lowest in forestland. The topsoil Cr content was the highest in abandoned farmland and lowest in natural grassland. The topsoil Pb and Hg content was the highest in cropland and lowest in forestland. The topsoil Ni content was the highest in artificial grassland and lowest in natural grassland. The topsoil As content was the highest in natural grassland and lowest in cropland. The topsoil Cd, C.r, Pb and Hg content was high relatively and greatly influenced by human activity. Topsoil Cr had the same source as Ni, Hg and As. The topsoil As content was closely related to soil pH. The topsoil Cd and Cr content was closely related to soil cation exchange capacity (CEC) . The topsoil Pb, Hg and As content was closely related to soil organic matter (SOM). [Conclusion] Six heavy metals in topsoil do not cause soil contamination. The single-factor contamination index and potential ecological hazard index both are at the clean level. The pollution-free agriculture can be vigorously developed according to local soil nutrient, soil fertility and land-use pattern in Luodian County, Guizhou. [ABSTRACT FROM AUTHOR]

[Objective] This paper aims to provide theoretical and technical support for the efficient pesticide application of spring potato in high altitude areas of Guizhou. [Method] A field experiment was conducted on Weiyu 5, to explore the effects of different pesticide application dosage on disease index and biological effect of spring potato in Guizhou. [Result] After spraying the potato seedling rate were 89. 63% — 93. 37%, the disease index were 2.71% — 6. 17%. Compared with CK, the yield, number of single tuber and the yield of single plant increased by 1.55% —22.97%,2. 50% —30.00%,and 12.29% —42.68%,respectively. The best performance was obtained with 80% of the customary spraying dose. Pesticide spraying can significantly improve reducing sugar and starch content of potato, increase the reducing sugar content by 0. 01 —0. 14 percentage and starch content by 0.76 —7.43 percentage compared with CK. But the nitrogen, phosphorus, potassium and moisture content were not obviously affected. In terms of soil nutrient changes, the contents of soil organic matter, available potassium, available phosphorus, and alkalize nitrogen were increased by 6. 96% — 15. 47%, 1 . 75 — 36. 84%, 3. 57% —56. 09% and 10. 00% —14.99% respectively compared with CK. [Conclusion] In summary, 80% of customary spraying dose is recommended for high-yielding dryland spring potato cultivation in Guizhou. [ABSTRACT FROM AUTHOR]

[Objective] The objective of the paper is to explore a rapid and accurate method for diagnosing the biochemical components of Guizhou premium fruit plant. [ Method] Taking hyperspectral data of Passiflora edulis leaves and its corresponding leaf chlorophyll content as the data source, mathematical transform and discrete wavelet transform algorithm were used to process and analyze the spectrum data of P.edulis leaves, the sensitive bands were extracted by correlation analysis, and then the estimating model of chlorophyll content in P.edulis leaves was established by using partial least square algorithm. [ Result] The low frequency information of logarithmic transform separated from discrete wavelet transform and mathematical transform could significantly improve the estimation of spectrum to chlorophyll content in P. edulis leaves. The model established based on discrete wavelet transform L1 scale was optimum, which had high estimated accuracy and stability with R² = 0. 578 and RMSE = 1. 104. [Conclusion] As affected by complex geographical environment and climate condition, the spectrum data of P. edulis leaves contains a large amount of noise. The high frequency information extracted by mathematical transform and discrete wavelet transform has weak abilitying estimating chlorophyll content in P. edulis leaves. Compared with high frequency information, low frequency information has strong estimating ability of chlorophyll content in P. edulis leaves. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to reveal the landscape pattern evolution and driving factors of karst rocky desertification area, and provide a reference for optimizing the land landscape pattern, rocky desertification control and regional sustainable development in the demonstration area. [Method] Taking the typical Karst Plateau Bijie Salaxi demonstration area as the research object, the remote sensing images of three key time periods were selected, including ETM in 2005, 2010 and OLI in 2015. 3 S technology and landscape index analysis method were employed to study landscape pattern and the driving factors. [Result] The main landscape of the study area was cultivated land, followed by shrub forest. The changes of landscape transfer were mainly manifested in the transformation from cultivated land to forest, grassland and construction land, which accounted for 73. 31 % and 24. 75 % of the cultivated land transfer respectively in 2010; By 2015, they accounted for 79. 80 % and 19. 99 % respectively. At the landscape type level, the fragmentation degree of cultivated land, forestland and shrub land was increasing, and the fragmentation degree of grassland and construction land increased first and then decreased. Except for the unused land, the landscape patch shape tended to be complicated. In the overall level of the landscape, the landscape of demonstration area had developed in the direction of fragmentation, complexity, discretization and homogenization in the last 10 years. [Conclusion] The implementation of rocky desertification control project, urbanization construction and land abandonment were the main factors leading to the change of landscape pattern. [ABSTRACT FROM AUTHOR]

【Objective】The objective of this paper was to understand the content and enrichment characteristics of trace elements in soil and plant(root) of wild Berberis in Guizhou Province and to provide the theoretical reference for cultivation of wild Berberis. 【Method】The contents of trace elements in soil and plant(root) were determined, and the correlation of trace elements between soil and plant(root) and the enrichment ability of plant(root) to trace elements were discussed. 【Result】Total amount of soil trace elements of Berberis guizhouensis Ying, B. pruinosa var. barresiana Ahrendt, B. weiningensis Ying, B. tsienii Ying and B. deinacantha Schneid. was Fe > Mn > Zn > Cu > Mo; B. veitchii Schneid., B. wilsonae var. guhtzunica(Ahrendt) Ahrendt, B.pingbaensis M.T.An was Fe>Mn>Cu>Zn>Mo. The highest content of the soil total amount of Cu was B. veitchii, the total amount of Fe was B. tsienii, the total amount of Mn was B. weiningensis, the total amount of Mo was B. deinacantha, the total amount of Zn was B. weiningensis. The highest content of the soil available amount of Cu was B. guizhouensis, the available amount of Fe was B. veitchii, the available amount of Mn and Mo was B. weiningensis, the available amount of Zn was B.wilsonae var.guhtzunica.The highest content of Cu and Mn in the roots of was B.wilsonae var.guhtzunica., Fe was B. deinacantha, Mo was B. deinacantha, Zn was B. tsienii. 【Conclusion】The trace elements between soil and plant(root) were correlated to some extent. The enrichment coefficient of Cu of B. guizhouensis was the highest and was greater than 1, and the other trace elements enrichment coefficient was less than 1.The content of the available trace elements in the soil of non-area of Berberis in Guizhou Province was more obvious than that of the whole area. [ABSTRACT FROM AUTHOR]

[Objective] The objective of the paper is to provide the scientific basis for rational fertilization in Jianhe County of Guizhou Province and similar areas. [Method] The effects of soil nutrient indexes(total N .organic matter, available N .available K, available P, total P and total K)on soil fertility are explored by using variation coefficient method, correlation coefficient method and weighted mean method, and then the soil fertility in paddy field and dry' land is evaluated by using improved Nemerow comprehensive evaluation method combined with the weight coefficient mean value of the three methods. [Result] The soil available k in paddy field and total P in dry land were at a lower level, while other nutrient indexes were at a medium-high, high and higher level. The soil available N content in six dry' land sampling sites with different plants was high. Soil total P and available k fluctuate greatly among sampling sites, while organic matter content fluctuates less. The soil available P in fertility factor weighted coefficient of paddy field and dry land was the highest, respectively 0. 2710 and 0. 2358 comparing the mean value of the three weighted method weighted coefficient. The available P is the main factor influencing soil fertility in paddy field and dry' land and soil fertility indexes is 1.52 and 1.63 separately. The soil comprehensive fertility was at a fertile level. [Conclusion] The soil fertility of paddy field and dry land in Jianhe County is at a fertile level, and available P is the main factor influencing soil fertility in paddy field and dry land. The soil N content in paddy field and dry land is high, which can meet the demand of crop production. Therefore, N fertilization should be reduced in production. The soil K content in paddy field is low, and the corresponding fertilization measures should be taken to improve K supply level in paddy field. [ABSTRACT FROM AUTHOR]

[Objective]The aim of this paper was to provide a reference for edible rose cultivation and industrial development in Guizhou, and to know the adaptation of the four edible roses, Rosa glauea(RG), Rosa damascene(RD), Pingyin rose(RP) and White rose(RW), to the climatic environment in Guiyang, Guizhou, through the quality analysis of color of flowers, shape of petals, weight of flowers, characteristic of leaves, and composition of essential oils. [Method]Biological traits were obtained by field invesligation and statistics;the essential oils were extracted with steam distillation, and analyzed by GC-MS. [Result] 39 aroma components were observed in RG, RD, RP and RW19, 22, 10, and 26 kinds of components were respectively detected from RG, RD, RP, and RW, accounting for 99. 48 %, 98. 00 %, 94. 00 %, and 100. 00 % in total aroma components. Flavoring composition in RG were citronellol and geraniol(51. 44 % and 20. 47 %), and that in RD were geraniol and nerol(32. 72 % and 22. 71 %), in RP were citronellal and geraniol(55. 87 % and 24. 59 %), and in RW were also citronellal and geraniol(28. 21 % and 30. 38 %). Essential oils component in RD and RW were the closest to ISO 9842:2003. Linalool, terpineol, geraniol, acacia alcohol and n-heptadecane were observed in volatile oils of RG, RD, RP, and RW, accounting for respectively 24. 78 %, 46. 28 %, 30. 83 %, and 37. 54%. [Conclusion] RD, RW and RG well adapted to the climatic environment of Guiyang area, and could be cultivated and generalized. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to understand chemical properties and available microelement characteristics of soil for growth of wild Bletilla striaes, explicit suitable soil environment of Bletilla.striaes cultivation and provide a reference for soil environment selection of Bletilla.striaes artificial cultivation. [Method] The microelement effective state and chemical properties of soil for growth of wild Bletilla.striata were investigated by field survey and the sufficient and lack level was analyzed by indoor analysis to discuss restriction factors affecting soil available microelement content. [Result] The pH and content of organic matter, total nitrogen, total phosphorus, total potassium, available nitrogen, available phosphorus, rapid available potassium, available Cu, available Zn, available Fe and available Mn in soil of Bletilla.striata cultivation are 7.65-7.99, 26.86-280.58 g/kg, 1.55-14.44 g/kg, 0.47-0.99 g/kg, 1.89-19.52 g/kg, 99-641 mg/kg, 2.40-18.75 mg/kg, 70-122 mg/kg, 0.45-1.70 mg/kg, 0.83-14.66 mg/kg, 11.80-119.85 mg/kg and 24.88-52.55 mg/kg respectively. [Conclusion] The suitable soil environment for Bletilla.striats growth is alkalescence and needs higher content of organic matter, total nitrogen, total phosphorus, available nitrogen, available phosphorus and rapid available potassium. The soil organic matter, N, P and K content has no obvious effect on Cu and Mn content in soil. The content of organic matter, total nitrogen, available nitrogen and available phosphorus in soil is positively related to Zn and Fe content very significantly.Selecting the soil with higher content of organic matter, total nitrogen, available nitrogen and available phosphorus can improve available Zn and Fe content in soil during Bletilla.striata artificial cultivation process, which is beneficial to growth and quality of Bletilla striata. [ABSTRACT FROM AUTHOR]

[Objective]The aim of this paper was to explore the distribution characteristics of soil nutrients in different recovery modes of rocky desertification control, and to provide scientific basis for further optimizing the vegetation allocation of rocky desertification control. [Method] Taking soil from five sampling sites as the study object, including Zanthoxylum bungeanum, Lonicera japonica, mixed forests, cardamom and abandoned farmland in Huajiang Rocky Desertification Control Demonstration Area in Guizhou. Soil layers of 0 - 10, 10 -20 and 20 -30 cm were sampled and monitored for 3 years before, during and after rainy season, and the distribution characteristics of soil nutrients under different recovery modes were analyzed. [Result] With the restoration of vegetation, compared with abandoned farmland, average values of soil bulk density, soil retention ability and capillary porosity in the four plots decreased respectively by 0.61 %, 7.97 % and 1.12 % , soil organic carbon, total nitrogen, total phosphorus and total potassium increased by 53.45 %, 45.72 %, 80.81 % and 1.44 % respectively, hydrolyzed nitrogen and available phosphorus increased by 53.36 % and 25.80 % , respectively. In the same plot, the contents of soil organic matter and soil nutrient contents were all shown as during rainy season > after rainy season > before rainy season. With the increase of depth of the soil layer, content of soil nutrients of different recovery models decreased, nutrients mainly concentrated in the surface layer. [Conclusion] There were differences in nutrient contents between different restoration models, and vegetation restoration in karst rocky desertification area had an ameliorative effect on ecological environment. [ABSTRACT FROM AUTHOR]

【Objective】 The aim of this paper was to reveal the ecological risk of heavy metals in farmland soil in Liping County of Qiandongnan prefecture, and provide scientific basis for safe production of crops and prevention and control of heavy metal pollution in farmland soil.【Method】 The single factor pollution index, Nemero comprehensive pollution index and Hankanson potential ecological risk index method were used to analyze pollution degree of chromium(Cr), lead(Pb), cadmium(Cd), arsenic(As) and mercury(Hg) in cultivated soil in Liping County, and their potential ecological risks were evaluated as well.【Results】The average concentrations of Cr, Pb, Cd, As and Hg in the soils were 68.39, 23.55, 0.22, 6.16, 0.15 mg/kg, respectively. Hg concentration was 1.36 times as the background value of soil in Guizhou province. The average single factor pollution index of Cr, Pb, Cd, As and Hg were 0.74, 0.47, 0.31, 0.19 and 0.09, respectively. The over standard rates of Cd was 3.4 %. The mean value of Nemerow integrated pollution index was 0.68, indicating that there was no pollution in Liping county. The low, medium and heavier potential ecological risk levels of Cd accounted for 91.5 %, 6.8 % and 1.7 % respectively. The low and medium potential ecological risk levels of Hg accounted for 95.9 % and 4.1 % respectively. 【Conclusion】There is no potential ecological risk for heavy metals in cultivated soils in Liping county. However, there is still existence of ecological risk for relative heavy Cd level and moderate Hg level in some villages and towns. [ABSTRACT FROM AUTHOR]

[Objectives]The purpose of this paper is to explore the effect of water retention agent (SAP) on potato growth in high altitude area of Guizhou, and to provide references for high-yielding cultivation of potatoes and efficient application of water and fertilizer in Guizhou. [ Methods] A field experiment including water retention agent dosage is done. This research takes the ‘ Taro 5 ’ as the experimental material, five water retention agent dosages(0, 37.5, 52.5, 67.5, and 82.5 kg/hm2 ) is arranged to study the effect of SPA on the yield, potato character, nutrient quality and soil fertility of potato, to determine the appropriate amount of water retention agent in this area. [ Results] The results showed that the potato seedling rate after applying the water retention agent was up to 93. 37 % , the yield increased by 7.96 % - 13. 37 % compared with T1 treatment, and the yield of single plant was 6.81 % -45.45 % , and the yield of single plant increased by 34. 92 % -83.31 %. Application of super absorbent polymers could significantly improve the potato starch content by 2.49 -5.35 percentage compared with T1 treatment, but the nitrogen, phosphorus, potassium, moisture content and reducing sugar content had no obvious effect. In terms of soil nutrient changes, the contents of soil organic matter, total nitrogen, total phosphorus, available phosphorus and available potassium were increased by 4. 61 % -27.01 % , 0 -19.01 % , 7.83 % - 15. 65 % , 5. 73 % -22.49 % and 5.08 % -25.42 % respectively compared with T1 treatment. [ Conclusions] The recommended application of water-retaining agent 52.5 -82.5 kg/hm2 was beneficial to high yield and soil fertility of potato in the high altitude area of Guizhou. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to explore the structure and spatial distribution pattern of ecological-living-productive land with different function at village level and provide the decision basis for optimization of spatial structure and function of national land in Yinjiang County.【Method】 The diversity, dominance, combination number and combination type of ecological-living-productive land were calculated by taking 351 villages as analysis scales in Yinjiang County, dividing land type according to ecological, productive and living function and using quantitative geography and GPS. The structure and spatial distribution of ecological-living-productive land were analyzed by the indexes of level and location degree.【Result】(i) The diversity index of ecological-living-productive land was 0.81 and the diversity index was at the above-average level overall. The dominant index was 0.58 and the dominance was at the below-average level overall.(ii) The combination types of ecological-living-productive land concentrated upon the ecological-productive ecological double combination type, productive ecological-ecological double combination type and single ecological type.(iii) The utilization degree of ecological-living-productive land was 231 with a higher overall level.(iv) The location difference among types of ecological-living-productive land at village level was obvious. The ecological land in east and south region was of obvious location advantage. The productive-ecological land in north, northwest and central region was of obvious location advantage. The living ecological land in west region was of obvious location advantage.【Conclusion】The structure and spatial distribution of the lands with different function can be calculated by the quantitative geographic model, diversity index, dominance, combination number and type, utilization level and location degree in land utilization classification of ecological-living-productive land. [ABSTRACT FROM AUTHOR]

【Objective】The present paper was conducted to definite the characteristics of weed community in kiwifruit orchards and provide the basis for prevention and control of weeds in kiwifruit orchards in Guizhou. 【Method】The weed communities in kiwifruit orchards in Shuicheng and Xiuwen county were investigated by a visual observation.【Result】100 weed species, 34 families were found in 11 sample plots and the species of Asteraceae, Gramineae, Polygonacaea and Lamiaceae were 26, 13, 9 and 5 respectively. The number of invasive alien weed species was 20 and 60 % species of total invasive alien weeds belonged to Asteraceae. The occurrence frequency of Digitaria sanguinalis. Galinsoga quadriradiata and Artemisia lavandulaefolia reached 90.91 %, 81.82 % and 72.73 % respectively. Five weed species with the maximum niche breadth value were Solanum nigrum, Digitaria sanguinalis, Crassocephalum crepidioides, Artemisia lavandulaefolia and Commelina communis. Some invasive alien weed species such as Solanum nigrum, Digitaria sanguinalis, Crassocephalum crepidioides, Artemisia lavandulaefolia and Commelina communis occured in kiwifruit orchards seriously. There are 24.73 weed species in each sample plot averagely. The average value of Shannon index, Pielou index, Jaccard differential index and Bray-Curtis differential index are 14.41, 0.84, 0.82 and 0.82, respectively.【Conclusion】The weed diversity of kiwifruit orchards is very high and the invasive alien weed species bring about serious challenges to prevention and control of weeds in kiwifruit orchards in Guizhou. [ABSTRACT FROM AUTHOR]

【Objective】 This paper aimed to reveal the heavy metal content and safety risk of river terraced rice field soil in mountain area. 【Method】 27 soil samples were collected from Jiming Village, Qingxi Township, Zhenyuan City of Guizhou Province. The contents of heavy metals(Cd, Cr, Pb, Zn, Cu) were determined, and then the contamination status and potential risk were evaluated by adopting index methods of single factor, comprehensive pollution, geoaccumulation and potential ecological risk. The source of heavy metal contamination in river terraced rice fields were estimated with the correlation analysis of heavy metals and principal component analysis. 【Result】 The average contents of Cd, Cr, Pb, Zn, Cu contained in the river terraced soil was 1.5, 61.51, 32.79, 160.78 and 29.61 mg/kg respectively. The single pollution index of heavy metals in soils follows the sequence of Cd > Zn > Pb > Cr > Cu, and geoaccumulation followed the sequence of Cd > Zn > Cu > Pb > Cr. The mathematic average value of comprehensive pollution index was 4.42. The order of single ecological risk index was Cd > Cu > Pb > Zn > Cr, and the comprehensive potential ecological index was 228.87, which illustrated that the potential ecological risk was in the moderate level and the Cd contribution rate to the potential risk was 94%, which was the major source of ecological risk in the research area.【Conclusion】 The soil is seriously contaminated with serious contamination of Cd, slight contamination of Zn and non-contamination of Pb, Cr and Cu. The source of heavy metals includes agricultural transport, traffic pollution and natural activities. [ABSTRACT FROM AUTHOR]

【Objective】 The present paper was conducted to verify the utilization value of wild tea germplasm resources and screen out excellent wild tea germplasm resources in Guizhou. 【Method】Major biochemical components of spring shoots and tea leaf traits of 73 wild tea germplasm resources from 32 distributing areas were analyzed. 【Result】10 tea lines of Q3, Q18, Q27, Q32, Q34, Q41, Q49, Q51, Q56 and Q66 were selected from 73 wild tea germplasm resources. Their free amino acid content of spring shoots and weight of 100 buds with one leaf both were higher than Fudingdabaicha(CK) significantly and their tea polyphenol and caffeine content was appropriate. Major biochemical components were basically the same between plant and cutting of 73 wild tea germplasm resources. The leaf palisade tissue to sponge tissue ratio and CTR of Q18, Q34, Q49 and Q56 tea lines were above 0. 6 and 0. 3 based on leaf anatomical structure respectively. 【Conclusion】 Q18, Q34, Q49 and Q56 tea lines with high free amino acid content in spring shoots, higher weight of 100 buds with one leaf and strong drought resistance should be utilized in tea production in Guizhou. [ABSTRACT FROM AUTHOR]

[Objective] The aim of this paper is to analyze the contents heavy metals and identify the risk assessment in soils around a large-scale swine farm. [Method]Taking soils around a large-scale swine farm with long-term application of swine manure as the research objects, heavy metal contents, including Cd, Cr, Pb, Hg, As, Cu and Zn were detected in 45 soil samples collected randomly. Then the soil environmental quality was analyzed and evaluated through Nemerow comprehensive pollution index and Hakanson potential ecological hazard index, so as to find the potential safety risks. [Result] The average contents of Cd,Cr, Pb, Hg, As,Cu and Zn were respectively 0.30,34.22,68.43,25.47,0.29,40.23 and 184.93 mg/kg in soils. Besides,the heavy metals Cd,As,Hg,Cu and Zn were higher than the standard of Grade II . Cd,Pb, As,Hg,Cu and Zn were higher than the standard of Guizhou background value. What is more,the single pollution index of the seven heavy metals in soil is (hi > Hg > Zn > Cu > As > Pb > Cr and Nemerow comprehensive pollution index of soil environment quality in the tested area was 1.01. The potential ecological risk index was As > Hg > Cd > Pb > Cu > Zn > Cr and potential ecological risk value was 109.88. [Conclusion] The assessment result of Nemerow comprehensive pollution index indicates that the soil was slightly polluted,which has been applied with the swine manure for the large-scale swine farm,such as polluted by Cd,Hg and Zn. The assessment result of Hakanson potential ecological hazard index still indicates that the soil was at a minor ecological risk level and the soil was in low potential ecological hazards. [ABSTRACT FROM AUTHOR]

【objective】This paper aims to provide references for eco-environment protection and sustainable development of Yunlaishan Karst natural area.【Method】 The phosphale-soluhilizing index，phosphatase activity，growlh status and carbon source ulilizalion were analyzed by isolating and purifying organophosphate bacteria from its soil.【Result 】The phosphorus uptake，phosphalase activity，gmwlh status and car-hon source efficiency of organic phosphorus bacteria (OPBS13, OPBS12, OFSB14, OPBS32) isolated from soil were different and the phospholytic activity, phosphatase activity and growth rate were as follows: 0PSB14 > OPBS32 > 0PBS12 > 0PBS13.【Conclusion】Phosphorus solubilizing ability and growth status of organophosphorus bacteria in Yunlai mountain, Shibin, (ruizhou province were related to ihe secretion of extracellular phosphatase by organophosphorus bacteria and were affected by the utilization of different carbon sources. [ABSTRACT FROM AUTHOR]

[Objective] This paper aimed to explore occurrence status and species of root knot nematodes in kiwifnjit orchards and lay a foundation for control of Meloidogyne incognita in Xiuwen county, Guizhou. [Method]The morphology, rDNA-ITS and specific sequence of root knot nematodes collected from main producing region of kiwifnjit in Xiuwen county were analyzed and identified. [Result] The perineal pattern of female root knot nematode adult had a high and square-shaped dorsal arch composed of wavy smooth lines from thin to thick and some lines were bifurcated on the side without an obvious lateral line. The similarity between sequence of specific amplified fragment and sequence of Meloidogyne incognita in NCBI Database (Accession number ;JN005841. 1. 1) reached 100 %,which indicated that the root knot nematode belonged to Meloidogyne incognita. [Conclusion] The root knot nematode endangering kiwifruit is Meloidogyne incognita mainly in Xiuwen county, Guizhou. [ABSTRACT FROM AUTHOR]

[Objective]This paper aims to rationally manage paddy soil nutrients, provide the scientific basis for rational fertilization in rice production and realize high production and high efficiency of rice production in Guizhou. [Method]The paddy soil nutrient management sub-area is divided by taking accumulated temperature, elevation, topography, soil type and soil nutrient as subarea indexes and using the second-grade subarea method based on GIS technology. [Result] The paddy soil is divided into six first-grade subareas according to the subarea indexes of topography, paddy soil type, effective accumulated temperature and elevation and nine soil nutrient management subareas are divided according to soil nutrient indexes based on the first-grade subarea results in Guizhou. [Conclusion] The complex effective accumulated temperature, topography, soil type and soil nutrient are orderly classified by planning management subarea of paddy soil nutrients in Guizhou. [ABSTRACT FROM AUTHOR]

【Objective】 The present paper aims to fully utilize local sorghum resources and provide the basic data for breeding of sorghum varieties with resistance to arthracnose in Guizhou. 【Method】 The resistance to arthracnose of 64 local sorghum varieties was identified by the natural inoculation method in the field. 【Result】B14 , B60 , C31, D1 and D8 with the second resistant grade is of the moderate resistance. 37 local varieties and Hongyingzi (CK) with the third resistant grade are of the moderate susceptible. 21 varieties with the fourth resistant grade are of the high susceptible. The yield of B1, D39 and D55 can reach 493. 6, 466. 9 and 555. 9 kg/667m2 , respectively. The yield of B14 was the same as Hongyingzi basically and the yield of other varieties was less than Hongyingzi. 【Conclusion】B14 , B60 , C31, D1 and D8 with the moderate resistance to arthracnose can be used in disease-resistant breeding and mechanism research of sorghum varieties. [ABSTRACT FROM AUTHOR]

【Objective】 The present paper aims to provide a reference for rational utilization and scientific fertilization of paddy soil in Guizhou. 【Method】 The temporal and spatial evolution characteristics of paddy soil nutrients was analyzed by ArcGIS geostatistics method and Kriging theoretical model based on the determination data of 177 944 representative topsoil samples from rice-growing areas of nine regions ,Guizhou. 【Result】 The organic matter, total nitrogen and rapidly available potassium content and pH of paddy soil present the declining trends and available phosphorus content shows a rising trend compared with the data from the second national soil survey (1991) from angle of time series. There are quite differences in available phosphorus and rapidly available potassium content and pH of paddy soil but less difference in organic matter and total nitrogen content from angle of spatial distribution. The acidic paddy soil of most regions in Southeastern Guizhou has a higher total nitrogen and organic matter content and lower rapidly available potassium content. The organic matter, total nitrogen and available phosphorus content of paddy soil in Sinan and Shiqian county is low. The water-logging paddy soil has higher nutrient content but the gleyed paddy soil has higher organic matter and total nitrogen content and the lowest available phosphorus and rapidly available potassium content. 【Conclusion 】The nutrient content and pH of paddy soil show a declining trend compared with in 1991. There is a certain difference in soil nutrients among different regions. The nutrients ' content of water-logging paddy soil is the highest among four different paddy soil types. [ABSTRACT FROM AUTHOR]