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To optimize the design of girder bridge, the proper cell number of the two-lane CSWs PC box girder was investigated. Based on the two-lane CSWs PC box girder bridge design parameter, the mechanical behaviors of single-room girder box with single cell to five cells were analyzed under four different load conditions by finite element analysis (FEA).The mid-span displacement in the bottom flange, the von-Mises shear stress in both corrugated web sides, the normal warping stress and the natural frequency of the box girders were obtained. The results show that multiplying cell numbers can increase the prestressing efficiency and decrease the von-Mises shear stress in both corrugated web sides in some cases. However, with the cell number increasing, the normal warping stress under eccentric load and the dead weight of the girder are increased dramatically. By simple formula method and principal component analysis method, the proper cell number for the two-lane CSWs PC box girder is suggested. [ABSTRACT FROM AUTHOR]